Microarray analysis of di-n-butyl phthalate and 17α ethinyl-oestradiol responses in three-spined stickleback testes reveals novel candidate genes for endocrine disruption

Prokkola, Jenni M.; Katsiadaki, Ioanna; Sebire, Marion; Elphinstone-Davis, Jessica; Pausio, Sanna; Nikinmaa, Mikko; Leder, Erica H.

doi:10.1016/j.ecoenv.2015.09.039

Cited by 11 publications

(3 citation statements)

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“…Experimental exposure to EE2 is associated with significant down- and up-regulation of various physiological pathways in fish. For example, juvenile sticklebacks ( Gasterosteus aculeatus ) exposed to 35–40 ng/L of EE2 showed reduced expression in testis for genes related to steroid biosynthesis (e.g., cytochrome P450 11A1 and 17A1, 3 beta- and steroid delta-isomerase 2) and increased expression in genes associated to epidermal growth (e.g., epidermal growth factor receptor) and xenobiotic metabolism (e.g., fms-related tyrosine kinase 4) [16, 17]. Juvenile coho salmon ( Oncorhynchus kisutch ) exposed to 2 or 10 ng/L of EE2 showed altered expression of genes linked to sexual-development and reproductive function: hepatic vitellogenin and pituitary luteinizing hormone were up-regulated, while pituitary follicle-stimulating hormone was down-regulated [16, 17].…”

Section: Introductionmentioning

confidence: 99%

Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae)

et al. 2019

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The synthetic 17α-ethinylestradiol (EE2) is a common estrogenic pollutant that has been suspected to affect the demography of river-dwelling salmonids. One possibility is that exposure to EE2 tips the balance during initial steps of sex differentiation, so that male genotypes show female-specific gene expression and gonad formation. Here we study EE2 effects on gene expression around the onset of sex differentiation in a population of European grayling ( Thymallus thymallus ) that suffers from sex ratio distortions. We exposed singly-raised embryos to one dose of 1 ng/L EE2, studied gene expression 10 days before hatching, at the day of hatching, and around the end of the yolk-sac stage, and related it to genetic sex (sdY genotype). We found that exposure to EE2 affects expression of a large number of genes, especially around hatching. These effects were strongly sex-dependent. We then raised fish for several months after hatching and found no evidence of sex reversal in the EE2-exposed fish. We conclude that ecologically relevant (i.e. low) levels of EE2 pollution do not cause sex reversal by simply tipping the balance at early stages of sex differentiation, but that they interfere with sex-specific gene expression. Electronic supplementary material The online version of this article (10.1186/s12864-019-5955-z) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae)

et al. 2019

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“…Thus, the present study aimed to better understand the impact of three different BPs (BPA, BPS, BPF), on oxidative stress, metabolic detoxification and innate immune responses, in adult three-spined stickleback (Gasterosteus aculeatus), a species often used to investigate the effects of EDCs (Bado-Nilles et al 2014, Jolly et al 2009, Porseryd et al 2019, Prokkola et al 2016. Two types of exposure scenario (21-day exposure and seven-day exposure followed by seven-day depuration) were performed to better understand the physiological responses of sticklebacks.…”

Section: Introductionmentioning

confidence: 99%

Exposure and hazard of bisphenol A, S and F: a multi-biomarker approach in three-spined stickleback

