Micro-scale extraction procedure for standardized screening of fungal metabolite production in cultures

Smedsgaard, Jørn

doi:10.1016/s0021-9673(96)00803-5

Cited by 398 publications

(282 citation statements)

References 11 publications

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“…Isolates were grown on CYA and YES for 7-14 days and three or more plugs were cut out of a colony for extraction. Extractions were performed as described by Smedsgaard (1997). Extracts were analysed by HPLC with diode array UV-VIS detection using alkylphenone retention indices (Frisvad & Thrane, 1987).…”

Section: Methodsmentioning

confidence: 99%

Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter

Houbraken

López-Quintero

Frisvad

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Several species of the genus Penicillium were isolated during a survey of the mycobiota of leaf litter and soil in Colombian Amazon forest. Five species, Penicillium penarojense sp. nov. (type strain CBS 113178T = IBT 23262T), Penicillium wotroi sp. nov. (type strain CBS 118171T = IBT 23253T), Penicillium araracuarense sp. nov. (type strain CBS 113149T = IBT 23247T), Penicillium elleniae sp. nov. (type strain CBS 118135T = IBT 23229T) and Penicillium vanderhammenii sp. nov. (type strain CBS 126216T = IBT 23203T) are described here as novel species. Their taxonomic novelty was determined using a polyphasic approach, combining phenotypic, molecular (ITS and partial β-tubulin sequences) and extrolite data. Phylogenetic analyses showed that each novel species formed a unique clade for both loci analysed and that they were most closely related to Penicillium simplicissimum, Penicillium janthinellum, Penicillium daleae and Penicillium brasilianum. An overview of the phylogeny of this taxonomically difficult group is presented, and 33 species are accepted. Each of the five novel species had a unique extrolite profile of known and uncharacterized metabolites and various compounds, such as penicillic acid, andrastin A, pulvilloric acid, paxillin, paspaline and janthitrem, were commonly produced by these phylogenetically related species. The novel species had a high growth rate on agar media, but could be distinguished from each other by several macro- and microscopical characteristics.

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Section: Methodsmentioning

confidence: 99%

Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter

Houbraken

López-Quintero

Frisvad

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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“…Fungal cultures were extracted by micro-scale extraction (Smedsgaard 1997) method based on agar plugs (6 mm). Two agar plugs were cut out of each colony (CYA, YES) from the center and from the edge of the colony.…”

Section: Analysis Of Secondary Metabolitesmentioning

confidence: 99%

Alternaria mycotoxins associated with grape berries in vitro and in situ

2013

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The aim of this study was to assess the production of secondary metabolites of Alternaria species isolated from grape berries and determined the occurrence of Alternaria mycotoxins in vitro and in dried berries. Direct morphological examination on different media was used for identification and HPLC/MS method for quantification of Alternaria mycotoxins. We isolated A. alternata and A. tenuissima and randomly selected strains in vitro and in dried berries. In vitro we identified the production of Alternaria metabolites, included mycotoxins: alternariol, alternariol methylether, tenuazonic acid. Beside that one in dry berries were in measurable concentrations: macrosporin A, tentoxin and altenuete (I, II).

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“…All cultures were incubated for 7 days in the dark at 25°C. For metabolite analysis three agar plugs were extracted as described previously (Frisvad 1987) and analysed by high-performance liquid chromatography with diode array detection (Frisvad and Thrane 1987) as modified by Smedsgaard (Smedsgaard 1997). The metabolites found were compared to a spectral UV library made from authentic standards examined under the same conditions, and the retention indices were compared with those of the standards.…”

Section: Metabolite Analysismentioning

confidence: 99%

Aspergillus vadensis, a new species of the group of black Aspergilli

Vries

Frisvad

Vondervoort

et al. 2005

Antonie Van Leeuwenhoek

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A strain from the group of black Aspergilli was analysed in detail to determine the species to which it belongs. A detailed analysis of morphology, RFLP patterns and metabolite profiles was carried out. In addition, a phylogenetic tree was constructed for the black Aspergilli using the ITS and the b-tubulin sequences of the individual strains. The new species differs by its poor growth on glycerol and galacturonate and its unique extrolite profile consisting of aurasperone B, nigragillin, asperazine and kotanins. RFLP analysis using three genes as probes also resulted in a unique pattern. These data indicate that the strain was closely related but not identical to Aspergillus foetidus, Aspergillus niger and Aspergillus tubingensis. It was therefore designated as a novel species and named Aspergillus vadensis.

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Micro-scale extraction procedure for standardized screening of fungal metabolite production in cultures

Cited by 398 publications

References 11 publications

Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter

Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter

Alternaria mycotoxins associated with grape berries in vitro and in situ

Aspergillus vadensis, a new species of the group of black Aspergilli

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