Microarray Evaluation of Age-related Changes in Human Dental Pulp

Tranasi, M.; Sberna, Maria Teresa; Zizzari, Vincenzo Luca; D'Apolito, G; Mastrangelo, Filiberto; Salini, L; Stuppia, Liborio; Tetè, Stefano

doi:10.1016/j.joen.2009.05.026

Cited by 39 publications

(25 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, the KEGG analysis also showed that the functional pathways that were enriched in the genes were primarily associated with cell and tissue proliferation, differentiation, and DNA repair. These results were in agreement with a previous study that compared the gene expression profiles of human dental pulp in young and older subjects by a microarray analysis [Tranasi et al, 2009].…”

Section: Discussionsupporting

confidence: 93%

Analysis of Senescence-Related Differentiation Potentials and Gene Expression Profiles in Human Dental Pulp Stem Cells

Qiao¹,

Liu²,

Yan³

et al. 2016

Cells Tissues Organs

View full text Add to dashboard Cite

Introduction: Dental pulp stem cell (DPSC)-mediated dental pulp regeneration is considered a promising method for the treatment of deep caries with pulpitis. However, mesenchymal stem cell (MSC) senescence is an adverse factor from the perspective of cell-based therapies. In this study, we investigated the characteristics and expression profiles of DPSCs from young and old donors. Methods: DPSCs from young and old donors were cultured in differentiation medium, and their differentiation potentials were assessed. Long noncoding RNA (LncRNA) microarray assays and a bioinformatic analysis were performed to investigate differences in LncRNA and mRNA expression profiles between DPSCs from young and old donors. Results: We found that DPSCs from young donors exhibited more powerful proliferation ability and greater osteogenic and adipogenic differentiation potentials than DPSCs from old donors. In DPSCs from young donors, numerous LncRNAs were significantly up- (n = 389) or down-regulated (n = 172) compared to DPSCs from old donors. Furthermore, 304 mRNAs were differentially expressed, including 247 up-regulated genes and 57 down-regulated genes in DPSCs from young donors. The bioinformatic analysis identified that several pathways may be associated with DPSC characteristics, such as those involved in the cell cycle and RNA transport, and revealed nuclear transcription factor Y subunit β, general transcription factor IIB, and nuclear receptor subfamily 3 group C member 1 as core regulatory factors and FR249114, FR299091, and ENST00000450004 as core LncRNAs. Conclusions: Our results indicated that senescence impaired the proliferation and differentiation potentials of DPSCs and that donor age is an important factor that affects their use for tooth regeneration. We also provide insight into the mechanisms responsible for senescence in DPSCs.

show abstract

Section: Discussionsupporting

confidence: 93%

Analysis of Senescence-Related Differentiation Potentials and Gene Expression Profiles in Human Dental Pulp Stem Cells

Qiao¹,

Liu²,

Yan³

et al. 2016

Cells Tissues Organs

View full text Add to dashboard Cite

show abstract

“…This finding may be associated with age-related changes in the morphophysiological characteristics of the dentin-pulp complex, combined with a decrease in pulpal blood flow. Tranasi et al (21) observed that the genetic changes underlie the histological modification of the dental pulp in aging. The authors reported that a significant reduction of the vitality in older dental pulp results from the gene expression, cellular and tissue development, cellular growth and proliferation charts.…”

Section: Discussionmentioning

confidence: 99%

Oxygen Saturation in the Dental Pulp of Maxillary Premolars in Different Age Groups - Part 1

et al. 2017

View full text Add to dashboard Cite

The aim of this study was to determine oxygen saturation levels in the dental pulp of maxillary premolars in different age groups. A total of 120 human maxillary premolars with normal dental pulps were selected covering the following age groups: 20-24, 25-29, 30-34, 35-39 and 40-44 years (n=24 each group). Oxygen saturation was assessed using pulse oximetry. Analysis of variance was used to assess differences in oxygen saturation levels and Tukey's test was used to identify the age groups that differed from each other. Significance was set at 0.05. Mean oxygen saturation of 120 premolars was 86.20% considering all age groups. Significantly reduced levels were found in the oldest group compared to the other groups: 40 to 44 years -80.00% vs. 89.71, 87.67, 88.71, and 84.80% for age groups 20-24, 25-29, 30-34, 35-39 years, respectively. The mean oxygen saturation levels were similar between 20 and 39 years of age (86.20%) in the whole sample, but reduced significantly in the 40-44-year age group, suggesting that older patients present lower oxygen saturation results even in the absence of pulp tissue injury.

show abstract

“…The distinct function of CD29 was associated with β1-integrin signaling and regulatory cell adhesion by interacting with the extracellular matrix (ECM), whereas integrin signaling has been shown to be of functional relevance for neural crest (33) as well as mesenchymal development (34,35). Tranasi et al (26) …”

Section: Discussionmentioning

confidence: 99%

“…identified several differentially expressed genes that were categorized as growth factors, transcription regulators, apoptosis regulators and genes of the ECM using a high-throughput microarray method, and indicated that in dental pulp from young individuals, biological functions of cells, including tissue differentiation and development as well as proliferation and of immune, lymphatic and hematologic cells were exerted at high rates, whereas apoptotic pathways were active in dental pulp from aged donors (26). CD29 is regarded to be one of the most important cell surface markers in human neuron stem cells (36).…”

Section: Discussionmentioning

confidence: 99%

Derivation and growth characteristics of dental pulp stem cells from patients of different ages

Zhou

et al. 2015

Molecular Medicine Reports

View full text Add to dashboard Cite

Abstract. The dental pulp contains a relatively low number of stem cells; however, it is considered to be a promising source of stem cells for use in regenerative therapy. To date, it has remained elusive whether there are certain differences in the dental pulp stem cells (DPSCs) from donors of different ages. In the present study, DPSC lines were derived using teeth from children, adolescents, adults and aged donors. The derivation efficiency, the proliferative and apoptotic rate, cell marker expression and the differentiation capacity were investigated and compared among these DPSC lines. The derivation efficacy was decreased with increasing donor age. Although a large part of cell surface markers was expressed in all DPSC lines, the expression of CD29 was downregulated in the DPSCs from aged teeth. In addition, the doubling time of DPSCs from aged teeth was prolonged and the number of apoptotic cells was increased with the propagation. These DPSCs were able to differentiate into a neuronal linage, which positively expressed the neuron-specific class III beta-tubulin and microtubule-associated protein 2, as well as into an osteogenic lineage, which positively expressed CD45; however, these DPSCs from aged teeth were completely or partially deprived of differentiation capacity. By contrast, DPSCs from younger teeth displayed significantly higher vitality and a higher potential for use in dental regenerative medicine.

show abstract

Microarray Evaluation of Age-related Changes in Human Dental Pulp

Cited by 39 publications

References 29 publications

Analysis of Senescence-Related Differentiation Potentials and Gene Expression Profiles in Human Dental Pulp Stem Cells

Analysis of Senescence-Related Differentiation Potentials and Gene Expression Profiles in Human Dental Pulp Stem Cells

Oxygen Saturation in the Dental Pulp of Maxillary Premolars in Different Age Groups - Part 1

Derivation and growth characteristics of dental pulp stem cells from patients of different ages

Contact Info

Product

Resources

About