Micro-column high-performance liquid chromatography and flame-based detection principles

McGuffin, V. L.; Novotný, Miloš V.

doi:10.1016/s0021-9673(00)82054-3

Cited by 46 publications

(17 citation statements)

References 16 publications

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“…Novotny and co-workers [191][192][193] introduced a flame-based thermionic detector (TID) as a detection technique in capillary LC, The microcolumn LC mobile phase is nebulized with a hydrogen/nitrogen mixture into a primary flame. The combustion products are combined with additional fuel gas and are transported into the secondary, analytical flame.…”

Section: Thermionic Detectionmentioning

confidence: 99%

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Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Thermionic Detectionmentioning

confidence: 99%

“…Detector designs for flame photometric detection are very similar to those of thermionic detectors [191,200]. The main disadvantage of flame photometric detectors is the sensitivity dependence of modifier concentrations.…”

Section: Flame Photometric Detectionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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“…However, this suffers from the concomitant reduction in sample capacity. 6.17.4 Microcapillary packed columns [116] These have mainly been developed by McGuffin and Novotny [120] and coworkers and are characterised by low column diameter to particle size ratios of 2 to 5. The practical limitation of capillary columns is caused by dispersion in the detector/ injector systems producing peak broadening.…”

Section: Open Tubular Columnsmentioning

confidence: 99%

High performance liquid chromatography

Braithwaite

Smith

1999

Chromatographic Methods

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“…However, information concerning the dependence of the column behavior on column dimensions is available only for packed SFC columns [15]. Acquisition of the corresponding information for capillary columns is difficult, because the necessary quantitative data are greatly influenced by extra-column effects such as sampling, and flow restriction at the end of the column [9].…”

Section: Dimensions Of Capillary Columnsmentioning

confidence: 99%

An attempt to extend ordinary capillary gas chromatography to supercritical fluid chromatography (SFC)

Grob

1983

J. High Resol. Chromatogr.

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SummarySince capillary columns with well immobilized stationary phases are expected to withstand contact with supercritical fluids, we wished to study their amenability to SFC. Simultaneously, we wished to learn how far SFC can be accomplished with the ordinary tools of capillary GC.The study demonstrates that truly supercritical, not just relatively high, pressure is required to ensure the typical effects ofSFC.Resultsobtainedwith sub-and supercritical pressureare compared and discussed. A comprehensive study of the parameters permitting SFC with capillary GC equipment showed a clear preference for COz as a carrier, FID detection, and oncolumn sampling. While no additional equipment is required, a critical feature is the flow restrictor to be mounted on the end of the column. The production and properties of this restrictor are discussedindetaiLlt isreasonabletohopethatSFCwith0.1 mm id capillary columns can be realized in the pressure range of 100-150 bar, where substances which cannot be eluted from a capillary colum under GC donditions are expected to be analyzed.

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Micro-column high-performance liquid chromatography and flame-based detection principles

Cited by 46 publications

References 16 publications

Microcolumn liquid chromatography: instrumentation, detection and applications

Microcolumn liquid chromatography: instrumentation, detection and applications

High performance liquid chromatography

An attempt to extend ordinary capillary gas chromatography to supercritical fluid chromatography (SFC)

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