Micro-Nano Bioactive Glass Particles Incorporated Porous Scaffold for Promoting Osteogenesis and Angiogenesis in vitro

Abstract

Constructing the interconnected porous biomaterials scaffolds with osteogenesis and angiogenesis capacity is extremely important for efficient bone tissue engineering. Herein, we fabricated a bioactive micro-nano composite scaffolds with excellent in vitro osteogenesis and angiogenesis capacity, based on poly (lactic-co-glycolic acid) (PLGA) incorporated with micro-nano bioactive glass (MNBG). The results showed that the addition of MNBG enlarged the pore size, increased the compressive modulus (4 times improvement), enhanced the physiological stability and apatite-forming ability of porous PLGA scaffolds. The in vitro studies indicated that the PLGA-MNBG porous scaffold could enhance the mouse bone mesenchymal stem cells (mBMSCs) attachment, proliferation, and promote the expression of osteogenesis marker (ALP). Additionally, PLGA-MNBG could also support the attachment and proliferation of human umbilical vein endothelial cells (HUVECs), and significantly enhanced the expression of angiogenesis marker (CD31) of HUVECs. The as-prepared bioactive PLGA-MNBG nanocomposites scaffolds with good osteogenesis and angiogenesis probably have a promising application for bone tissue regeneration.

Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

“…A gradient yield of Ca 2+ and Pi was also noted for other types of CaP-containing scaffolds [ 35 ], accompanied by the formation of an alkaline microenvironment that determines the realization of the osteogenic potential of MSCs [ 36 ]. This potential can be realized through various mechanisms, including CaR activity, the activation of osteoblastic differentiation and the formation of mineralization nodules in the ECM [ 6 ].…”

The Injectable Woven Bone-Like Hydrogel to Perform Alveolar Ridge Preservation With Adapted Remodeling Performance After Tooth Extraction

“…The ECM has a prominent interpore connectivity and an ideal bone substitute should provide a similar interconnected pore structure to support cell attachment and migration as well as the circulation of nutrients (Zadpoor, 2015;Zhang Y. et al, 2019;Zhu et al, 2019). The optimal pore size recommended is recommended over 300 µm and the porosity is preferably in the range from 70 to 90% (Turco et al, 2009;Tian et al, 2019). In our study, the alginate hydrogel was selected to achieve scaffold.…”

Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications

“…Nano-bioactive glass can also be added to the scaffolds for bone tissue engineering. Compared with microcrystalline bioactive glass, nano-bioactive glass can not only obtain a higher specific surface area and three-dimensional channel structure but can also increase the release of silicon ions and calcium ions to promote osteogenesis and angiogenesis [30,31].…”

“…In vitro study [17] Micro-nano bioactive glass particles In vitro study [31] Mesoporous silica nanoparticles In vitro study [32] Soft tissue wound healing Gold nanoparticles (1) In vitro study (2) In vivo wound model [14,38,43,44] Nano-sized bioactive glass (1) In vitro study (2) In vivo wound healing assay [39] Cerium oxide nanoparticle (1) In vitro study (2) In vivo wound model [42] the PCL nano-composite membranes incorporated with Zn-doped hollow mesoporous silica nanospheres…”