“…OT are a versatile manipulation technique, as they can be used to trap and assemble a variety of objects with different shapes, sizes, and material compositions, including dielectric microspheres [99,101,102,106,125,127,128], metallic nanoparticles [126,129] nanowires, [110,[130][131][132][133][134][135], and biological media [104,124,[136][137][138][139]. While OT can effectively position a diverse array of objects in 3D, it is necessary to immobilize these objects in the absence of the optical trap to form self-standing structures.…”