Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer

Solmi, Rossella; Ugolini, Giampaolo; Rosati, Giancarlo; Zanotti, Simone; Lauriola, Mattia; Montroni, Isacco; Governatore, Marco Del; Caira, A; Taffurelli, Mario; Santini, Donatella; Coppola, Domenico; Guidotti, Lia; Carinci, Paolo; Strippoli, Pierluigi

doi:10.1186/1471-2407-6-250

Cited by 35 publications

(33 citation statements)

References 41 publications

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“…GAS is expressed in normal gastric mucosa but not in normal colon. GAS levels, however, have been increased in colon cancer (51). Other genes that were up-regulated in both ovarian and colon cancer included MMP1, SERPINE1, REG4, TFF1, IL6, and IL8.…”

Section: Discussionmentioning

confidence: 99%

Patterns of Gene Expression in Different Histotypes of Epithelial Ovarian Cancer Correlate with Those in Normal Fallopian Tube, Endometrium, and Colon

Marquez

Baggerly

Patterson

et al. 2005

Clinical Cancer Research

282

203

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Purpose: Epithelial ovarian cancers are thought to arise from flattened epithelial cells that cover the ovarian surface or that line inclusion cysts. During malignant transformation, different histotypes arise that resemble epithelial cells from normal fallopian tube, endometrium, and intestine. This study compares gene expression in serous, endometrioid, clear cell, and mucinous ovarian cancers with that in the normal tissues that they resemble. Experimental Design: Expression of 63,000 probe sets was measured in 50 ovarian cancers, in 5 pools of normal ovarian epithelial brushings, and in mucosal scrapings from 4 normal fallopian tube, 5 endometrium, and 4 colon specimens. Using rank-sum analysis, genes whose expressions best differentiated the ovarian cancer histotypes and normal ovarian epithelium were used to determine whether a correlation based on gene expression existed between ovarian cancer histotypes and the normal tissues they resemble. Results: When compared with normal ovarian epithelial brushings, alterations in serous tumors correlated with those in normal fallopian tube (P = 0.0042) but not in other normal tissues. Similarly, mucinous cancers correlated with those in normal colonic mucosa (P = 0.0003), and both endometrioid and clear cell histotypes correlated with changes in normal endometrium (P = 0.0172 and 0.0002, respectively). Mucinous cancers displayed the greatest number of alterations in gene expression when compared with normal ovarian epithelial cells. Conclusion: Studies at a molecular level show distinct expression profiles of different histologies of ovarian cancer and support the long-held belief that histotypes of ovarian cancers come to resemble normal fallopian tube, endometrial, and colonic epithelium. Several potential molecular markers for mucinous ovarian cancers have been identified. Most investigators believe that epithelial ovarian cancersdevelop from a single layer of cells that cover the ovary or that line inclusion cysts immediately beneath the ovarian surface (1). Despite their origin from flattened cells without distinctive features, ovarian cancers differentiate during malignant transformation into four major histotypes: serous, endometrioid, clear cell, and mucinous. Pathologists have pointed to a morphologic resemblance between these histotypes and the differentiation of normal mucosal cells in the gynecologic and intestinal tracts. Thus, serous carcinomas are thought to resemble fallopian tube epithelium, endometrioid carcinomas to normal endometrium, clear cell carcinomas to vaginal rests, and mucinous carcinomas to normal endocervical glands or intestinal mucosa. Molecular alterations that contribute to these morphologic changes have not been adequately studied.Previous reports have shown that tumors of different histotypes can have distinct characteristics with regard to epidemiology, genetic abnormalities, expression of tumor markers, and response to chemotherapy (1 -3). Parity, breastfeeding, and oral contraceptive use have been associated with dec...

