Microalgae cultivation using palm oil mill effluent as growth medium for lipid production with the effect of CO2 supply and light intensity

Bhuyar, Prakash; Sundararaju, Sathyavathi; Hasbi, Ab. Rahim Mohd; Ramaraj, Rameshprabu; Maniam, Gaanty Pragas; Nagarajan, G.

doi:10.1007/s13399-019-00548-5

Cited by 80 publications

(40 citation statements)

References 42 publications

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“…Cyanobacteria was cultivated in 2-L Erlenmeyer's flask contained BG-11 medium and incubated in the racks sourced with fluorescent lamps as a light source. The photo-incubation was carried out at 30 °C and with illuminated at light intensities 12 h dark period measured by LI-250 Light meter [17].…”

Section: Microalgae Mass Cultivationmentioning

confidence: 99%

“…Oven temperature was held isothermal at 80 °C for 1 min, then increased to 300 °C at a rate of 4 °C /min and held isothermal for 5 min. MS, (Agilent Technologies 5975C model) conditions were followed: ion source temperature of 200 °C; interface temperature of 300 °C; mass range of 40-1000 mass units [17,23]. The individual peaks formed were identified by comparing their retention time, as well as their mass spectra with the Fienn.…”

Section: Gas-chromatography With Mass Spectrometer Analysismentioning

confidence: 99%

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Exploration of bioactive compounds and antibacterial activity of marine blue-green microalgae (Oscillatoria sp.) isolated from coastal region of west Malaysia

et al. 2020

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Cyanobacteria is blue-green microalgae that produces a variety of secondary metabolites such as antibiotic, cytotoxic and antimicrobial agents. Present investigation proposed to study an extraction of active agents from Cyanobacteria and its antibacterial activity. The Cyanobacteria were collected, and isolated colony was found to be Oscillatoria sp. and it was grown in BG 11 medium for mass cultivation. Then the centrifuged biomass was weighed and used for extraction of bioactive compounds. GCMS analysis from Oscillatoria sp. determines fatty acid, triazine derivatives, pyridine derivatives, acridine derivatives. The result revealed that Oscillatoria sp. can play crucial roles as antibacterial agents because the methanolic extract are inhibiting the growth of S. aureus for gram-positive whereas P. aeruginosa for gram-negative bacteria. The crude extract was effective against S. aureus with the highest concentration, 100 mg/ml where area of the zone was 14.10 ± 0.8 mm. The crude extract actively inhibited P. aeruginosa at 75 and 100 mg/ml with highest area of inhibition were in the average of 13.23 ± 0.1 and 15.43 ± 0.2 mm. Finally, the antimicrobial activity was carried out by disc diffusion method with different concentrations of active compounds shows the MIC value in gram-positive bacteria was 30 µg/ml whereas for gram-negative bacteria was 25 µg/ml. Cyanobacteria extract should be preferred and considered to be new natural drug or antibiotics in pharmaceutical and health care industry. This coastal region should be studied further by researchers to study more information and benefits in the future.

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Section: Microalgae Mass Cultivationmentioning

confidence: 99%

Section: Gas-chromatography With Mass Spectrometer Analysismentioning

confidence: 99%

Exploration of bioactive compounds and antibacterial activity of marine blue-green microalgae (Oscillatoria sp.) isolated from coastal region of west Malaysia

et al. 2020

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“…The inoculums were prepared by taking at least three to five well-isolated colonies of the same morphology with a sterile loop and transferred into a tube containing 4 to 5 ml of Potato dextrose broth. The turbidity of the suspension is adjusted until it achieved the turbidity of the 0.1 McFarland standard (Bhuyar et al, 2019b). This resulted in a suspension containing approximately of 1 x 108 CFU/ml.…”

Section: Fungal Inoculum Preparationmentioning

confidence: 99%

“…To test the bioactive compounds, present, preliminary phytochemical analysis was carried out by using the standard method to identify the components in the various plants extracts of Centella asiatica. The methanol extracts of C. asiatica was subjected to the analysis of chemical components by gas chromatographymass spectrometer (GC-MS) (Ramli et al, 2020;Bhuyar et al, 2019b). A hightemperature column (Inert cap 1MS; 30 m x 0.25 mm id x 0.25μm film thickness) was used whereby a high-temperature column was applied.…”

Section: Chemical Composition Analysis By Gcmsmentioning

confidence: 99%

ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS IN MEDICINAL PLANT Centella asiatica AND STUDY THE EFFECTS ON FUNGAL ACTIVITIES

