Microarray analysis of gene expression in eastern oyster (Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo (Perkinsus marinus) challenge

Wang, Shaolin; Peatman, Eric; Lv, Hong; Bushek, David; Ford, Susan E.; Kucuktas, Huseyin; Quilang, Jonas P.; Li, Ping; Wallace, Richard K.; Wang, Yongping

doi:10.1016/j.fsi.2010.07.035

Cited by 60 publications

(45 citation statements)

References 67 publications

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“…This late suppression is in accordance with that observed in the present model of long term exposure to the myxosporean. Transcriptional studies have also demonstrated this gene suppression effect in other species, as in molluscs infected by the metazoan parasite Echinostoma paraensei, which had many down-regulated transcripts with potential immune functions (Adema et al, 2010), or in oysters chronically infected by the protozoan parasite Perkinus marinus (Wang et al, 2010).…”

Section: Discussionmentioning

confidence: 94%

Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

Davey¹,

Calduch-Giner²,

Houeix³

et al. 2011

Molecular Immunology

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Section: Discussionmentioning

confidence: 94%

Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

Davey¹,

Calduch-Giner²,

Houeix³

et al. 2011

Molecular Immunology

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“…Oysters susceptible to summer mortalities exhibit a higher level of ROS compared to that of resistant animals before mortality event, thus linking mortality with the antioxidant disorder of the sensitive oysters Samain et al 2007). Recent study using microarray analysis demonstrated the activation of antioxidant processes after Perkinsus marinus challenge in C. virginica (Wang et al 2010). Therefore, we hypothesize that the estimation of the antioxidant enzyme activities is a useful tool to monitor the oxidative stress associated with massive mortality emergence.…”

Section: Introductionmentioning

confidence: 90%

“…This implies another origin of ROS production, for example, a possible increase of ROS associated with the immune response. In bivalves, ROS production during infection have been suggested to be a host defense mechanism to counter attacks of pathogenic bacteria (Canesi et al 2002;Lambert et al 2003;Labreuche et al 2006b;Wang et al 2010). The increase of CAT, GPX and SOD protein-specific activity shows that moribund larvae increased the proportion of antioxidant enzymes in their tissues, thus pinpointing the importance of antioxidant defenses during the massive mortality event.…”

Section: Characterization Of the Massive Mortalitymentioning

confidence: 99%

Physiological and biochemical changes associated with massive mortality events occurring in larvae of American oyster (Crassostrea virginica)

Genard

Lemarchand

Boudry

et al. 2011

Aquat. Living Resour.

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-In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of some lipid classes (free fatty acids, diglycerides, and acetone mobile phospholipids); (4) lower levels of phospholipids and protein; (5) higher contents of non-methylene interrupted dienoic fatty acids (22:2 NMI); (6) a decrease in energy metabolism activity (citrate synthase and cytochrome oxidase activities); (7) a higher oxidative stress (lipid peroxidation level); and (8) an activation of antioxidant defences before mortality (glutathione peroxidase and superoxide dismutase). We hypothesized that mortality emergence was related to higher energy consumption coupled with the progressive decline in feeding activity, lowered energy reserves and a decrease in energy metabolism activity. Thus, the low energy availability limited the efficiency of antioxidant defenses, resulting in a higher oxidative stress.

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“…Crassostrea virginica galectins, a family of lectins that bind to b-galactosides, selectively recognize P. marinus that may use galectin to gain entry to host cells [139,140]. The downregulation of galectin after P. marinus infection [141] may represent an attempt of the host to limit infections. Other sugar-binding PRRs that may participate in immune responses include fibrinogen-related proteins (FREPs), peptidoglycan recognition proteins (PGRPs) and Gram-negative binding proteins (GNBPs).…”

Section: (C) Lectins and Other Carbohydrate Pattern Recognition Recepmentioning

confidence: 99%

Infectious diseases of marine molluscs and host responses as revealed by genomic tools

Guo¹,

Ford²

2016

Phil. Trans. R. Soc. B

Self Cite

124

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More and more infectious diseases affect marine molluscs. Some diseases have impacted commercial species including MSX and Dermo of the eastern oyster, QPX of hard clams, withering syndrome of abalone and ostreid herpesvirus 1 (OsHV-1) infections of many molluscs. Although the exact transmission mechanisms are not well understood, human activities and associated environmental changes often correlate with increased disease prevalence. For instance, hatcheries and large-scale aquaculture create high host densities, which, along with increasing ocean temperature, might have contributed to OsHV-1 epizootics in scallops and oysters. A key to understanding linkages between the environment and disease is to understand how the environment affects the host immune system. Although we might be tempted to downplay the role of immunity in invertebrates, recent advances in genomics have provided insights into host and parasite genomes and revealed surprisingly sophisticated innate immune systems in molluscs. All major innate immune pathways are found in molluscs with many immune receptors, regulators and effectors expanded. The expanded gene families provide great diversity and complexity in innate immune response, which may be key to mollusc's defence against diverse pathogens in the absence of adaptive immunity. Further advances in host and parasite genomics should improve our understanding of genetic variation in parasite virulence and host disease resistance.

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Microarray analysis of gene expression in eastern oyster (Crassostrea virginica) reveals a novel combination of antimicrobial and oxidative stress host responses after dermo (Perkinsus marinus) challenge

Cited by 60 publications

References 67 publications

Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

Physiological and biochemical changes associated with massive mortality events occurring in larvae of American oyster (Crassostrea virginica)

Infectious diseases of marine molluscs and host responses as revealed by genomic tools

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