Micro‐Raman spectroscopic study of organ cultured corneae

Siew, Diana C. W.; Clover, G. M.; Cooney, Ralph P.; Wiggins, Phillipa M.

doi:10.1002/jrs.1250260103

Cited by 15 publications

(10 citation statements)

References 25 publications

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“…The technique was utilized previously to study, e.g., protein and DNA conformations, drug interactions with bio-macromolecules and enzymes structure and action. 1,2 More recently it has been applied to determine the total water content in human organ-cultured corneas 3 and to characterize the ocular lens and its transformation from the normal to the cataractous state. 4 Using Raman spectroscopy, changes in the structural proteins and hydration state of the skin, hair and nail have recently been investigated by Gniadecka and co-workers.…”

Section: Introductionmentioning

confidence: 99%

Hydration of glutaraldehyde‐fixed pericardium tissue: Raman spectroscopic study

Jastrzębska

Wrzalik

Kocot

et al. 2003

J Raman Spectroscopy

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Raman spectroscopy was applied to investigate changes in the hydration state of chemically modified porcine pericardium tissue. Chemically modified tissues are used in construction of bioprostheses for replacement surgery including dermis, heart valves and blood vessels. The hydration state is a very important factor influencing specific mechanical and immunogenic properties of tissue derived materials. We analysed OH vibrational bands in the Raman spectra measured in the range 3000-3800 cm −1 for native and glutaraldehyde (GA) cross-linked porcine pericardium tissue. For deuterated samples Raman spectra were measured in the broadened wavenumber range 2100-3800 cm −1 . All spectra were deconvoluted using the minimum required three-Gaussian fit. Peak position, integrated intensity, separation between two main peaks d 1 -2 , integrated Gaussian OH component intensity ratio K = I 1 /I 2 and the integrated intensity fraction R = I/I total were determined to analyse changes in Raman OH and OD bands of the collagenous pericardium tissue upon GA treatment. It was found that the formation of collagen-GA cross-links causes an increase in the total water content of the tissue and an increase in the interaxial distance between collagen triple helices and introduces changes in the hydration shell surrounding the triple helix. Changes in the hydration shell are postulated to consist mainly of more sufficient formation of hexagonal than pentagonal water clusters in the space between two neighbouring triple helices. The OD and OH Raman bands measured for deuterated tissues revealed the changes in the distribution of water molecules in different subunits of collagen fibrils in the cross-linked pericardium tissue.

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Section: Introductionmentioning

confidence: 99%

Hydration of glutaraldehyde‐fixed pericardium tissue: Raman spectroscopic study

Jastrzębska

Wrzalik

Kocot

et al. 2003

J Raman Spectroscopy

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“…Bauer et al 29 found a decrease in corneal thickness of 50 µm in rabbit corneas exposed to an ambient environment after 30 min. Siew et al 37 detected an increase in hydration after excision and storage of human eye bank corneas in medium (corneal swelling). This work demonstrates the feasibility of real-time monitoring of the hydration changes in the living cornea, as illustrated by the OH/CH ratio versus time in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Examples are monitoring the healing process following PRK; monitoring corneal grafts; monitoring hydration status of the cornea while exploring new laser wavelengths for refractive keratectomy; determining changes of corneal hydration after the use of topically applied drugs; 39 following the reaction of corneal tissue on contact lens usage; and quality control and selection of eye bank eyes. 37 Maximum and minimum swelling properties of the corneal stroma could be studied and how stress events such as contact lenses or a cataract extraction would interfere with these properties. 40 -42 It might be possible that the forced dehydration with Muro 128  can be applied as a test for endothelial function.…”

Section: Discussionmentioning

confidence: 99%

Drug‐induced corneal hydration changes monitored in vivo by non‐invasive confocal Raman spectroscopy

Erckens

Jongsma

Wicksted

et al. 2001

J Raman Spectroscopy

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It is well established that the state of corneal hydration plays a crucial role in maintaining optimal vision. Therefore, any knowledge that can be obtained non-invasively about the status of corneal hydration could be of significant clinical value. A novel confocal Raman spectroscopic technique was used to monitor non-invasively drug-induced hydration changes in the rabbit cornea. The spectroscopic technique enables one to monitor the changes in water content of the cornea while the confocal probing reduces interference of signals from adjacent tissues and allows for measurement of corneal hydration at various depths. The corneal hydration is altered by applying a dehydrating agent (Muro 128  ) topically on the cornea. To determine the corneal hydration status, the OH/CH ratio between the Raman intensity of the water OH mode at 3390 cm −1 and the protein CH stretching mode at 2945 cm −1 is calculated. In the middle of the corneal stroma after 10 min, Muro 128  -treated corneas show an average decrease of about 30% in the OH/CH ratio (1.27 ± 0.13) compared with the untreated corneas (1.76 ± 0.09). In this in vivo model it is possible to monitor the hydration status of the living cornea using the Raman spectroscopic technique.

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“…A micro-Raman spectroscopic study of organcultured corneas was performed by Siew et al [58] to investigate if quality control of these eye bank corneas was feasible using this technique. It proved to be a sensitive technique for measurements in a non-contact fashion.…”

Section: Cornea and Scleramentioning

confidence: 99%

Raman Spectroscopy in Ophthalmology: From Experimental Tool to Applications In Vivo

et al. 2001

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Raman spectroscopy is a qualitative and quantitative optical technique for determining the molecular composition of matter. Improvements in spectroscopic instruments, especially the modality to detect low light level signals extended the Raman technique to biomedical applications, even in delicate structures like the eye. The purpose of this paper was to make an inventory of performed applications of Raman spectroscopy in biomedical science and especially in ophthalmology. A literature search was done using Medline, Current Contents, a patent server on the Internet, and references found in articles and patents. This search revealed a variety of Raman techniques and applications in biomedical research, and an increasing flow of articles starting in the late 1970s on Raman spectroscopy in ophthalmology. This increase in literature about Raman spectroscopy in ophthalmology feeds the expectation that this valuable technique will be introduced in the future into clinical practice.

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Micro‐Raman spectroscopic study of organ cultured corneae

Cited by 15 publications

References 25 publications

Hydration of glutaraldehyde‐fixed pericardium tissue: Raman spectroscopic study

Hydration of glutaraldehyde‐fixed pericardium tissue: Raman spectroscopic study

Drug‐induced corneal hydration changes monitored in vivo by non‐invasive confocal Raman spectroscopy

Raman Spectroscopy in Ophthalmology: From Experimental Tool to Applications In Vivo

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