Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid

Motojima, Hideko; Villareal, Myra O.; Han, Jianzhong; Isoda, Hiroko

doi:10.1007/s10616-010-9333-6

Cited by 14 publications

(10 citation statements)

References 25 publications

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“…Fulvic acid (FA), a class of compounds of humic substances, is a mixture of polyphenolic acid compounds formed through the degradation of organic substances such as dead plants, microbes and animals by chemical and biological processes [ 1 ]. FA has been reported recently to have nutraceutical properties and physiological action on the human body.…”

Section: Introductionmentioning

confidence: 99%

Fulvic acid attenuates homocysteine-induced cyclooxygenase-2 expression in human monocytes

Chien

Chen

Kuo

et al. 2015

BMC Complement Altern Med

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BackgroundHomocysteine and pro-inflammatory mediators such as cyclooxygenase-2 (COX-2) have been linked to vascular dysfunction and risks of cardiovascular diseases. Fulvic acid (FA), a class of compounds of humic substances, possesses various pharmacological properties. However, the effect of FA on inflammatory responses of the monocytes remains unclear. We investigated the regulatory effect of FA on homocysteine-induced COX-2 expression in human monocytes.MethodsPeripheral blood monocytes and U937 cells were used for all experiments. Real-time PCR and ELISA assay were used to analyze the COX-2 mRNA expression and PGE2 secretion, respectively. Specific inhibitors were used to investigate the mechanism of homocysteine-mediating COX-2 mRNA expression and PGE2 secretion. Luciferase assay, transcription factor ELISA, and chromatin immunoprecipitation were used to determine the role of nuclear factor-κB in FA-mediated inhibition of homocysteine effect on monocytes.ResultsThe results show that pretreating monocytes with FA inhibited the homocysteine-induced COX-2 expression in a dose-dependent manner. Stimulation of U937 monocytes with homocysteine induced rapid increases in the phosphorylation of ERK and JNK; the inhibitor for ERK and JNK attenuated the homocysteine-induced nuclear factor-κB activation and COX-2 expression. Transcription factor ELISA and chromatin immunoprecipitation assays showed that FA blocked the homocysteine-induced increases in the binding activity and in vivo promoter binding of nuclear factor-κB in monocytes.ConclusionsOur findings provide a molecular mechanism by which FA inhibits homocysteine-induced COX-2 expression in monocytes, and a basis for using FA in pharmaceutical therapy against inflammation.

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Section: Introductionmentioning

confidence: 99%

Fulvic acid attenuates homocysteine-induced cyclooxygenase-2 expression in human monocytes

Chien

Chen

Kuo

et al. 2015

BMC Complement Altern Med

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“…Shilajit is one of the substances most used in Ayurvedic medicine for its therapeutic antioxidant and anti-inflammatory properties, as well as its alleged antioxidant and immunomodulatory effects[4]. There has been only one in vitro study where fulvic acid has shown an inhibitory effect on human leukemia basophil (KU812) cells[6]. The active constituent of Shilajit are dibenzo-α-pyrones and related metabolites and fulvic acid.…”

Section: Discussionmentioning

confidence: 99%

Exercise-induced anaphylaxis with an Ayurvedic drug as cofactor: A case report

Losa

Deidda

Firinu

et al. 2019

WJCC

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BACKGROUND The practice of Indian Ayurvedic medicine is spreading in Western countries and Shilajit is one of the most used drugs, for its antioxidant activities and immunomodulatory effects. Albeit Shilajit has showed a high degree of safety, it can act as cofactor of anaphylaxis, especially in condition at high risk, such as mast cell activation syndrome (MCAS). We reported this case to sensitize practitioners to investigate to the use of complementary and alternative medicine, in case of exercise-induced anaphylaxis (EIAn). CASE SUMMARY A 43-year-old woman, working as a dance teacher, developed urticaria after ingestion of rice, tuna and Shilajit, which did not respond to intramuscular corticosteroids. Subsequently, she developed dyspnoea and hypotension with loss of consciousness that arose 1 h after sexual activity. The patient did not refer personal history of atopy. Specific IgE for main food allergens resulted negative, with total IgE levels of 14 IU/L. Oral provocation test with Shilajit was not perfomed because the patient refused, but we performed prick-by-prick and patch test that resulted negative. Serum tryptase at the time of anaphylaxis was 20.6 μg/L that fell down to of 10.6 μg/L after therapy, but has remained at the high value after two days and during the follow-up. We performed an analysis of th e c-KIT gene in peripheral blood, which was negative. We felt the diagnosis consistent with EIAn in a patient with a possible MCAS. CONCLUSION In Western countries the use of drugs from Ayurvedic medicine is more common than in the past. These substances can be cofactors of anaphylaxis in patients with risk factors.

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“…With an increase in FA concentration from 30 μg/mL, the H 2 O 2 scavenging activity began to decline, and the H 2 O 2 scavenging activity at the highest concentration of 100μg mL -1 decreased to 25.78%. FA, a soluble class of humic substances at each pH value, is a mixture of polyphenolic acid compounds that occur as a result of chemical and biological degradation of organic materials such as dead plants, microbes and animals (Motojima et al, 2011;Guzman-Martinez et al, 2013). There are studies to investigating antioxidant activity of FA obtained from different natural sources e.g.…”

Section: H 2 O 2 Scavenging Activitymentioning

confidence: 99%

Investigation of Antioxidant and In Vitro Wound Healing Activity of Fulvic Acid

Aslantürk

Çelik

Sönmez³

2019

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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This study aimed to investigate the antioxidant and in vitro wound healing activities of fulvic acid (FA) purified from Hüsamlar leonardite in Muğla/Turkey. Purified FA was analyzed with FT/IR spectrophotometer and flame photometer for determining functional groups and cation impurities, respectively. 1, 5, 10, 20, 30, 50, 100 µg mL-1 concentrations of FA were tested for antioxidant activity (by using DPPH radical scavenging and H 2 O 2 scavenging assays). Also, effect of FA on BJ human foreskin fibroblast and HaCaT spontaneously immortalized non-tumorigenic human keratinocyte cells proliferation was tested by in vitro MTT and WST-8 assays and on cell migration by wound healing assay (scratch assay). The results of the study show that the FA has a low DPPH radical scavenging activity, but it exhibited high H 2 O 2 scavenging activity at low concentrations. The effect of FA on the proliferation of BJ and HaCaT cells varied according to the cell type, FA concentration and treatment time. However, it accelerated wound healing by increasing cell migration, especially in HaCaT cells. 1 and 10 µg mL-1 FA had a significant wound healing effect on BJ cells, and all concentrations of FA had a significant wound healing effect on HaCaT cells at the end of the 24 h treatments.

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Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid

Cited by 14 publications

References 25 publications

Fulvic acid attenuates homocysteine-induced cyclooxygenase-2 expression in human monocytes

Fulvic acid attenuates homocysteine-induced cyclooxygenase-2 expression in human monocytes

Exercise-induced anaphylaxis with an Ayurvedic drug as cofactor: A case report

Investigation of Antioxidant and In Vitro Wound Healing Activity of Fulvic Acid

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