Micro-Rna as Markers of Pre-Cancer Diseases Progression in Cervical Cancer

Димитриади, Т. А.; Бурцев, Д. В.; Dzhenkova, Elena A.; Кутилин, Денис С.

doi:10.17513/spno.29529

Cited by 4 publications

(6 citation statements)

References 11 publications

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“…МикроРНК hsa-miR-196b-5p и hsa-miR-34a-5p таргетируют гены CDKN2A, MKI67 и TOP2A, CD82 соответственно. При этом общепринято [5], что повышение уровня мкРНК должно приводить к снижению уровня экспрессии гена-мишени и наоборот. Ми-кроРНК hsa-miR-196b-5p снижает свою экспрессию у пациенток с CIN3 и CIS, при этом экспрессия CDKN2A и MKI67 повышается только в подгруппе CIN3, а в CIS не изменяется либо снижается соответственно.…”

Section: Discussionunclassified

“…Прогрессирование до интраэпителиальных предраковых заболеваний происходит из-за множественных нарушений в сигнальных путях, регулирующих клеточный цикл, вызванных избыточной экспрессией вирусных белков E6 и E7, которые функционально инактивируют продукты генов RB и TP53. Данный процесс является многоэтапным и включает ряд генетических и эпигенетических изменений, в том числе изменения транскрипционной активности генов и микроРНК (мкРНК) [5].…”

Section: Introductionunclassified

“…МикроРНК -короткие некодирующие РНК, явля ющиеся эпигенетическими регуляторами, спе цифичны ми для определенных тканей, которые модулируют экспрессию генов путем взаимодействия с комплементарными нуклеотидными последовательностями мишеней матричной РНК (мРНК), что либо катализирует разрушение мРНК, либо ингибирует трансляцию мРНК в белок [5].…”

Section: Introductionunclassified

“…В настоящее время недостаточно известно о специфических мкРНК, задействованных в развитии CIN (в переходе от CIN1 к CIN2 и CIN3), и механизмах их регуляции [6]. Вероятно, аберрантная экспрессия мкРНК при развитии CIN является результатом инфицирования ВПЧ [5].…”

Section: Introductionunclassified

“…Проведенный нами ранее метаанализ позволил выделить 8 мкРНК (hsa-miR-1246, hsa-miR-145-5p, hsa-miR-196b-5p, hsa-miR-34a-5p, hsa-miR-20a-5p, hsa-miR-21-5p, hsa-miR-375-5p, hsa-miR-96-5p), обладающих потенциальной значимостью в прогрессировании предраковых заболеваний в РШМ [5].…”

Section: Introductionunclassified

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Differential expression of microRNAs and their target genes in cervical intraepithelial neoplasias of varying severity

Димитриади

Бурцев

Dzhenkova³

et al. 2020

Usp. mol. onkol

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Background. Currently, little is known about the specific microRNAs involved in the development of cervical intraepithelial neoplasia (CIN1, 2, 3) and the transition to cancer in situ (CIS). Our meta-analysis allowed us to isolate 8 microRNAs (hsa-miR-1246, hsa-miR- 145-5p, hsa-miR-196b-5p, hsa-miR-34a-5p, hsa-miR-20a-5p, hsa-miR-21-5p, hsa-miR-375-5p, hsa-miR-96-5p) with potential significance in the progression of precancerous diseases to cervical cancer. Objective: to analyze the expression features of hsa-miR-1246, hsa-miR-145-5p, hsa-miR-196b-5p, hsa-miR-34a-5p, hsa-miR-20a-5p, hsa-miR-21-5p, hsa-miR-375-5p, hsa-miR-96-5p and their target genes, as well as genes associated with them in common signaling pathways in the tissues of the cervix in patients with CIN1–3 and CIS. Materials and methods. To assess the expression level of microRNA and matrixRNA, the quantitative polymerase chain reaction in real time method was used. Data analysis was carried out in the Python programming language using the SciPy library. Search for target genes was performed using the TarPmiR algorithm and the overrepresentation of microRNAs in signaling pathways (Over-Representation Analysis) was analyzed. To identify genes associated with target genes in common signaling pathways, GIANT (Genome-scale Integrated Analysis of gene Networks in Tissues) and network integration with several associations algorithms were used. Results. For microRNAs miR-145, miR-196b, miR-34a, miR-20a, miR-21, miR-375 and miR-96 a decrease in expression was found in the subgroup of patients with CIS, while for 4 microRNAs (miR-145, miR-34a, miR-20a and miR-375), an increase in the expression level was found for CIN1, 2. The detected features of microRNA expression in subgroups of patients with CIN1–3 and CIS also affected the expression of their target genes (CDKN2A, MKI67, TOP2A and CD82), as well as the genes associated with them in common signaling pathways (PGK1, THBS4 (TSP4) and ECM1). Conclusion. Thus, the study revealed that each degree of CIN is characterized by its own specific molecular profile – the differential expression of microRNAs, their target genes and the genes associated with them in the general signaling pathways.

