Raman Spectroscopy in Ophthalmology: From Experimental Tool to Applications In Vivo

Erckens, Roel J.; Jongsma, Franciscus H. M.; Wicksted, James P.; Hendrikse, Fred; March, Wayne F.; Motamedi, Massoud

doi:10.1007/pl00011360

Cited by 48 publications

(36 citation statements)

References 154 publications

(164 reference statements)

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“…The occurrence of small spectral features in the anterior chamber of the eye which are native to this medium were to be expected since different biological molecules with various Raman activity already exist in this medium [11,13]. In order to be able to distinguish the specific peaks of the antibiotic ceftazidime and antifungal amphotericin B, we had to search for those peaks specific to these drugs that would not coincide with the inherent peaks of the aqueous humor.…”

Section: Resultsmentioning

confidence: 99%

“…Raman spectroscopy has been successfully utilized in rabbit eyes for different types of investigations [11,20] and it may offer an effective tool to non-invasively assess the concentration of various intravitreal drugs as tested in both the aqueous humor and vitreous humor. Bauer et al have already shown the feasibility of Raman spectroscopy in detecting the drug Trusopt in the tear film of the eye in a pharmacokinetic study [21] and we have demonstrated its potential in monitoring the antiviral drug Ganciclovir in a previous study [13].…”

Section: Discussionmentioning

confidence: 99%

“…In the biomedical field, the presence of molecules that exhibit unique Raman signatures can be detected using Raman spectroscopy [7][8][9][10][11]. Raman spectroscopy offers the potential to detect a specific molecule based upon the characterization of the vibrational frequencies of various molecules [12].…”

Section: Introductionmentioning

confidence: 99%

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Non‐invasive monitoring of commonly used intraocular drugs against endophthalmitis by raman spectroscopy

et al. 2003

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Non‐invasive monitoring of commonly used intraocular drugs against endophthalmitis by raman spectroscopy

et al. 2003

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“…Earlier reports utilizing Raman spectroscopy for ophthalmological investigation focused on detection of human macular pigment and glutamate in the eyes, in which the excitation laser wavelength was selected to resonate with the vibrational modes of the pigment/glutamate molecules, and an enhanced Raman signal was achieved. 25,27 To acquire a Raman signal directly from the RGC cells without the resonance enhancement effects, the autofluorescence background has to be removed before the analysis of the vibrational bands takes place. The most common methods for background removal, as the one currently used in this study, are based on digital signal subtraction utilizing the common feature of fluorescent backgrounds-a smooth function of the emission wavelength.…”

Section: Discussionmentioning

confidence: 99%

Exploring Raman spectroscopy for the evaluation of glaucomatous retinal changes

Wang

Grozdanić²,

Harper³

et al. 2011

J. Biomed. Opt.

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Abstract. Glaucoma is a chronic neurodegenerative disease characterized by apoptosis of retinal ganglion cells and subsequent loss of visual function. Early detection of glaucoma is critical for the prevention of permanent structural damage and irreversible vision loss. Raman spectroscopy is a technique that provides rapid biochemical characterization of tissues in a nondestructive and noninvasive fashion. In this study, we explored the potential of using Raman spectroscopy for detection of glaucomatous changes in vitro. Raman spectroscopic imaging was conducted on retinal tissues of dogs with hereditary glaucoma and healthy control dogs. The Raman spectra were subjected to multivariate discriminant analysis with a support vector machine algorithm, and a classification model was developed to differentiate disease tissues versus healthy tissues. Spectroscopic analysis of 105 retinal ganglion cells (RGCs) from glaucomatous dogs and 267 RGCs from healthy dogs revealed spectroscopic markers that differentiated glaucomatous specimens from healthy controls. Furthermore, the multivariate discriminant model differentiated healthy samples and glaucomatous samples with good accuracy [healthy 89.5% and glaucomatous 97.6% for the same breed (Basset Hounds); and healthy 85.0% and glaucomatous 85.5% for different breeds (Beagles versus Basset Hounds)]. Raman spectroscopic screening can be used for in vitro detection of glaucomatous changes in retinal tissue with a high specificity. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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“…Vibrational spectroscopies, Raman and infrared, have been extensively used in biology and medicine as analytic and diagnostic tools from their early beginnings [5], [6], [7] and [8]. Rapid computerisation and the discovery of laser accelerated the number of these studies reported in books and conference proceedings [9], [10] and [11].…”

Section: Introductionmentioning

confidence: 99%

Development of cataract caused by diabetes mellitus: Raman study

Furić

Mohaček-Grošev

Hadžija

2005

Journal of Molecular Structure

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Diabetes mellitus succeeded by diabetic cataract was induced to experimental animals (Wistar rats) by applying an Alloxan injection. Eye properties deterioration were monitored from clinical standpoint and using Raman and infrared spectroscopies. All cases of developed cataract were followed by important changes in vibrational spectra, but Raman spectroscopy proved to be more useful because of larger number of resolved bands. Each kth Raman spectrum of diseased lens (in our notation k denotes disease age and cataract degree as described in chapter Alloxan diabetes) can be expressed as a sum of the Raman spectrum of healthy lens, I R , multiplied by a suitable constant c k , and the fluorescent background spectrum, I FB . We introduce the ratio of integrated intensities I FB and c k *I R as a physical parameter called fluorescent background index F FB . It turns out that F FB grows as cataract progresses and has its maximum at approx. 4, whence it decreases. F FB values are larger for 200-1800 cm −1 spectral interval than for 2500-4000 cminterval.In the same manner another quantity called water band index F W is defined for each Raman spectrum of diseased lens in the 2800-3730 cm −1 interval. It is the ratio of the integrated intensity from 3100 to 3730 cm −1 (water band interval) divided by the integrated intensity of the 2800-3100 cm −1 interval (C-H stretching region). F W increases monotonously with cataract progression with maximum at the end of monitored period (5 months).These two indices helped us to formulate a model describing disease development from the earliest molecular changes to its macroscopic manifestation. As glucose and other small saccharide molecules enter the lens tissue, they bind to crystallin and other proteins via O -and Sglycosidic linkages which occur probably at tyrosine and cystein sites. In Raman spectrum this corresponds to broad bands at 540 and 1100 cm −1 which grow together with the fluorescent background, because both contributions originate in nonenzimatically glycated proteins. The maximum of possible binding ends after approximately 4 months (cataract degree 4), but the water continues to enter the tissue and resides in water agglomerates.The lens impairing caused by fluorescent light scattering on aberrant glycoproteins and other fluorescent centers appears first and is usually associated with the ageing cataract, while deterioration of lens properties caused by increased binding of water steadily rises with glucose Furić, K., Mohaček-Grošev, V., Hadžija, M. (2005), ''Development of cataract caused by diabetes mellitus: Raman study '' Journal of Molecular Structure, and is characteristic of diabetic cataract. This interpretation is in agreement with electron microscopy results of other groups and with our preliminary findings obtained with light microscopy.

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Raman Spectroscopy in Ophthalmology: From Experimental Tool to Applications In Vivo

Cited by 48 publications

References 154 publications

Non‐invasive monitoring of commonly used intraocular drugs against endophthalmitis by raman spectroscopy

Non‐invasive monitoring of commonly used intraocular drugs against endophthalmitis by raman spectroscopy

Exploring Raman spectroscopy for the evaluation of glaucomatous retinal changes

Development of cataract caused by diabetes mellitus: Raman study

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