Microbial analysis of endodontic infections in teeth with post‐treatment apical periodontitis before and after medication

Abstract

This study aimed to determine the intraradicular microbiota of previously root canal‐treated teeth with apical periodontitis and to investigate the antibacterial effectiveness of different intracanal medicaments. Sixteen patients with post‐treatment apical periodontitis were allocated into two groups according to the intracanal medicament used: calcium hydroxide (CH) and 2% chlorhexidine gluconate gel (CHX) group. Total bacterial loads, as well as the amount of Enterococcus faecalis (E. faecalis) were determined before (S1) and after (S2) chemomechanical preparation and finally, after intracanal medication (S3) by means of ddPCR. The unpaired t test was used to compare parametric. S3‐total bacteria copy number of the CH group was lower than the CHX group (p < 0.05). There was no statistical difference between the CHX‐ and the CH groups in terms of E.faecalis copy number (p > 0.05). But in terms of total bacteria, CH is better than CHX. Consequently, CH can be used to optimise the antibacterial efficiency of chemomechanical preparation in previously root canal‐treated teeth with apical periodontitis.

Comparation of the effectiveness of conventional needle irrigation and photon-induced photoacoustic streaming with sodium hypochorite in the treatment of teeth with apical periodontitis: a randomized clinical trial

“…The disparities observed between these two studies could potentially be attributed to variations in case selection and the methodology employed for microbiological analysis. Studies have highlighted the limitations of microbial culture assays in accurately quantifying culturable bacteria during biofilm assessment [ 29 , 30 ]. In contrast, ATP biotechnologies, which rely on nucleic acid measurements, have demonstrated higher sensitivity compared to bacterial culture methods [ 31 ].…”