Microarray expression profile of long non‐coding <scp>RNAs</scp> in human lung adenocarcinoma

Yang, Zhiyi; Liu, Hongli; Wang, Zhaoyan; Yang, Yuling; Niu, Jie; Liu, Yuanyuan; Sun, Zhi-Liang; Chen, Yin

doi:10.1111/1759-7714.12845

Cited by 28 publications

(19 citation statements)

References 34 publications

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“…The development of obesity as well as its metabolic complications is associated with dysregulation of numerous intrinsic mechanisms, which control most key metabolic processes, including cellular growth, glucose and lipid metabolism as well as insulin sensitivity and immune response (Ruderman et al 2013;Lee and Ozcan 2014;Leite et al 2014;Tam et al 2017;Wu et al 2017). It is possible that changes in classical HLA-class II molecules and non-classical HLA-class I molecules, which we observed in childhood obesity with and without insulin resistance, can contribute to these numerous intrinsic mechanisms of glucose and lipid metabolism dysregulation as well as cellular growth, because HLA-DR receptor and non-classical HLA-class I molecules play an important role in the regulation of both immune response and proliferative processes and participate in endoplasmic reticulum stress, an important factor of insulin resistance and obesity (Schaiff et al 1992;Minchenko et al 2005;Yuzefovych et al 2013;Han and Kaufman 2014;Lee and Ozcan 2014;Carosella et al 2015;Walentowicz-Sadlecka et al 2018;Yang et al 2018). Furthermore, there is data that both HLA-DR receptor and non-classical HLA-class I molecules play an important role in tumorigenesis (Kochan et al 2013;Morandi et al 2013;Leite et al 2014;Zeestraten et al 2014;Harada et al 2015;Ishigami et al 2015;Brown et al 2016;Martinez-Canales et al 2017;Winkler et al 2017;Walentowicz-Sadlecka et al 2018;Wu et al 2018).…”

Section: Discussionmentioning

confidence: 79%

Insulin resistance in obese adolescents affects the expression of genes associated with immune response

Minchenko

2019

Endocrine Regulations

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Objective. The development of obesity and its metabolic complications is associated with dysregulation of various intrinsic mechanisms, which control basic metabolic processes through changes in the expression of numerous regulatory genes.Methods. The expression level of HLA-DRA, HLA-DRB1, HLA-G, HLA-F, and NFX1 genes as well as miR-190b was measured in the blood of obese adolescents without signs of resistance to insulin and with insulin resistance in comparison with the group of relative healthy control individuals without signs of obesity.Results. It was shown that obesity without signs of insulin resistance is associated with upregulation of the expression level of HLA-DRA and HLA-DRB1 genes, but with down-regulation of HLA-G gene expression in the blood as compared to control group of relative healthy adolescents. At the same time, no significant changes were observed in the expression level of HLA-F and NFX1 genes in the blood of this group of obese adolescents. Development of insulin resistance in obese individuals leads to significant down-regulation of HLA-DRA, HLA-DRB1, HLA-G, and HLA-F gene expressions as well as to up-regulation of NFX1 gene as well as microRNA miR-190b in the blood as compared to obese patients without signs of insulin resistance.Conclusions. Results of this study provide evidence that obesity affects the expression of the subset of genes related to immune response in the blood and that development of insulin resistance in obese adolescents is associated with strong down-regulation of the expressions of HLA-DRA, HLA-DRB1, HLA-F, and HLA-G genes, which may be contribute to the development of obesity complications. It is possible that transcription factor NFX1 and miR-190b participate in downregulation of HLA-DRA gene expression in the blood of obese adolescents with insulin resistance.

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Section: Discussionmentioning

confidence: 79%

Insulin resistance in obese adolescents affects the expression of genes associated with immune response

Minchenko

2019

Endocrine Regulations

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“…Previous microarray results showed that some LncRNAs had a higher expression in LUAD tissues with lymph node metastasis. The array data have been published 16 and the gene expression omnibus accession number is GSE115734 . The microarray results showed higher expression of Linc00426 in LUAD tissues with lymphatic metastasis (Fig.…”

Section: Resultsmentioning

confidence: 99%

Linc00426 accelerates lung adenocarcinoma progression by regulating miR-455-5p as a molecular sponge

