Microarray profiling and co-expression network analysis of lncRNAs and mRNAs in ovarian cancer

Abstract

Dysregulated long noncoding RNAs (lncRNAs) are involved in the pathogenesis and development of human diseases, such as epithelial ovarian cancer (EOC). In this study, we identified EOC-related lncRNAs and performed lncRNA and mRNA microarray analyses using IOSE80, a normal ovary cell line, and two ovarian carcinoma cell lines (SKOV3 and SKOV3/DDP) to investigate the potential roles of lncRNAs in EOC. lncRNA-HEIH expression in EOC tissues and cell lines was measured by quantitative real-time polymerase chain reaction (qPCR). In addition, we generated a lncRNA–mRNA co-expression network in order to identify lncRNA-expression trends and potential lncRNA target genes. Cell viability, migration, and invasion were determined by Cell Counting Kit-8, transwell assay, and wound-healing assay, respectively, and apoptosis was analyzed by flow cytometry. We identified 3527 differentially expressed lncRNAs upon comparison of the lncRNA profiles from IOSE80 with those of SKOV3 cell lines, with 11 differentially expressed lncRNAs confirmed by qPCR. Both pathway and gene ontology analyses demonstrated the involvement of lncRNAs, especially HEIH and LINC-PINT , in multiple biological processes. Furthermore, in vitro knockdown experiments confirmed that suppression of HEIH expression inhibited EOC cell proliferation. Our findings provide a foundation for further research into the role of these lncRNAs in EOC carcinogenesis and progression.

Downstream Effectors of ILK in Cisplatin-Resistant Ovarian Cancer

“…Interestingly, in osteosarcoma and gastric cancer, LINC-PINT inhibits cell invasion, migration, and proliferation by downregulating miR-21 [64,65]. In ovarian cancer, LINC-PINT is downregulated relative to normal ovary cells and tissue samples [66], whereas miR-21 is upregulated [67]. These observations warrant further investigation to elucidate the role of these lncRNAs in cisplatin-resistant ovarian cancer.…”

Novel role of lncRNA CHRF in cisplatin resistance of ovarian cancer is mediated by miR-10b induced EMT and STAT3 signaling

“…Additionally, regulations by miRNAs 5 , 6 as well as lncRNAs 7 are also epigenetic in nature. The reports on miRNAs-mediated tumorigenicity are comparatively more but, of late, there has been increasing interest in the lncRNAs in cancers in general 8 – 10 and in OC in particular 11 . Interestingly, the biological action of lncRNAs itself often involves regulation of miRNAs, including in OC 12 .…”

Long non‑coding RNA NR_002794 is upregulated in pre‑eclampsia and regulates the proliferation, apoptosis and invasion of trophoblast cells

“…Total RNA was extracted using TRIzol ® reagent (Thermo Fisher Scientific, Inc.), according to the manufacturer's instructions. To detect the expression profiles of lncRNAs and mRNAs in the placental tissues of patients with pre-eclampsia and in patients with normal pregnancies, microarray analysis was performed on lncRNAs and mRNAs in 3 pre-eclamptic and 3 matched control subjects (Affymetrix; Thermo Fisher Scientific, Inc.) (19). The placental tissues of the patients (median age, 29; range, 25–36) with pre-eclampsia and those with normal pregnancies were collected after parturition at the People's Hospital of Guangxi Zhuang Autonomous Region between April 2016 and September 2017.…”