Mit

Beaudouin

Palluel

et al. 2023

Environ Sci Pollut Res

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Due to the oestrogenic behaviour of bisphenol (BP) A, industries have developed many substitutes, such as BPS and BPF. However, due to their structural similarities, adverse effects on reproduction are currently observed in various organisms, including fish. Even if new results have shown impacts of these bisphenols on many other physiological functions, their mode of action remains unclear. In this context, we proposed to better understand the impact of BPA, BPS and BPF on immune responses (leucocyte sub-populations, cell death, respiratory burst, lysosomal presence and phagocytic activity) and on biomarkers of metabolic detoxification (ethoxyresorufin-O-deethylase, EROD, and glutathione S-transferase, GST) and oxidative stress (glutathione peroxidase, GPx, and lipid peroxidation with thiobarbituric acid reactive substance method, TBARS) in an adult sentinel fish species, the threespined stickleback. In order to enhance our understanding of how biomarkers change over time, it is essential to determine the internal concentration responsible for the observed responses. Therefore, it is necessary to explore the toxicokinetics of bisphenols. Thus, sticklebacks were exposed either to 100 µg/L of BPA, BPF or BPS for 21 days, or for seven days to 10 and 100 µg/L of BPA or BPS followed by seven days of depuration. Although BPS has very different TK, due to its lower bioaccumulation compared to BPA and BPF, BPS affect oxidative stress and phagocytic activity in the same way. For those reasons, the replacement of BPA by any substitute should be made carefully in terms of risk assessment on aquatic ecosystems.

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“…Experimental exposure to EE2, for example of juvenile sticklebacks ( Gasterosteus aculeatus ) to 35-40 ng/L, or of juvenile coho salmon ( Oncorhynchus kisutch ) to 2 or 10 ng/L, are associated with significant down- and up-regulations of various physiological pathways [14, 15]. Some of these effects on gene expression may be linked to the toxic effects of EE2 observed in juveniles and adults.…”

Section: Introductionmentioning

confidence: 99%

Sex-specific changes in gene expression and delayed sex differentiation in response to estrogen pollution in grayling (Salmonidae)

Selmoni

Maitre

Roux

et al. 2017

Preprint

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26The synthetic 17α-ethinylestradiol (EE2) is an estrogenic compound of oral contraceptives 27 and therefore a common pollutant that has been suspected to affect the demography of river-28 dwelling salmonids. We study a population of European grayling (Thymallus thymallus) that 29 suffers from sex ratio distortions. Here we test how ecologically relevant concentrations of 30 EE2 affect sex-specific gene expression around early stages of sex differentiation. We 31 collected gametes from F1s of wild spawners, used them for in vitro fertilizations, and raised 32 the resulting embryos singly under experimentally controlled conditions. Embryos were either 33 exposed to 1ng/L EE2 or sham-exposed. RNA was collected from samples taken 10 days 34 before hatching, at the day of hatching, and towards the end of the yolk-sac stage, to study 35 gene expression and relate it to genetic sex (sdY genotype). We found that EE2 affects gene 36 expression of a very large number of genes especially at the day of hatching. The effects of 37 EE2 on gene expression is strongly sex-specific. At the day of hatching, EE2 affected about 38 twice as many genes in females than in males, and towards the end of the yolk-sac larval 39 stage, EE2 effects were nearly exclusively observed in females. Among the many effects was, 40 for example, a surprising EE2-induced molecular masculinization in the females' heads. 41Histological examination of gonadal development of EE2-treated or sham-exposed juveniles 42 during the first 4.5 months after hatching revealed a delaying effect of EE2 on sex 43 differentiation. Because grayling sex determination goes through an all-male stage (a rare 44 case of undifferentiated gonochorism), the rate of EE2-induced sex reversal could not be 45 unequivocally determined during the observational period. However, two EE2-treated genetic 46 males had ovarian tissues at the end of the study. We conclude that common levels of EE2 47 pollution affect grayling from very early stages on by interfering with male and female gene 48 expression around the onset of sex differentiation, by delaying sex differentiation, and by 49 feminizing some males. 50 51All rights reserved. No reuse allowed without permission.was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

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Microarray analysis of di-n-butyl phthalate and 17α ethinyl-oestradiol responses in three-spined stickleback testes reveals novel candidate genes for endocrine disruption

Cited by 11 publications

References 77 publications

Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae)

Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae)

Exposure and hazard of bisphenol A, S and F: a multi-biomarker approach in three-spined stickleback

Sex-specific changes in gene expression and delayed sex differentiation in response to estrogen pollution in grayling (Salmonidae)

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