Microarrayed allergens for IgE profiling

Deinhofer, Karin; Sevcik, Harald; Balic, Nadja; Harwanegg, Christian; Hiller, Reinhard; Rumpold, Helmut; Mueller, Manfred W.; Spitzauer, Susanne

doi:10.1016/j.ymeth.2003.08.018

Cited by 105 publications

(80 citation statements)

References 13 publications

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“…3, IgE in serum was specifically bound to the respective allergen. A comparison of the results from the allergen chip with those from a CAP assay revealed a high correlation for two allergens (R 2 = 0.91 for two mites; 0.85 for D. farinae; 0.93 for D. pteronyssinus), which is comparable to a previous report, 2,6,8 as can be seen in Fig. 4A.…”

Section: Chip-based Analysis Of Allergen-specific Ige In Serasupporting

confidence: 87%

“…These values are relatively lower than the 548 ANALYTICAL SCIENCES MAY 2007, VOL. 23 previously reported ones (around 30%), 2,8 indicating an improved reproducibility. Based on the results, it is likely that under the optimized conditions, the allergen chip provides general advantages of a chip-based assay, such as tiny amounts of sample and reagents, cost-effectiveness, and multiplexed analysis, opening up the potential of allergy diagnosis.…”

Section: Chip-based Analysis Of Allergen-specific Ige In Serasupporting

confidence: 54%

“…Released mediators resulted in various clinical symptoms, like allergic rhinitis, allergic asthma, anaphylaxis, urticaria, and angioedema. 2,3 Chronic manifestation by repeated exposure to an allergen causes an elevation of the IgE concentration in serum. For the efficient treatment and ease of allergy, a precise diagnosis of allergen-specific IgE is a prerequisite, and various assay methods have been reported, including a skin-test, 1 radio allegro sorbent assay, and a chromogenic or fluorescence enzyme immunoassay.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Critical Factors Affecting the Performance of an Allergen Chip for the Analysis of an Allergen-specific Human IgE in Serum

et al. 2007

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A sensitive and multiplexed assay of allergen-specific human immunoglobulin E (IgE) is of great significance in the precise diagnosis of allergies. We report on the optimization of critical factors for chip-based analysis of IgE in human serum with a high reliability. Extracts of two mite species were used as model allergens, and were spotted onto a glass slide for the construction of an allergen chip. Respective allergen-specific IgE in human serum was analyzed by using biotinylated anti-human IgE and a streptavidin-Cy3 conjugate. Factors affecting the performance of the allergen chip were investigated and optimized. Especially, the effect of additives, the concentrations of biotinylated anti-human IgE and the streptavidin-Cy3 conjugate, the serum dilution factor, and the concentration of allergen extract as a capturing agent were examined in detail. Under the optimized conditions, a chip-based analysis for sera from 43 patients revealed a reliable and reproducible diagnosis of respective allergies, showing a good correlation with a conventional CAP assay.

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Section: Chip-based Analysis Of Allergen-specific Ige In Serasupporting

confidence: 87%

Section: Chip-based Analysis Of Allergen-specific Ige In Serasupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Optimization of Critical Factors Affecting the Performance of an Allergen Chip for the Analysis of an Allergen-specific Human IgE in Serum

et al. 2007

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“…New options are provided by allergen microarray technology, which makes it possible to determine not only the specific antigenic protein, but also to analyse different epitopes. Such a technique has been used for inhalant and food allergens (1270)(1271)(1272)(1273)(1274)(1275)(1276). Although this method is still a research tool, it has a great potential for the future component-resolved diagnosis of allergy.…”

Section: In Vitro Testsmentioning

confidence: 99%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

3,867

1,761

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“…[132], [138] and [139] A major advantage lies in the potential to investigate large numbers of analytes in parallel while only using minute amounts of sera and antigens. Therefore, component-resolved analysis with this technology can facilitate simultaneous detection of sIgE abundance, functionality, and interaction concerning numerous allergenic determinants.…”

Section: Future Perspectivesmentioning

confidence: 99%

Potential, pitfalls, and prospects of food allergy diagnostics with recombinant allergens or synthetic sequential epitopes

Steckelbroeck¹,

Ballmer‐Weber²,

Vieths³

2008

Journal of Allergy and Clinical Immunology

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This article aims to critically review developments in food allergy diagnostics with regard to the verification of specific IgE antibodies and the identification of the responsible allergens. Results of IgE-binding tests with food extracts are hampered by cross-reactive proteins, low-quality test agents, or both. Specificity can be increased by defining adequate cutoff values, whereas sensitivity can be improved by using high-quality test agents. IgE-binding tests with purified allergens enabled reliable quantification of allergen-specific IgE titers, with higher levels found in individuals with food allergy compared with individuals without food allergy. However, the overlap in individual test reactivity between allergic and nonallergic subjects complicates interpretation. Recombinant allergens and synthetic sequential epitopes enabled detection of sensitization profiles, with IgE specific to several allergens and substructures now being suggested as markers of severity, persistence, or both. However, high-power quantitative studies with larger numbers of patients are required to confirm these markers. This article aims to critically review developments in food allergy diagnostics with regard to the verification of specific IgE antibodies and the identification of the responsible allergens. Results of IgE-binding tests with food extracts are hampered by cross-reactive proteins, low-quality test agents, or both. Specificity can be increased by defining adequate cutoff values, whereas sensitivity can be improved by using high-quality test agents. IgE-binding tests with purified allergens enabled reliable quantification of allergen-specific IgE titers, with higher levels found in individuals with food allergy compared with individuals without food allergy. However, the overlap in individual test reactivity between allergic and nonallergic subjects complicates interpretation. Recombinant allergens and synthetic sequential epitopes enabled detection of sensitization profiles, with IgE specific to several allergens and substructures now being suggested as markers of severity, persistence, or both.However, high-power quantitative studies with larger numbers of patients are required to confirm these markers. IgE-mediated and non-IgE-mediated syndromes, where IgE-mediated manifestations are responsible for the majority of food-induced, immediate-type, immune-mediated hypersensitivity reactions. 3 The development of an IgE-mediated response to food requires a series of molecular and cellular interactions, which involve antigenpresenting cells, T lymphocytes, and B lymphocytes. [4] and [5] Depending on the route of sensitization, food allergy is the result of either genuine reactivity to comestibles through the gastrointestinal tract (class I food allergens) or secondary sensitization to cross-reactive food allergens as a consequence of the initial reactivity to homologous pollen-related allergens (class II food allergens). [6] and [7] The majority of class I food allergens are heat stable and resistan...

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Microarrayed allergens for IgE profiling

Cited by 105 publications

References 13 publications

Optimization of Critical Factors Affecting the Performance of an Allergen Chip for the Analysis of an Allergen-specific Human IgE in Serum

Optimization of Critical Factors Affecting the Performance of an Allergen Chip for the Analysis of an Allergen-specific Human IgE in Serum

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Potential, pitfalls, and prospects of food allergy diagnostics with recombinant allergens or synthetic sequential epitopes

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