Microalgae with artificial intelligence: A digitalized perspective on genetics, systems and products

Teng, Sin Yong; Yew, Guo Yong; Sukačová, Kateřina; Show, Pau Loke; Máša, Vítězslav; Chang, Jo Shu

doi:10.1016/j.biotechadv.2020.107631

Cited by 74 publications

(18 citation statements)

References 195 publications

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“…Artificial Intelligence (AI), which has recently acquired prominence in the field of microalgal research, can be used to make molecular predictions with respect to gene sequencing and editing. AI and microalgal informatics have the potential to improve current approaches to study genetic information while can also bridge the gap in microalgal engineering ( Yong et al, 2020 ).…”

Section: Future Perspectivesmentioning

confidence: 99%

“…Bioinformatic tools are efficiently used to identify these types of transfers by comparing homologues in the relevant genome sequences. Further, combining algal engineering with advanced technologies like Artificial Intelligence (AI) will help in prediction of molecular interactions and their products ( Yong et al, 2020 ). In addition, active metabolic profiling and comparison can also help in discovering the novel secondary metabolites of commercial importance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects

Sreenikethanam

Raj

Banu

et al. 2022

Front. Bioeng. Biotechnol.

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Microalgae are highly diverse photosynthetic organisms with higher growth rate and simple nutritional requirements. They are evolved with an efficiency to adapt to a wide range of environmental conditions, resulting in a variety of genetic diversity. Algae accounts for nearly half of global photosynthesis, which makes them a crucial player for CO2 sequestration. In addition, they have metabolic capacities to produce novel secondary metabolites of pharmaceutical, nutraceutical and industrial applications. Studies have explored the inherent metabolic capacities of microalgae with altered growth conditions for the production of primary and secondary metabolites. However, the production of the targeted metabolites at higher rates is not guaranteed just with the inherent genetic potentials. The strain improvement using genetic engineering is possible hope to overcome the conventional methods of culture condition improvements for metabolite synthesis. Although the advanced gene editing tools are available, the gene manipulation of microalgae remains relatively unexplored. Among the performed gene manipulations studies, most of them focus on primary metabolites with limited focus on secondary metabolite production. The targeted genes can be overexpressed to enhance the production of the desired metabolite or redesigning them using the synthetic biology. A mutant (KOR1) rich in carotenoid and lipid content was developed in a recent study employing mutational breeding in microalgae (Kato, Commun. Biol, 2021, 4, 450). There are lot of challenges in genetic engineering associated with large algal diversity but the numerous applications of secondary metabolites make this field of research very vital for the biotech industries. This review, summarise all the genetic engineering studies and their significance with respect to secondary metabolite production from microalgae. Further, current genetic engineering strategies, their limitations and future strategies are also discussed.

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Section: Future Perspectivesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects

Sreenikethanam

Raj

Banu

et al. 2022

Front. Bioeng. Biotechnol.

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“…Although some of these applications are currently in their infancy, they are being implemented more and more to explore new ways to improve shrimp efficiency and resistance to culture conditions. Therefore, the adaptation of technologies developed in related fields, as is the case of the culture of microalgae, 119 has the potential to represent a qualitative leap not only in our understanding of the shrimp production system, as a whole, but will also allow us to manipulate it with much greater precision. This will ultimately make possible to consolidate the sustainability of this important resource for the aquaculture industry.…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Shrimp immune response: A transcriptomic perspective

Fajardo

Martı́nez-Rodrı́guez

Costas

et al. 2021

Reviews in Aquaculture

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During the last 15 years, a significant amount of data related to the crustacean immune system has been generated, mainly led by the advances made in the field of transcriptomic analysis through massive mRNA sequencing technology. In order to shed new light on the genes and metabolic pathways associated with the immune system in shrimp species, we have carried out a meta‐analysis using the current literature and the Sequence Read Archive (SRA) database of the National Center for Biotechnology Information (NCBI), which has 1195 published transcriptomic data sets until 31 December 2020, from different tissues and 14 Penaeus species. The transcriptomic analyses published so far have explored the effects of the challenge from viral and bacterial pathogens, as well as abiotic stressors, such as ammonia, temperature, salinity, oxygen deprivation and pH. They have also explored the effect of dietary factors and beneficial microbes (pre‐ and probiotics) in the immune response. In order to harness the vast amount of information available, multidisciplinary efforts have to generate better genomic tools to overcome the complexity of the genome and to implement the knowledge gained into commercial applications for the improvement of the culture conditions, adaptation of more sustainable feeds and producing strains showing more resilience/tolerance to diseases.

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“…On the other hand, algae cultivation in fresh water and wastewater [9,10] is also popular. Currently, with the advent of the latest technology from industry 4.0 [11], the maximum benefit of extracting the oil from microalgae and converting it to biodiesel is made more economical with the latest optimization and artificial intelligence (AI) tools [12][13][14]].…”

Section: Introductionmentioning

confidence: 99%

Preparation and physicochemical properties of naturally grown green Spirogyra algae biodiesel

Kalyani

Prasad

Kolakoti³

2023

CI&CEQ

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In this study, biodiesel was produced from a naturally grown green algae (spirogyra). The algae were cultivated in an open pond for 180 days without any fertilizers or nutrients. The dried algae powder to oil yield and significant fuel properties of viscosity, density, cetane number, calorific value, flash point, pour and cloud points are investigated for B10 to B100 blends. The results of solvent oil extraction show that at a 1:2 (algae powder to solvent) ratio and 65oC, algae oil yield was observed as 22.66%. Furthermore, Box-Behnken assisted response surface optimization technique was implemented. From the 29 random experiments, 96.24% spirogyra algae oil biodiesel (SAOBD) yield was achieved under the optimum conditions of 50oC, 180 minutes, the molar ratio of 9:1 and catalyst concentration of 0.5 wt%. The fatty acid composition (FAC) by gas chromatography reveals that 73.95 wt% saturated FAC was observed in SAOBD. The significant fuel properties are measured by following ASTM-D6751 standards and 40% SAOBD in diesel fuel could possibly be an optimum blend ratio for engine experimentation. Finally, regression equations with high correlation coefficients (R2) were developed for the fuel properties to predict the various blend ratios.

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Microalgae with artificial intelligence: A digitalized perspective on genetics, systems and products

Cited by 74 publications

References 195 publications

Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects

Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects

Shrimp immune response: A transcriptomic perspective

Preparation and physicochemical properties of naturally grown green Spirogyra algae biodiesel

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