Microbial Analysis in Primary and Persistent Endodontic Infections by Using Pyrosequencing

Hong, Bo-Young; Lee, Tae Kwon; Lim, Sang Min; Chang, Seok Woo; Park, Joonhong; Han, Seung Hyun; Zhu, Qiang; Safavi, Kamran E.; Fouad, Ashraf F.; Kum, Kee‐Yeon

doi:10.1016/j.joen.2013.05.001

Cited by 115 publications

(135 citation statements)

References 35 publications

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“…4 Understanding the microbiota composition in the infected root canal is essential for improving the treatment strategies to control infections and eliminate the pathogenic agents, as well as to prevent reinfection and periapical lesions. 9 The type and frequency of microbial agents involved in the genesis of endodontic diseases depend on several factors, such as geographic location, socioeconomic status, dietary habits, and oral hygiene status. 10 To the best of our knowledge, no study in Iran has explored this topic to date.…”

Section: Declaration Of Interestmentioning

confidence: 99%

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Pourhajibagher

Ghorbanzadeh²,

Bahador

2017

Braz. oral res.

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Endodontic infections are considered to be caused by the presence of various microorganisms within the root canal system. Recognition of this microbiota contributes to the successful treatment of infected root canals. This study investigated the microorganisms associated with primary and secondary endodontic infections via culture methods, biochemical tests, and molecular approaches in an Iranian population. Microbial specimens were collected from 36 patients with primary endodontic infection and 14 patients with a history of root canal therapy. Advanced microbiological culture techniques were used to isolate microbiota; subsequently, biochemical tests and 16S ribosomal RNA gene sequencing were performed to identify the microorganisms. Within the total 218 cultivable isolates, Veillonella parvula (20.6%) was found to occur with the highest frequency in primary endodontic infection, followed by Porphyromonas gingivalis (14.1%), and Aggregatibacter actinomycetemcomitans (9.2%). Enterococcus faecalis (36.6%) was the most predominant microorganism in secondary endodontic infections, followed by Candida albicans, Propionibacterium acnes, and V. parvula with frequencies of 20%, 2%, and 2%, respectively. It was concluded that V. parvula and E. faecalis was most frequently found in primary and secondary endodontic infections, respectively.

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Section: Declaration Of Interestmentioning

confidence: 99%

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Pourhajibagher

Ghorbanzadeh²,

Bahador

2017

Braz. oral res.

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“…The microbiological profiles of persistent endodontic infections have been analyzed by a variety of methods including conventional culture systems [4,[10][11][12], polymerase chain reaction [13], reverse-capture checkerboard analysis [14], and pyrosequencing [15]. Evidence from these studies has shown that the microbial flora from persistent endodontic infections differs markedly from that found in untreated necrotic dental pulp, quantitatively as well as qualitatively.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antifungal Effect of 405 nm Monochromatic Laser on Endodontopathogenic Microorganisms

Imamura

Tatehara

Takebe

et al. 2014

International Journal of Photoenergy

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The purpose of this study is to evaluate the usefulness of 405 nm monochromatic laser irradiation as an alternative management for prevention and/or treatment of endodontic infections. A monochromatic laser-emitting device equipped with a 405-nm laser diode was developed. Using this device, the effect of 405 nm laser irradiation on the growth of Porphyromonas gingivalis, Prevotella intermedia, Enterococcus faecalis, and Candida albicans, which are microorganisms associated with persistent endodontic infections, was evaluated by viable colony counting. As a result, the irradiation with a 405 nm laser had a significant bactericidal/fungicidal effect on P. gingivalis, P. intermedia, and C. albicans, whereas the growth of E. faecalis was not affected by the irradiation. The inhibition rate in P. gingivalis and P. intermedia was ∼60% and ∼80%, respectively, following irradiation at 0.2 W for 300 sec. The inhibition rate in C. albicans was ∼90% following irradiation at 0.2 W for 1200 sec. These results indicate that 405 nm monochromatic laser irradiation exerts a bactericidal/fungicidal effect on these microorganisms. The present study clearly demonstrates that 405 nm laser irradiation is a promising alternative management strategy for prevention and/or treatment of endodontic infections.

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“…However, this assertion was placed in doubt based on recent publications reporting that other species, such as streptococci, may be predominant pathogens associated with persistent endodontic infections. The difference may be due to different methodological approaches for sampling and detection, different clinical conditions or socio-geographical differences in the studied subjects 6,18,26 . Finally, it is necessary to advocate for a comprehensive approach to find new antibacterial substances that completely eradicate the microorganisms from the root canal system, which in its genesis is formed to maintain sterility 27 .…”

Section: Discussionmentioning

confidence: 99%

“…In cases of primary endodontic infections, there is a higher bacterial load than in secondary infections [6][7] the bacterial load is reduced by at least 95% and the remaining viable bacteria may be the cause of refractory lesions 8 . Teeth with endodontic failure have been associated with the presence of Enterococcus species [9][10] .…”

Section: Introductionmentioning

confidence: 99%

Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population

Sánchez‐Sanhueza¹,

González-Rocha²,

Dominguez³

et al. 2015

Braz. J. Oral Sci.

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Aim: To isolate and identify in a Chilean population, Enterococcus spp. from root canals with persistent chronic apical periodontitis (CAP) and to investigate the potential correlation between the bacteria and the observed clinical features. Methods: Twenty patients with indication for endodontic retreatment due to persistent CAP were selected. Data from patient general health and dental clinical history were recorded. During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. The relationship between the number of colony-forming units of Enterococcus spp. and patient clinical data was assessed statistically by the Pearson Chi square and Fisher exact tests. Finally, a Polymerase Chain Reaction (PCR) assay to determine the most prevalent species of Enterococcus spp. was conducted in the clinical samples, and the results were analyzed by a proportion comparison test. Results: Enterococcus spp. strains were isolated in 70% of the patients. Most of them (98.8%) accounted for Enterococcus faecalis and only 1.2% for Enterococcus faecium. A high frequency of E. faecalis was found in teeth with inadequate endodontic treatment or dental crown restorations. Conclusions: This study concluded that E. faecalis is prevalent in root canals with persistent CAP in a Chilean population. E. faecium as found in a single case with the poorest root canal filling. Further studies are still required to investigate the presence of other species, which may be linked to persistent chronic apical periodontitis.

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Microbial Analysis in Primary and Persistent Endodontic Infections by Using Pyrosequencing

Cited by 115 publications

References 35 publications

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Antibacterial and Antifungal Effect of 405 nm Monochromatic Laser on Endodontopathogenic Microorganisms

Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population

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