Microarray analysis and real-time PCR assay developed to find biomarkers for mercury-contaminated soil

Abstract

The evaluation of mercury (Hg) toxicity in agricultural soil is of great concern because its bioavailability and bioaccumulation in organisms through the food chain can have adverse effects on human health. Therefore, the aim of this study was to develop sensitive biomarkers for Hg stress in agricultural soil. With the results obtained from a high-throughput cDNA microarray, 12 Hg-responsive genes were selected to examine their concentration-dependent responses to Hg stress at different Hg concentrations. The lowest observable adverse effect concentrations (LOAECs) of Hg were 0.8 mg kg for seed germination, 1.6 mg kg for root biomass, 0.8 mg kg for root elongation, and 0.8 mg kg for root morphology, respectively, whereas the lowest Hg treatments (0.1-0.4 mg kg) could generally induce differential expression of genes. These results indicated that the detection of Hg in soil at the molecular level is a highly sensitive method. Moreover, the Hg soil content exhibited a significant positive correlation with the relative expression of probable glutathione -transferase parA ( = 0.637, = 0.05), chlorophyll a-b binding protein 13, chloroplastic-like ( = 0.689, = 0.05) and geranylgeranyl pyrophosphate synthase 1 ( = 0.682, = 0.05), implying that the three genes are good candidates to detect Hg-contaminated soil.

Toxic Effects and Molecular Mechanism of Different Types of Silver Nanoparticles to the Aquatic Crustacean Daphnia magna

“…With the rapid development of high-throughput sequencing technologies, the possible physiological functions in specific tissues and organs have explored fully at the transcriptomic level. − RNA sequencing (RNA-Seq) has proven to be a rapid and powerful technique, with the low noise, high efficiency, and massive data output enabling high-throughput sequencing of the whole transcriptome . RNA-Seq can aid in identifying biomarkers of exposure, differentiating toxicity between AgNPs, detecting casual contaminants in environmental samples, and assessing the mechanisms of response to stressors. , Although RNA-Seq has been performed in aquatic species such as Pandalus latirostris , Salmo salar , Oryzias melastigma , Labeo rohita , Lateolabrax japonicas , Litopenaeus vannamei , Ostrea edulis , Pinctada fucata , and Patella vulgate , the molecular mechanisms underlying the effects of different types of AgNPs on aquatic organisms are still rare.…”

Comparative response of SOD in different plants against cadmium and drought stress at the molecular level

“…Expression profiles of SOD genes related to Cd and drought stress individually in A. thaliana, S. lycopersicum, T. aestivum and O. sativa were investigated using the genome microarray data from the NCBI and GEO database. Generally, hydroponic or soil culture were carried out in these experiments, and the exposure concentrations and exposure duration of these experiments were described in the literatures (Sultana et al, 2016;Dixit et al, 2015;Park et al, 2012;Peremarti et al, 2014;Ibort et al, 2018;Lau et al, 2018;Li et al, 2016;Kusunoki et al, 2018;Fischer et al, 2017;Dubey et al, 2016;Cheng et al, 2018;Cui et al, 2016). The accession numbers identified in the GEO database included drought stress (GSE76827, GSE78504, GSE32065, GSE42214, GSE47090, GSE106317, GSE8161), Cd stress (GSE22114, GSE108751, GSE94314, GSE25206, GSE63024, GSE132104) ( Table 1).…”

“…Microarray analysis and real-time PCR assay developed to find biomarkers for mercurycontaminated soil. GSE63024 Cui et al, 2016 02g434000, Traescs7a02g048600, Traescs7a02g090400, Traescs2b02g567600, Traescs2d02g123300, Traescs2d02g-538300, Traescs7d02g043000, Traescs7d02g086400 and Traescs7d02g290700), and 9 (Os03g0351500, Os03g02192-00, Os08g0561700, Os04g0573200, Os07g0665200, Os05g-0323900 and Os06g0115400) recognized as SOD isogenes obtained from S. lycopersicum, T. aestivum and O. sativa.…”

Inorganic Hg toxicity in plants: A comparison of different genotoxic parameters

“…Due to its bioavailability and bioaccumulation in organisms through the food chain, Hg contamination of agricultural soils is of great concern. However, and contrarily to animal cells, its cyto and genotoxic effects remain to unveil, allied to the urgent need to develop sensitive biomarkers as highlighted by Hou et al, (2016).…”