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Section: Discussionmentioning

confidence: 99%

Patterns of Gene Expression in Different Histotypes of Epithelial Ovarian Cancer Correlate with Those in Normal Fallopian Tube, Endometrium, and Colon

Marquez

Baggerly

Patterson

et al. 2005

Clinical Cancer Research

282

203

View full text Add to dashboard Cite

show abstract

“…Illegitimate transcription in blood cells is a well-known limitation and lowers the specificity of the RT-PCR analysis. While using RT-PCR in CTC detection could overcome the problems of lack of sensitivity associated with other methods, the selection of epithelial-specific mRNA is difficult (Solmi et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of MUC1, CK20, and hTERT expression in peripheral blood of gastrointestinal cancer patients in search of diagnostic criteria

Küçükyıldırım¹,

Erdem²,

Saglar³

et al. 2014

Turk J Biol

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The goal of this study was to identify the optimal marker or marker combinations for detection of gastrointestinal malignancies using reverse-transcriptase polymerase chain (RT-PCR) reaction. To detect the presence of tumors, we analyzed mucin 1 (MUC1), cytokeratin 19 (CK19), cytokeratin 20 (CK20), and human telomerase reverse transcriptase (hTERT) mRNA in the peripheral blood of 31 patients with gastrointestinal (esophagus, stomach, and colorectal) cancer and 30 healthy individuals. In RT-PCR analysis of the peripheral blood, 77.4% (24/31), 61.29% (19/31), and 45.16% (14/31) of cancer patients were positive for MUC1, CK20, and hTERT mRNA, respectively. According to our results, any one of these mRNA markers is a predictor of the presence of gastrointestinal tumors (P < 0.001) and colorectal tumors (P < 0.05). However, they did not have predictive potential for presence of metastasis in gastrointestinal tumors. As a result, combination of these 3 tumor-specific mRNA markers would increase the detection rate and may be clinically helpful in predicting tumor presence.

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“…Genomic DNA was isolated from 2 ml of peripheral blood anticoagulated with eDTA as previously described (22). DNA was extracted with the QIAamp DNA blood kit (Qiagen), according to the manufacturer's protocols.…”

Section: Methodsmentioning

confidence: 99%

IL23R, NOD2/CARD15, ATG16L1 and PHOX2B polymorphisms in a group of patients with Crohn's disease and correlation with sub-phenotypes

Lauriola

Ugolini²,

Rivetti³

et al. 2011

Int J Mol Med

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Abstract. Recent genomic research has identified interleukin-23 receptor (IL23R), nucleotide-binding oligomerization domain containing 2 caspase-activation recruitment domain 15 (NOD2/CARD15), autophagy related 16-like 1 (ATG16L1) and paired-like homeobox 2b (PHOX2B) as susceptibility loci for Crohn's Disease (CD). Our aim was to investigate these gene variants in a group of CD patients and to analyse the correlation to sub-phenotypes such as gender, smoking habits, disease behaviour at diagnosis, severity of disease and extra-intestinal manifestations. Nineteen patients with CD and 20 healthy controls were included in the study. The gene variants IL23R rs7517847 and rs11209026, NOD2/CARD15 rs2066845, PHOX2B rs16853571, ATG16L1 rs2241879 and rs2241880 were genotyped by PCR followed by sequencing. The frequency of the G risk allele of IL23R rs7517847 was found to be increased in patients with CD (42%) compared to that in control subjects (20%) [odds ratio (OR), 2.9; 95% confidence interval [CI], 1.06-7.9; P=0.03]. In addition, the homozygous condition GG was also associated with CD (OR, 8.70; 95% CI, 0.9-81.6; P=0.038). The analysis of correlation of genotype to sub-phenotypes showed an association of ATG16L1 rs2241879 with the lack of extra-intestinal manifestations (OR, 0.03; 95% CI, 0.002-0.45; P=0.006), and the patients defined as non-smokers displayed an increased frequency of the risk allele C (P=0.03). The present study confirms the association of the heterozygous and homozygous IL23R rs7517847 variant with CD and suggests an additive effect of smoking to the ATG16L1 rs2241879 C risk allele SNP, in the context of the multifactorial model established for the development of CD and a protective effect of the same allele against extra-intestinal manifestations.

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Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer

Cited by 35 publications

References 41 publications

Patterns of Gene Expression in Different Histotypes of Epithelial Ovarian Cancer Correlate with Those in Normal Fallopian Tube, Endometrium, and Colon

Patterns of Gene Expression in Different Histotypes of Epithelial Ovarian Cancer Correlate with Those in Normal Fallopian Tube, Endometrium, and Colon

Evaluation of MUC1, CK20, and hTERT expression in peripheral blood of gastrointestinal cancer patients in search of diagnostic criteria

IL23R, NOD2/CARD15, ATG16L1 and PHOX2B polymorphisms in a group of patients with Crohn's disease and correlation with sub-phenotypes

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