Micro- and Nanotechnology in Tissue Engineering

Coutinho, Daniela F.; Costa, Pedro F.; Neves, Nuno M.; Gomes, Manuela E.; Reis, Rui L.

doi:10.1007/978-3-642-02824-3_1

Cited by 13 publications

(13 citation statements)

References 154 publications

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“… Grooves/Channels Material Dimension Cell/tissue Remarks Ref. Width/Diameter Depth/Height PMMA 10, 25, 100 μm 330 nm HOBs Decreased nanogroove widths led to increased contact guidance, decreased adhesion and increased angiogenic gene expressions 78 , 79 2, 3, 6, 12 μm 0.2, 0.5, 1.1, 1.9 μm Baby hamster kidney cells, Increased alignment with increased depth and decreased with increased width. 21 , 80 PDMS 5, 10, 20, 60 μm 25 μm Human neural stem cell Increased alignment and induced neurite growth with decreased micropattern dimensions.…”

Section: Applications For Tissue Engineering and Cell Materials Interamentioning

confidence: 99%

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

Ermis

Antmen

Hasırcı

2018

Bioactive Materials

236

169

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Cell-substrate interactions play a crucial role in the design of better biomaterials and integration of implants with the tissues. Adhesion is the binding process of the cells to the substrate through interactions between the surface molecules of the cell membrane and the substrate. There are several factors that affect cell adhesion including substrate surface chemistry, topography, and stiffness. These factors physically and chemically guide and influence the adhesion strength, spreading, shape and fate of the cell. Recently, technological advances enabled us to precisely engineer the geometry and chemistry of substrate surfaces enabling the control of the interaction cells with the substrate. Some of the most commonly used surface engineering methods for eliciting the desired cellular responses on biomaterials are photolithography, electron beam lithography, microcontact printing, and microfluidics. These methods allow production of nano- and micron level substrate features that can control cell adhesion, migration, differentiation, shape of the cells and the nuclei as well as measurement of the forces involved in such activities. This review aims to summarize the current techniques and associate these techniques with cellular responses in order to emphasize the effect of chemistry, dimensions, density and design of surface patterns on cell-substrate interactions. We conclude with future projections in the field of cell-substrate interactions in the hope of providing an outlook for the future studies.

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Section: Applications For Tissue Engineering and Cell Materials Interamentioning

confidence: 99%

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

Ermis

Antmen

Hasırcı

2018

Bioactive Materials

236

169

View full text Add to dashboard Cite

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“…Moreover, to promote osteoinductivity in vitro, studies on structural characteristics of bones, such as the pore shape, size, distribution, surface topography, and roughness on the micro and nanoscale, have been conducted because of the importance of the structural understanding of bones [27,28,29,30]. However, research on the surface properties of implanted scaffolds that imitate the bone microstructures in vivo has rarely been conducted.…”

Section: Resultsmentioning

confidence: 99%

Assessment of the Inner Surface Microstructure of Decellularized Cortical Bone by a Scanning Electron Microscope

et al. 2019

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The microstructural changes of bones, which form a hierarchy of skeletal tissue, vary, depending on their condition, and are affected by the behaviors of bone cells. The purpose of this study is to assess the microstructural changes in the inner femoral surface of Sprague Dawley rats according to the conditions using a scanning electron microscope. Microstructural differences on the endocortical surface were observed in the characteristics of osteocytic canaliculi, bone fibers, and surface roughness, showing a rougher surface in old adults and an osteoporosis model by quantitative comparison. These results could be helpful for developing a basic understanding of the microstructural changes that occur on the bone surface under various conditions.

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“…Microfluidic bioreactors enable the relationship between process parameters such as channel size, flow rate, shear stress and cellular responses to be mapped simultaneously. 46,47 The key advantage from a bioprocess discovery perspective is that multiple different microsphere substrates and nutrient feeds can be screened in parallel in a closed and semi-automated system in microliter quantities, enabling even the study of tissue formation within a well-defined microenvironment using minimum quantities of reagents. In terms of application of microfluidic bioreactors for creation of engineered bone, Lee and colleagues created multi-channel microfluidic devices to enable real-time monitoring of mineralised 3D tissue-like structures by osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform

et al. 2020

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Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol % TiO2 and either 0 mol % CoO (CoO 0%) or 2 mol % CoO (CoO 2%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together with standard culture techniques, cell-material interactions were studied using a novel perfusion microfluidic bioreactor that enabled cell culture on microspheres, along with automated processing and screening of culture variables. While titanium doping was found to support hMSCs expansion and differentiation, as well as endothelial cell-derived vessel formation, additional doping with cobalt did not improve the functionality of the microspheres. Furthermore, the microfluidic bioreactor enabled screening of culture parameters for cell culture on microspheres that could be potentially translated to a scaled-up system for tissue-engineered bone manufacturing.

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Micro- and Nanotechnology in Tissue Engineering

Cited by 13 publications

References 154 publications

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

Micro and Nanofabrication methods to control cell-substrate interactions and cell behavior: A review from the tissue engineering perspective

Assessment of the Inner Surface Microstructure of Decellularized Cortical Bone by a Scanning Electron Microscope

Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform

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