Liu

Zhang

et al. 2020

Cell Death Dis

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Increasing lines of evidence indicate the role of long non-coding RNAs (LncRNAs) in gene regulation and tumor development. Hence, it is important to elucidate the mechanisms of LncRNAs underlying the proliferation, metastasis, and invasion of lung adenocarcinoma (LUAD). We employed microarrays to screen LncRNAs in LUAD tissues with and without lymph node metastasis and revealed their effects on LUAD. Among them, Linc00426 was selected for further exploration in its expression, the biological significance, and the underlying molecular mechanisms. Linc00426 exhibits ectopic expression in LUAD tissues and cells. The ectopic expression has been clinically linked to tumor size, lymphatic metastasis, and tumor differentiation of patients with LUAD. The deregulation of Linc00426 contributes to a notable impairment in proliferation, invasion, metastasis, and epithelial–mesenchymal transition (EMT) in vitro and in vivo. Mechanistically, the deregulation of Linc00426 could reduce cytoskeleton rearrangement and matrix metalloproteinase expression. Meanwhile, decreasing the level of Linc00426 or increasing miR-455-5p could down-regulate the level of UBE2V1. Thus, Linc00426 may act as a competing endogenous RNA (ceRNA) to abate miR-455-5p-dependent UBE2V1 reduction. We conclude that Linc00426 accelerates LUAD progression by acting as a molecular sponge to regulate miR-455-5p, and may be a potential novel tumor marker for LUAD.

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“…Only one lncRNA HLA-F-AS1 in the core ceRNA subnetwork was validated in the external datasets. Several studies had revealed the dysregulation of HLA-F-AS1 in cancers [ 48 , 49 , 50 ] while the transcript expression of HLA-F-AS1 was verified to be downregulated in lung adenocarcinoma (LAD) samples [ 49 ]. It was over-expressed in colorectal cancer (CRC) tissues and CRC cell lines.…”

Section: Discussionmentioning

confidence: 99%

Identifying an lncRNA-Related ceRNA Network to Reveal Novel Targets for a Cutaneous Squamous Cell Carcinoma

Dong

Deng

et al. 2021

Biology

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A cutaneous squamous cell carcinoma (cSCC) derived from keratinocytes is the second most common cause of non-melanoma skin cancer. The accumulation of the mutational burden of genes and cellular DNA damage caused by the risk factors (e.g., exposure to ultraviolet radiation) contribute to the aberrant proliferation of keratinocytes and the formation of a cSCC. A cSCC encompasses a spectrum of diseases that range from recursor actinic keratosis (AK) and squamous cell carcinoma (SCC) in situ (SCCIS) to invasive cSCCs and further metastatic SCCs. Emerging evidence has revealed that lncRNAs are involved in the biological process of a cSCC. According to the ceRNA regulatory theory, lncRNAs act as natural miRNA sponges and interact with miRNA response elements, thereby regulating the mRNA expression of their down-stream targets. This study was designed to search for the potential lncRNAs that may become potential therapeutic targets or biomarkers of a cSCC. Considering the spirit of the study to be adequately justified, we collected microarray-based datasets of 19 cSCC tissues and 12 normal skin samples from the GEO database (GSE42677 and GSE45164). After screening the differentially expressed genes via a limma package, we identified 24 differentially expressed lncRNAs (DElncRNAs) and 3221 differentially expressed mRNAs (DEmRNAs). The miRcode, miRTarBase, miRDB and TargetScan databases were used to predict miRNAs that could interact with DElncRNAs and DEmRNAs. A total of 137 miRNA-lncRNA and 221 miRNA-mRNA pairs were retained in the ceRNA network, consisting of 31 miRNAs, 11 DElncRNAs and 155 DEmRNAs. For the functional analysis, the top enriched biological process was enhancer sequence-specific DNA binding in Gene Ontology (GO) terms. The FoxO signaling pathway, autophagy and cellular senescence were the top enrichment terms based on a Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The combination of a STRING tool and Cytoscape software (plug-in MCODE) identified five core mRNAs and built a core mRNA-associated ceRNA network. The expression for five identified core mRNAs and their related nine lncRNAs was validated using the external dataset GSE7553. Finally, one lncRNA HLA-F-AS1 and three mRNAs named AGO4, E2F1 and CCND1 were validated with the same expression patterns. We speculate that lncRNA HLA-F-AS1 may sponge miR-17-5p or miR-20b-5p to regulate the expression of CCND1 and E2F1 in the cSCC. The present study may provide potential diagnostic and therapeutic targets for cSCC patients.

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Microarray expression profile of long non‐coding RNAs in human lung adenocarcinoma

Cited by 28 publications

References 34 publications

Insulin resistance in obese adolescents affects the expression of genes associated with immune response

Insulin resistance in obese adolescents affects the expression of genes associated with immune response

Linc00426 accelerates lung adenocarcinoma progression by regulating miR-455-5p as a molecular sponge

Identifying an lncRNA-Related ceRNA Network to Reveal Novel Targets for a Cutaneous Squamous Cell Carcinoma

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