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Section: Discussionunclassified

Section: Introductionunclassified

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Differential expression of microRNAs and their target genes in cervical intraepithelial neoplasias of varying severity

Димитриади

Бурцев

Dzhenkova³

et al. 2020

Usp. mol. onkol

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Genetic and epigenetic characteristics of non-muscle invasive and muscle invasive bladder cancer in patients infected by human papillomavirus: literature review

et al. 2023

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Infectious diseases and chronic inflammation are important risk factors for the development of malignant tumors in humans. One of the key infectious agents involved in human oncogenesis is the human papillomavirus (HPV). Non-muscle invasive bladder cancer is defined as a superficial neoplasia limited to the mucosa, aggravated by recurrence in 80 % of cases and progression in 30 % of cases. The development of this disease is associated with the influence of various carcinogenic agents, including HPV. Currently, a direct relationship has been revealed between the presence of viral DNA in the tumor tissue of the bladder and markers of proliferative activity, angiogenesis, and apoptosis factors. More and more researchers believe in the involvement of the virus in the development of recurrent forms of bladder cancer and the emergence of its invasive/poorly differentiated forms. Improving the diagnosis and postoperative monitoring of non-muscle invasive and muscle invasive bladder cancer is not possible without the improvement of minimally invasive molecular methods, which requires an understanding of the molecular mechanisms of HPV-associated carcinogenesis.Therefore, this review focuses on the analysis of the molecular mechanisms of HPV effect on progression of non-muscle invasive and muscle invasive bladder cancer. The features of miRNA expression in patients with papillomavirus infection of high oncogenic risk types and non-muscle invasive or muscle invasive bladder cancer are considered in detail. In particular, the role of miR-34а, -218, -20a, -424, -200a, -205-5p, -944, -100, -99a, -202, -30a, -145-5p, -195 and -199a-5 is described in the development and progression of bladder cancer. The mechanisms of disruption in the functioning of key cell signaling pathways during HPV integration in patients with bladder cancer, including changes in gene copy number and methylation level, are also considered.However, the number of HPV-positive tumor specimens that have been comprehensively analyzed using genome-wide studies in the literature remains small. Larger patient cohorts would be useful to further refine HPV-associated integration events and genomic changes, as well as to study clinical manifestations of the consequences of these alterations. Further research on the clinical implications of the observed genomic changes is needed to accurately stratify patients for targeted therapy, radiation and chemotherapy.

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Molecular Genetic Basis of Cervical Cancer

Lenkova,

Khusainova,

Minniakhmetov

2023

Mol Med Rus

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Introduction. Cervical cancer is one of the most common cancers of the female reproductive system, killing more than 250,000 women each year. The etiological agent that causes cervical cancer is well known. HPV infection with high oncogenic risk is the cause of more than 99% of cases of the disease. However, not all infections lead to cervical cancer: in the vast majority of women, the virus eliminates itself spontaneously within 2 years. Formation of HPV-associated cervical cancer depends on genetic and epigenetic factors, but the molecular pathogenesis of the disease is still insufficiently disclosed. The aim of the article is to analyze, systematize and summarize the available literature on the problems of prevalence, diagnosis, treatment, prevention, molecular genetic component. Material and methods. When writing this work, the main Internet resources were used: scientific electronic library (e-Library), Scopus, PubMed. The literature search was performed mainly for the previous 10 years. Results: The review contains articles describing genetic and epigenetic mechanisms of cervical cancer, modern methods of molecular-genetic diagnostics, as well as available statistical data on prevalence, prospects of prevention, diagnosis and treatment of this malignant disease.

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Micro-Rna as Markers of Pre-Cancer Diseases Progression in Cervical Cancer

Cited by 4 publications

References 11 publications

Differential expression of microRNAs and their target genes in cervical intraepithelial neoplasias of varying severity

Differential expression of microRNAs and their target genes in cervical intraepithelial neoplasias of varying severity

Genetic and epigenetic characteristics of non-muscle invasive and muscle invasive bladder cancer in patients infected by human papillomavirus: literature review

Molecular Genetic Basis of Cervical Cancer

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