micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation

Tijunelyte, Inga; Malbec, Rémi; Chami, Bayan; Cacheux, Jean; Dez, Christophe; Leïchlé, Thierry; Cordelier, Pierre; Bancaud, Aurélien

doi:10.1016/j.bios.2021.112992

Cited by 7 publications

(12 citation statements)

References 32 publications

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“…Malignant insidious proliferation and metastasis were the primary reasons for unsatis ed prognosis in pancreatic cancer patients. Lots of studies have indicated that miRNAs played essential roles in metastasis and malignant proliferation of pancreatic cancer [22,23]. This study showed miR-130b was obviously decreased in the pancreatic cancer samples.…”

Section: Disscusionmentioning

confidence: 72%

Construction and Investigation of an lncRNA-miRNA-mRNA Regulatory Network of Pancreatic Cancer via bioinformatics analysis

yang

Tang

et al. 2022

Preprint

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PurposePancreatic cancer causes a malignant digestive tumour with an unfavourable prognosis and insidious onset. The regulation mechanisms of pancreatic cancer were connected to the abnormal accommodate of diverse signaling pathways. Studies had increasingly indicated that long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNA (mRNAs) could play crucial roles during tumorigenesis. This study designs to detect functional genes and determine the potential molecular mechanisms of pancreatic cancer through bioinformatics.MethodsWe made a comprehensive comparison on the RNA-sequencing using the Gene Expression Omnibus (GEO) and established a dysregulated lncRNA-miRNA-mRNA network. In addition, several functional analyses in the competing endogenous RNAs (ceRNA) network were conducted by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, survival analysis and clinical feature analysis were made by Gene Expression Profiling Interactive Analysis (GEPIA) and linkedOmics. Proliferation, migration tests were selected to examine the roles of mir-130b in pancreatic cancer. Results23 lncRNAs, 40 miRNAs and 1613 mRNAs were aberrantly expressed in pancreatic cancer. We constructed a dysregulated ceRNA network with 11 lncRNAs, 19 miRNAs, and 66 mRNAs. A group of 121 interconnections was constructed in the ceRNA network. These upregulated DEmRNAs were mainly enriched in the mitotic spindle, were involved the transcription by RNA polymerase II, were related to E-box binding and participated in bacterial invasion of epithelial cells and yersinia infection etc. in contrast, the these upregulated DEmiRNAs were mainly enriched in spanning component of the plasma membrane, were involved in pyrimidine nucleobase catabolic process, were related to pyridoxal phosphate binding and participated in beta-Alanine metabolism and propanoate metabolism etc.. The survival analysis showed that 1 lncRNAs, 4 miRNAs, and 18 mRNAs might be potential factors for the prognostic prediction of pancreatic cancer. The strong positive relationships were found between candidate lncRNAs and candidate mRNAs targeted by miR-130b, CYTOR interacts with FRMD6 and TCF4, while HOXA11-AS interacts with MET regulated by miR-130b. MiR-130b was found that its overexpression could inhibit the proliferation and migration of pancreatic cancer cells, and lower expression of miR-130b was connected with longer survival of pancreatic cancer patients.ConclusionOur research provided further acknowledges the molecular mechanisms underlying pancreatic cancer and laid strong ground for improving pancreatic cancer diagnosis and prognosis by establishing the lncRNA-miRNA-mRNA regulatory network via bioinformatics analysis.

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Section: Disscusionmentioning

confidence: 72%

Construction and Investigation of an lncRNA-miRNA-mRNA Regulatory Network of Pancreatic Cancer via bioinformatics analysis

yang

Tang

et al. 2022

Preprint

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“…In order to extract the LoD, we used the method described in ref. 27 . Briefly, we recorded 10 images before the application of the electric field, and used the corresponding intensity signal to define the background and its standard deviation.…”

Section: Microscopy and Image Analysismentioning

confidence: 99%

“…The use of electric fields neither induces detectable temperature change in the silicon chip nor does it alter DNA hybridization reactions, as we showed in ref. 27 .…”

Section: Operation Of the Concentration Modulementioning

confidence: 99%

“…They can thus be flushed out, and selective target detection is achieved, as was demonstrated in ref. 27 . Notably, the resulting theoretical diagram is in qualitative agreement with that reported in ref.…”

Section: Operation Of the Concentration Modulementioning

confidence: 99%

“…In line with this biosensing technology based on a concentration module, we recently developed the µLAS technology (µ-Laboratory for DNA analysis and separation), which enables DNA size separation, concentration, and purification in a microchannel using bi-directional electrohydrodynamic actuation [23][24][25][26] . This technology has been exploited to detect nucleic acids by the selective concentration of the complex formed between the target molecule and a complementary MB 27 . We reported an LoD of 2 pM within 30 seconds.…”

Section: Introductionmentioning

confidence: 99%

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Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography

et al. 2022

Self Cite

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Speeding up and enhancing the performances of nucleic acid biosensing technologies have remained drivers for innovation. Here, we optimize a fluorimetry-based technology for DNA detection based on the concentration of linear targets paired with probes. The concentration module consists of a microfluidic channel with the shape of a funnel in which we monitor a viscoelastic flow and a counter-electrophoretic force. We report that the technology performs better with a target longer than 100 nucleotides (nt) and a probe shorter than 30 nt. We also prove that the control of the funnel geometry in 2.5D using grayscale lithography enhances sensitivity by 100-fold in comparison to chips obtained by conventional photolithography. With these optimized settings, we demonstrate a limit of detection of 4 fM in 30 s and a detection range of more than five decades. This technology hence provides an excellent balance between sensitivity and time to result.

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Mn-MOF catalyzed multi-site atom transfer radical polymerization electrochemical sensing of miRNA-21

Wang

Liu

et al. 2023

Microchim Acta

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micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation

Cited by 7 publications

References 32 publications

Construction and Investigation of an lncRNA-miRNA-mRNA Regulatory Network of Pancreatic Cancer via bioinformatics analysis

Construction and Investigation of an lncRNA-miRNA-mRNA Regulatory Network of Pancreatic Cancer via bioinformatics analysis

Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography

Mn-MOF catalyzed multi-site atom transfer radical polymerization electrochemical sensing of miRNA-21

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