Bhuyar

Rahim

Maniam

et al. 2021

J microb biotech food sci

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Preparation of plant crude methanol extract Centella asiatica were purchased from the local retail market. All plant materials washed thoroughly under running tap water and air dried at room temperature for two days and ground into a fine powder. The air-dried and powdered plant material are extracted with 400 ml of 95.5% methanol, kept in the room temperature for 72 h with periodic stirring. Thereafter the extract was filtered by Medicinal plants are sources of important therapeutic aids for alleviating human ailments. The present research investigation was carried out to study anti-fungal activity of C. asiatica were tested against C. albicans, Aspergillus niger, and Penicillium sp. using two methods, disc diffusion method and broth dilution method. C. asiatica crude methanol extract was found to be the most effective against fungal activity. Compared to disc diffusion, broth dilution was a more appropriate method to quantitatively determine the anti-Candida activity of plant extract, whereby the MIC values of the crude extracts was determined. The result from disc diffusion assay demonstrates that plant extracts have an inhibitory effect. However, the broth dilution method result reveal that C. asiatica crude methanol extract has lower MIC values, meaning it has more prove that C. asiatica has an anti-fungal effect. Bioactive analysis results reveled that bioactive compounds present in the leaf, stems, roots, and the whole plant extract from Centella asiatica are the major chemical constituents are n-Hexadecanoic acid (99%), cis-Vaccenic acid (91%), 5-Hydroxymethylfurfural (88%), Tetradecanoic acid (86%). Further study is required to find out the specific phytochemical which is responsible for its medicinal value. ARTICLE INFO

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“…Thus, directing at the decreasing of the consumption of synthetic chemical compounds that may impair the consumer’s health and well‐being has created demand for natural antioxidants by consumers and the meat industry. In this manner, natural antioxidants from plants extract can be used as alternatives to synthetic antioxidants owing to their comparable or superior effect on the inhibition of lipid oxidation and bacterial growth (Bhuyar, Rahim, Sundararaju, Ramaraj, et al, 2020; Bhuyar et al., 2019; Fernandes et al., 2016). The use of natural antioxidants in the food industry was preferable as natural products do not necessitate comprehensive safety testing prior to use.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and antioxidative activity of the essential oil and seed extracts of Artocarpus heterophyllus for effective shelf‐life enhancement of stored meat

Ramli

Badrulzaman

Hamid

et al. 2020

J Food Process Preserv

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This study was designed to examine the in vitro antimicrobial and antioxidant activity of the essential oil and various extracts processed from Jackfruit seed (Artocarpus heterophyllus). Seed extracts obtained from Jackfruit were tested for TPC and TFC, and the TPC of 34.48 ± 1.77 mg GAE/g extract was detected while TFC of 0.19 ± 0.004 mg QE/g extract was found significantly. Phytochemical screening of A. heterophyllus seed extracts using UPLC–QTOF/MS revealed the presence of eight compounds, representing (−)‐Epiafzelechin‐3‐O‐(6″‐O‐acetyl)‐β‐D‐allosepyranoside was the most abundant phenolic compound. Further samples were also subjected to a screening for their possible antioxidant activity using 2,2‐diphenyl‐1‐picrylhydrazyl assay. DPPH analysis revealed that seed extract of A. heterophyllus has satisfactory radical scavenging capacity which could scavenge the free radicals produced during oxidation process in meat. The antioxidant activity can be observed for meat treated with seed extract as the TBA value was decreased in the samples on the addition of seed extract. However, in this study, seed extract treatment exhibited significant increase in MetMb content at 2 and 3 days of storage than in the control. The significance for all comparisons was determined at the p < .05 level. This result indicated that Jackfruit extract can be used commercially for meat shelf‐life management. Practical applications Jackfruit seed extract is a natural preservative which improves the shelf‐life storage of meat without causing any adverse effects. Natural preservative from plant source always better option when compare with chemical preservative. Jackfruit seed extracts could be used for shelf‐life enhancement of stored meat in natural condition without affecting the quality as well as the taste of meat.

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Microalgae cultivation using palm oil mill effluent as growth medium for lipid production with the effect of CO2 supply and light intensity

Cited by 80 publications

References 42 publications

Exploration of bioactive compounds and antibacterial activity of marine blue-green microalgae (Oscillatoria sp.) isolated from coastal region of west Malaysia

Exploration of bioactive compounds and antibacterial activity of marine blue-green microalgae (Oscillatoria sp.) isolated from coastal region of west Malaysia

ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS IN MEDICINAL PLANT Centella asiatica AND STUDY THE EFFECTS ON FUNGAL ACTIVITIES

Antibacterial and antioxidative activity of the essential oil and seed extracts of Artocarpus heterophyllus for effective shelf‐life enhancement of stored meat

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