Microbial Analysis of Canals of Root-filled Teeth with Periapical Lesions Using Polymerase Chain Reaction

Gomes, Brenda Paula Figueiredo de Almeida; Pinheiro, Éricka Tavares; Jacinto, Rogério de Castilho; Zaia, Alexandre Augusto; Ferraz, Caio Cézar Randi; Souza-Filho, Francisco José de

doi:10.1016/j.joen.2008.01.016

Cited by 196 publications

(193 citation statements)

References 27 publications

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“…Various bacteria have been used in microleakage studies [17][18]21,25 . However, there is a focus on E. faecalis because of its resistance [19][20]22,24 , survival in nutrient-poor areas over long periods 26 , and association with persistent chronic periapical lesions 27 . The results demonstrated that the mean time of leakage in samples in the experimental period was 21 days.…”

Section: Discussionmentioning

confidence: 99%

Comparison of three obturation techniques with regard to bacterial leakage

Nabeshima¹,

Martins²,

Leonardo³

et al. 2013

Braz. J. Oral Sci.

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Aim: To compare bacterial leakage in root canals obturated with the modified single-cone, lateral condensation, and continuous wave of condensation techniques. Methods: Distobuccal root canals of maxillary molars were shaped up to ProTaper F2 and obturated with modified singlecone, lateral condensation or continuous wave of condensation technique. Two-chamber bacterial model using Enterococcus faecalis was employed for bacterial leakage evaluation for 30 days. The chi-square test was applied to evaluate differences between turbid and non-turbid samples, and the Kruskal-Wallis test was used for evaluating the time necessary for microleakage. A significance level of 5% was set for all analyses. Results: The modified single-cone technique showed leakage in 73.3% of samples, lateral condensation in 66.6%, and continuous wave of condensation in 53.3%, but there were no significant differences among the groups (p>0.05). Conclusions: It can be concluded that the modified single-cone technique shows similar sealing efficacy to that of lateral condensation and continuous wave of condensation technique.

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Section: Discussionmentioning

confidence: 99%

Comparison of three obturation techniques with regard to bacterial leakage

Nabeshima¹,

Martins²,

Leonardo³

et al. 2013

Braz. J. Oral Sci.

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“…In primary and secondary infections, E. faecalis was found, respectively, in 54.83 and 71.42%. According to the literature, E. faecalis is often found in root canals with chronic apical periodontitis after endodontic treatment [11,32,34,35]. In systematic review, Zhang et al [11] reported that the rate of E. faecalis by both culture and PCR methods was higher in persistent infections compared with untreated chronic periapical periodontitis.…”

Section: Discussionmentioning

confidence: 99%

“…The procedures for sample collection were based on the study of Gomes et al [32]. Before the coronal opening for endodontic treatment rinsing with chlorhexidine gluconate solution (0.12%) was performed for 1 min.…”

Section: Microbiological Collectionmentioning

confidence: 99%

Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy

et al. 2017

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Introduction Enterococcus faecium has become an important microorganism in nosocomial infections with great ability to acquire antibiotic resistance. However, little is known about their presence on the oral cavity. Therefore, our objective was to verify the presence of E. faecium and E. faecalis in endodontic infections and compare their susceptibility to conventional antibiotics and photodynamic therapy. Methods We performed 38 collections from the root canals of different patients. Positive Enterococcus agar samples were submitted to phenotypic and genotypic testing for speciesspecific confirmation. The isolates identified as E. faecium and E. faecalis were tested for susceptibility to antibiotics by the E-test method. After that, the isolates were evaluated for susceptibility to photodynamic therapy (PDT) using methylene blue and gallium arsenide aluminum laser with a wavelength of 660 nm and fluence of 39.5 J/cm 2 (energy of 15 J and time of 428 s).Results Cultures positive for E. faecalis were found in 22 patients (58%). Among these patients, only two had E. faecium in mixed infections with E. faecalis. In the isolates of E. faecalis, 27% were resistant to antibiotics, including tetracycline, ciprofloxacin, and azithromycin. The isolates of E. faecium showed no resistance to the antibiotics tested. Both the isolates of E. faecium and E. faecalis exhibit significant susceptibility to PDT, including the isolates resistant to antibiotics. The reductions achieved by PDT ranged of 2.76 to 4.31 log 10 for E. faecalis strains and of 3.93 to 4.33 log 10 for E. faecium strains. Conclusion E. faecium showed lower prevalence in endodontic infections and higher susceptibility to antibiotics when compared to E. faecalis. In in vitro assays, PDT had a significant antimicrobial activity for both strains.

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“…7 E. faecalis, endodontik tedaviden sonra başarısızlığa uğramış vakalardan en çok izole edilen mikroorganizmalardandır. [8][9][10][11][12] Enterokokların yanı sıra streptokoklar da birçok antimikrobiyal ajana yüksek direnç geliştirmiş ve hastane enfeksiyonlarında sıkça rastlanılan bakterilerdir. Yerleştirilen MTA diskinin agar jel ile temas etmediği noktalar olursa, bu noktalardan yanlış negatif cevap alınabilir.…”

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