Microbe-loaded bioink designed to support therapeutic yeast growth

Etter, Emma L.; Heavey, Mairead K.; Errington, Matthew; Nguyen, Juliane

doi:10.1039/d3bm00514c

Cited by 3 publications

(1 citation statement)

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“…There has been a steady rise in the development and use of therapeutic patches 3 , 64 – 67 over the last few decades for a wide range of applications including myocardial infarction 68 , chronic wounds 69 , organ hemorrhage 8 , 9 , and cancer 70 , 71 . However, most of these patches do not readily adhere to wet and dry tissues and do not consider the complex mechanics and volumetric changes of dynamic organs caused by their auxetic and anisotropic properties.…”

Section: Discussionmentioning

confidence: 99%

Instantly adhesive and ultra-elastic patches for dynamic organ and wound repair

Chansoria,

Chaudhari,

Etter

et al. 2024

Nat Commun

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Bioadhesive materials and patches are promising alternatives to surgical sutures and staples. However, many existing bioadhesives do not meet the functional requirements of current surgical procedures and interventions. Here, we present a translational patch material that exhibits instant adhesion to tissues (2.5-fold stronger than Tisseel, an FDA-approved fibrin glue), ultra-stretchability (stretching to >300% its original length without losing elasticity), compatibility with rapid photo-projection (<2 min fabrication time/patch), and ability to deliver therapeutics. Using our established procedures for the in silico design and optimization of anisotropic-auxetic patches, we created next-generation patches for instant attachment to tissues while conforming to a broad range of organ mechanics ex vivo and in vivo. Patches coated with extracellular vesicles derived from mesenchymal stem cells demonstrate robust wound healing capability in vivo without inducing a foreign body response and without the need for patch removal that can cause pain and bleeding. We further demonstrate a single material-based, void-filling auxetic patch designed for the treatment of lung puncture wounds.

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Section: Discussionmentioning

confidence: 99%

Instantly adhesive and ultra-elastic patches for dynamic organ and wound repair

Chansoria,

Chaudhari,

Etter

et al. 2024

Nat Commun

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Heterogeneous and Composite Bioinks for 3D-Bioprinting of Complex Tissue

Rasouli,

Sweeney,

Frampton

2024

Biomedical Materials & Devices

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Bioink composition is a key consideration for the 3D-bioprinting of complex and stable structures used to model tissues and as tissue constructs for regenerative medicine. An emerging and industrially important area of research is the use of micro- and nanofillers to improve bioink performance without dramatically altering the physicochemical properties of the polymeric material that forms the bulk of the printed structure. The purpose of this review is to provide a comprehensive overview of emerging nanomaterial fillers designed to create heterogeneous and composite bioinks for 3D-bioprinting of complex functional tissues. We outline the criteria that must be considered when developing such a bioink and discuss applications where the fillers impart stimuli responsiveness, e.g., when exposed to magnetic fields, electrical fields, and light. We further highlight how the use of such fillers can enable non-destructive imaging to monitor scaffold placement and integrity following implantation.

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Living Biomaterials: Fabrication Strategies and Biomedical Applications

Chen,

Zhang

2024

Acc. Mater. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Natural or bioengineered living organisms (including mammalian cells, bacteria, microalgaes, yeast, viruses, plant cells, and the multiple organism community) possess many intrinsic or artificial superiorities than the synthesized and inert biomaterials for application in many fields, especially biomedical applications. By leveraging the inherent or artificial therapeutic competences (e.g., disease chemotaxis, drugs production, intelligent delivery, immune activation and metabolic regulation), these living organisms have been developed as critical therapeutic formulations for biomedical applications to solve unmet medical needs. These living organisms are more intelligent, more easily available, more highly active, and more strongly curative than conventional inert formulations, such as inorganic nanocarriers, metal−organic chelating networks, polymeric nanovesicles and biomembrane biohybrids, etc. Nevertheless, nonspecific in vivo circulation, the diseased microenvironmenttriggered inactivation, uncontrolled proliferation or colonization, unexpected side effects, and unsatisfactory therapeutic effect severely restricted their further research development and clinical approval. Living biomaterials, fabricated by integrating tailored functional materials with natural or bioengineered living organisms by chemical conjugation, physical assembly, and biological engineering strategies as well as advanced construction techniques, are rapidly developed to preserve or augment bioactivity and therapeutic properties of living organisms and even control their behaviors, decrease their biotoxicity, and impart them with new biofunctionalities, like stress resistance, bioactivity maintenance, safe trafficking, controllable proliferation and colonization, and evolved metabolism properties. These acquired capacities are especially beneficial to improve therapeutic potency and compliance, solve significant therapeutic restrictions, avoid biosafety questions, enhance therapeutic performances, and extend the boundaries of the fabricated living biomaterials on science research and practical biomedical applications. Additionally, the introduction of biocompatible and instructive functional materials, such as inorganic materials, synthetic polymers and polypeptides, functional proteins and enzymes, as well as biological component materials, can also promote the interaction of living biomaterials with the living body and provide feedback to further adapt the biofunctions of living organisms.In this Account, we present a brief overview of recent advances of living biomaterials in their fabrication strategies and biomedical applications, embracing living organism species as well as living organism communities. We introduce the typical and practicable methods and techniques for fabrication of living biomaterials, mainly including chemical conjugation, physical assembly, biological editing, and metabolic engineering. On the basis of these fabrication strategies, the representative examples ...

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Microbe-loaded bioink designed to support therapeutic yeast growth

Abstract: Live biotherapeutic products (LBPs) are an emerging class of therapeutics comprised of engineered living organisms such as bacteria or yeast. Bioprinting with living materials has now become possible using modern...

Cited by 3 publications

References 50 publications

Instantly adhesive and ultra-elastic patches for dynamic organ and wound repair

Instantly adhesive and ultra-elastic patches for dynamic organ and wound repair

Heterogeneous and Composite Bioinks for 3D-Bioprinting of Complex Tissue

Living Biomaterials: Fabrication Strategies and Biomedical Applications

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