Microarrays towards nanoarrays and the future Next Generation of Sequencing methodologies (NGS)

Bracamonte, A. Guillermo

doi:10.1016/j.sbsr.2022.100503

Cited by 4 publications

(2 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microarrays were used to evaluate genetic expression, in the earlier moments of sequencing development, and it was a cheaper option, requiring less computational power [90] Even though this technique is still in use [91,92], the need of having previous molecular knowledge on the targeted species, limited the species compatible with this technique [93,94], and the NGS development led to the reduced interest of microarrays. Nevertheless, they can still be relevant to evaluate gene expression in model species [95][96][97].…”

Section: Evolution Of Transcriptomics Technologiesmentioning

confidence: 99%

Decoding the Transcriptome of Sharks, Rays, and Chimaeras: Insights into Their Physiology, Morphology, Evolution, and Biomedical Applications

Seixas

Domingues

Antunes

2023

Fishes

View full text Add to dashboard Cite

Chondrichthyes (including sharks, rays, and chimaeras) are a class of jawed cartilaginous fishes (with skeletons composed primarily of cartilage), with major relevance to the marine ecosystems and to humanity. However, cartilaginous fishes are facing various threatens, inflicting abrupt declines in their populations. Thus, critical assessment of available molecular genetic variation, particularly retrieved from Chondrichthyans' transcriptomic analyses, represents a major resource to foster genomics research in this ancient group of vertebrate species. Briefly, RNA-Seq involves the sequencing of RNA strands present on a target tissue, which can assist genome annotation and elucidate genetic features on species without a sequenced genome. The resulting information can unravel responses of an individual to environmental changes, evolutionary processes, and support the development of biomarkers. We scrutinized more than 800 RNA-Seq entries publicly available, and reviewed more than one decade of available transcriptomic knowledge in chondrichthyans. We conclude that chondrichthyans’ transcriptomics is a subject in early development, since not all the potential of this technology has been fully explored, namely their use to prospectively preserve these endangered species. Yet, the transcriptomic database provided findings on the vertebrates’ evolution, chondrichthyans’ physiology, morphology, and their biomedical potential, a trend likely to expand further in the future.

show abstract

Section: Evolution Of Transcriptomics Technologiesmentioning

confidence: 99%

Decoding the Transcriptome of Sharks, Rays, and Chimaeras: Insights into Their Physiology, Morphology, Evolution, and Biomedical Applications

Seixas

Domingues

Antunes

2023

Fishes

View full text Add to dashboard Cite

show abstract

“…In summary, at present, the preparation of silicon-based DNA probes is mostly at a two-dimensional level [ 17 , 18 ]. However, this kind of surface has the disadvantage of not being able to fix biomolecules at high density, and still faces great challenges in terms of sensitivity and repeatability, which also restricts the wide application of these DNA chips [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Connection of ssDNA to Silicon Substrate Based on a Mechano–Chemical Method

et al. 2023

View full text Add to dashboard Cite

A novel fabrication process to connect single-stranded DNA (ssDNA)to a silicon substrate based on a mechano–chemical method is proposed. In this method, the single crystal silicon substrate was mechanically scribed in a diazonium solution of benzoic acid using a diamond tip which formed silicon free radicals. These combined covalently with organic molecules of diazonium benzoic acid contained in the solution to form self-assembled films (SAMs). The SAMs were characterized and analyzed by AFM, X-ray photoelectron spectroscopy and infrared spectroscopy. The results showed that the self-assembled films were covalently connected to the silicon substrate by Si–C. In this way, a nano-level benzoic acid coupling layer was self-assembled on the scribed area of the silicon substrate. The ssDNA was further covalently connected to the silicon surface by the coupling layer. Fluorescence microscopy showed that ssDNA had been connected, and the influence of ssDNA concentration on the fixation effect was studied. The fluorescence brightness gradually increased with the gradual increase in ssDNA concentration from 5μmol/L to 15μmol/L, indicating that the fixed amount of ssDNA increased. However, when the concentration of ssDNA increased from 15μmol/L to 20μmol/L, the detected fluorescence brightness decreased, indicating that the hybridization amount decreased. The reason may be related to the spatial arrangement of DNA and the electrostatic repulsion between DNA molecules. It was also found that ssDNA junctions on the silicon surface were not very uniform, which was related to many factors, such as the inhomogeneity of the self-assembled coupling layer, the multi-step experimental operation and the pH value of the fixation solution.

show abstract

Bi-coloured enhanced luminescence imaging by targeted switch on/off laser MEF coupling for synthetic biosensing of nanostructured human serum albumin

Palacios,

Martinez,

Tettamanti

et al. 2023

Photochem Photobiol Sci

View full text Add to dashboard Cite

Microarrays towards nanoarrays and the future Next Generation of Sequencing methodologies (NGS)

Cited by 4 publications

References 61 publications

Decoding the Transcriptome of Sharks, Rays, and Chimaeras: Insights into Their Physiology, Morphology, Evolution, and Biomedical Applications

Decoding the Transcriptome of Sharks, Rays, and Chimaeras: Insights into Their Physiology, Morphology, Evolution, and Biomedical Applications

Connection of ssDNA to Silicon Substrate Based on a Mechano–Chemical Method

Bi-coloured enhanced luminescence imaging by targeted switch on/off laser MEF coupling for synthetic biosensing of nanostructured human serum albumin

Contact Info

Product

Resources

About