Micro QuEChERS-based method for the simultaneous biomonitoring in whole blood of 360 toxicologically relevant pollutants for wildlife

Abstract

• Validation of a method for the simultaneous quantification of 360 toxic chemicals in whole blood • One-step acetate buffered micro QuEChERS using acidified acetonitrile yielded recoveries N70%. • Only 250 μl of sample and quantification at the sub-ppb level makes it suitable for biomonitoring. • Verified in a series of 36 barn owls and 112 common kestrels, which test positive for 3-25 pollutants

Supporting dataset on the validation and verification of the analytical method for the biomonitoring of 360 toxicologically relevant pollutants in whole blood

“…The data reported in this table complement those included in Table 1 of the article by Rial-Berriel et al. [3] , where the parameters of identity and selectivity of each compound are shown. Now, in this table, the data of linearity (as a working range), the LOQ (set as the lowest point of the calibration curve that complied identity, bias and precision criteria), repeatability (as intraday RSD), and reproducibility (as interday RSD) are shown for each of the chemicals.…”

“…Then, the QuEChERS salts (150 mg anhydrous magnesium sulfate and 37.5 mg sodium acetate) were added and, the tubes were vortexed 30 s and vigorously manually shaken for 60 s. After a 5 min centrifugation, the supernatant was filtered (0.2 µm) and used directly for chromatographic analyses. The optimization of the procedure is detailed in the main article [3] .…”

“…The stationary phase in GC consisted of two fused silica ultra-inert capillary columns Agilent HP-5MS (15 m × 0.25 mm i.d., 0.25 µm film thickness), that were connected by a purged union, to allow the backflushing. All the conditions in which these apparatus were operated, as well as the optimization procedure, are described in detail in the main article [3] .…”

“…Therefore, we decided to use a 1: 1 mixture of both blood types to complete all validation experiments, following the recommendations of the SWGTOX guide. All the parameters of the validation process are shown in this article, except for the identity and selectivity parameters, which are shown in Table 1 of the main article [3] , and carryover, which is also explained in the main article. As the blank matrix was not completely free of all of the contaminants, the signal of the blanks was subtracted from fortified samples in all the experiments.…”

Dataset on the concentrations of anticoagulant rodenticides in raptors from the Canary Islands with geographic information

“…The method employed from January 2011 to November 2015 was a solid-liquid extraction followed of a LC-MS/MS analysis using a Thermo LC-MS/MS Accela Ultra instrument (Thermo Fisher Scientific Inc., USA) as previously described [5] . The method employed from December 2015 to May 2020 consisted on an extraction based on the QuEChERS method (Anastassiades et al., 2003), which has been fully validated in our laboratory followed by a LC-MS/MS analysis using an Agilent 1290 UHPLC (Agilent Technologies, Palo Alto, USA) coupled to an Agilent 6460 triple-quadrupole mass spectrometer, according to the previously described procedure [6] . All the quantitative data were obtained from at least two independent measurements.…”