Micro-Gripper: A new concept for a monolithic single-cell manipulation device

Garcés-Schröder, Mayra; Leester-Schädel, Monika; Schulz, Matthias; Böl, Markus

doi:10.1016/j.sna.2015.10.024

Cited by 28 publications

(9 citation statements)

References 40 publications

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“…The design of the microgripper is based on the microgripper toolbox developed at the Institute of Microtechnology, of the Technische Universität Braunschweig, Braunschweig, Germany, for technical and life science applications [ 3 , 4 , 7 ]. The microgripper consists entirely of SU-8, a UV-sensitive, high-contrast, epoxy-based negative-tone photoresist.…”

Section: Methodsmentioning

confidence: 99%

“…Remote-controlled microgrippers have appeared as a solution to this task, in particular when analytical methods, such as live-cell imaging or patch clamping, are intended, where the 3D cell aggregates have to be held in a constant position during the experiment. A limited variety of grippers for microscopic biological objects have been developed [ 3 , 4 , 5 ]. The intended use is often decisive for the choice of the actuation principle.…”

Section: Introductionmentioning

confidence: 99%

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An Immersible Microgripper for Pancreatic Islet and Organoid Research

et al. 2022

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To improve the predictive value of in vitro experimentation, the use of 3D cell culture models, or organoids, is becoming increasingly popular. However, the current equipment of life science laboratories has been developed to deal with cell monolayers or cell suspensions. To handle 3D cell aggregates and organoids in a well-controlled manner, without causing structural damage or disturbing the function of interest, new instrumentation is needed. In particular, the precise and stable positioning in a cell bath with flow rates sufficient to characterize the kinetic responses to physiological or pharmacological stimuli can be a demanding task. Here, we present data that demonstrate that microgrippers are well suited to this task. The current version is able to work in aqueous solutions and was shown to position isolated pancreatic islets and 3D aggregates of insulin-secreting MIN6-cells. A stable hold required a gripping force of less than 30 mN and did not affect the cellular integrity. It was maintained even with high flow rates of the bath perfusion, and it was precise enough to permit the simultaneous microfluorimetric measurements and membrane potential measurements of the single cells within the islet through the use of patch-clamp electrodes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Immersible Microgripper for Pancreatic Islet and Organoid Research

et al. 2022

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“…Devices have been made to tackle aspects of oocyte manipulation, including cumulus cell stripping and zona removal, and there have also been promising advances in robotic devices to automate ICSI (Zeringue et al, 2001(Zeringue et al, , 2005. One particularly creative device integrated both the holding pipette and injection needle used in ICSI with a micro-gripper tool (Garcés-Schröder et al, 2015). This platform is also equipped with a force sensor to measure oocyte mechanical properties during the injection process, thus integrating two separate procedures.…”

Section: Potential For High-throughput and Automated Measurement Devicesmentioning

confidence: 99%

Microfluidic analysis of oocyte and embryo biomechanical properties to improve outcomes in assisted reproductive technologies

Yanez

Camarillo

2016

MHR: Basic Science of Reproductive Medicine

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Measurement of oocyte and embryo biomechanical properties has recently emerged as an exciting new approach to obtain a quantitative, objective estimate of developmental potential. However, many traditional methods for probing cell mechanical properties are time consuming, labor intensive and require expensive equipment. Microfluidic technology is currently making its way into many aspects of assisted reproductive technologies (ART), and is particularly well suited to measure embryo biomechanics due to the potential for robust, automated single-cell analysis at a low cost. This review will highlight microfluidic approaches to measure oocyte and embryo mechanics along with their ability to predict developmental potential and find practical application in the clinic. Although these new devices must be extensively validated before they can be integrated into the existing clinical workflow, they could eventually be used to constantly monitor oocyte and embryo developmental progress and enable more optimal decision making in ART.

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“…Therefore, it has a broad application prospect in the fields of micro-assembly (Heriban et al, 2008), intelligent structure (Gao et al, 2020;Tanaka, 2014), precision machining (Lin et al, 2016), nanotechnology (Gu et al, 2016;Li et al, 2019), bioengineering (Zainal et al, 2015). For example, it is used in the assembly of micro-parts (Schro¨ter and Schmidt, 2018), the probe positioning of scanning probe microscope (Lu et al, 2018), the clamping of cells in bioengineering (Garce´s-Schro¨der et al, 2015;Kim et al, 2008). However, as a ferroelectric material, the piezoelectric actuator has nonlinear characteristics such as hysteresis and creep (Li et al, 2015;Sabarianand et al, 2020), and it is easy to oscillate under the action of step and pulse signals, which will affect the operation accuracy of micro-assembly and micro-operating system driven by a piezoelectric actuator.…”

Section: Introductionmentioning

confidence: 99%

Precision open-loop control of piezoelectric actuator

Nie

Cui

Huang

et al. 2021

Journal of Intelligent Material Systems and Structures

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Due to space constraints, some micro-assemblies and micro-operating systems cannot install sensors, so it is challenging to achieve closed-loop control. For this reason, a precision open-loop control strategy for piezoelectric actuators is proposed. Firstly, based on the PI model and the proposed threshold partition method, the hysteresis model of the piezoelectric actuator with rate-dependent and few operators is established. Then the hysteresis error of the piezoelectric actuator is compensated by the inverse model obtained. Secondly, the creep model of the logarithmic piezoelectric actuator with simple expression and few parameters is established. Then, a creep controller without demand inverse is designed to compensate for the creep error of the piezoelectric actuator. Finally, a ZVD (Zero Vibration Derivative) input shaping method with good robustness is given to eliminate the oscillation generated by the piezoelectric actuator under the action of the step signal. The experimental results show that the displacement error of piezoelectric actuator is reduced from −9.07 to 9.46 μm to −1.22 to 1.78 μm when the maximum displacement is 120 μm after hysteresis compensation; after creeping compensation, within the action time of the 1200 s, the displacement creep of the piezoelectric actuator was reduced from 5.5 μm before compensation to 0.3 μm; after the oscillation control, the displacement overshoot of the piezoelectric actuator is reduced to 0.6% of that before control.

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Micro-Gripper: A new concept for a monolithic single-cell manipulation device

Cited by 28 publications

References 40 publications

An Immersible Microgripper for Pancreatic Islet and Organoid Research

An Immersible Microgripper for Pancreatic Islet and Organoid Research

Microfluidic analysis of oocyte and embryo biomechanical properties to improve outcomes in assisted reproductive technologies

Precision open-loop control of piezoelectric actuator

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