Microbial and Functional Profile of the Ceca from Laying Hens Affected by Feeding Prebiotics, Probiotics, and Synbiotics

Pineda-Quiroga, C.; Borda-Molina, Daniel; Chaves‐Moreno, Diego; Ruíz, R.; Atxaerandio, Raquel; Camarinha‐Silva, Amélia; García-Rodriguez, A.

doi:10.3390/microorganisms7050123

Cited by 26 publications

(18 citation statements)

References 38 publications

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“…Butyrate could enhance epithelial regeneration by stimulating villus growth; however, it does not inhibit C. perfringens (61,62). Absorption of butyrate and propionate by chicken cecal mucosa could improve host energy metabolism and improve performance (63). Similar to this study, supplementation of B. licheniformis enriched butanoate metabolism in the microbiota of broiler chickens challenged with NE compared to challenged non-supplemented group (64).…”

Section: Discussionsupporting

confidence: 62%

Effect of Probiotics and Multi-Component Feed Additives on Microbiota, Gut Barrier and Immune Responses in Broiler Chickens During Subclinical Necrotic Enteritis

et al. 2020

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The withdrawal of antibiotic growth promoters from poultry feed has increased the risk of necrotic enteritis (NE) outbreaks. This study examined the effects of a probiotic (PROB) or probiotic/prebiotic/essential oil supplement (PPEO) during a subclinical NE challenge. On day (d) of hatch, 960 male broilers were randomized to four groups (8 pens/treatment, 30 birds/pen) including (1) negative control (NC): corn-soybean meal diet; (2) positive control (PC): NC + 20 g Virginiamycin/ton diet; (3) NC + 227 g PROB/ton diet; and (4) NC + 453 g PPEO/ton diet. One d after placement, birds were challenged by a coccidia vaccine to induce NE. Feed intake and body weights were measured on d 8 (NE onset) and end of each feeding period. On d 8, the small intestines of three birds/pen were examined for NE lesions. Jejunum samples and ileal mucosal scrapings from one bird/pen were respectively collected to measure mRNA abundance (d 8 and d 14) and profile the microbiota (d 8 and d 42). Data were analyzed in JMP or QIIME 2 and significance between treatments identified by LSD (P < 0.05). PROB and PPEO had significantly lower mortality (d 0–14) and NE lesion scores compared to NC. Feed conversion ratio was significantly lower in PC, PROB, and PPEO, while average daily gain was higher in PPEO and PC groups compared to NC from d 0–42. On d 8 and d 14, mRNA abundance of claudin-3 was higher in PPEO compared to NC. On d 14, compared to NC, mRNA abundance of sIgA and PGC-1α in PROB and PPEO were lower and higher, respectively. Compared to NC, PPEO increased mTOR abundance on d 14. On d 8, relative abundance of Clostridium sensu stricto 1, Ruminiclostridium9, Prevotellaceae, Prevotellaceae UCG-014, ASF356, and Muribaculaceae was higher in NC compared to PPEO and PROB, while Lactobacillus was lower in NC. Escherichia-Shigella had higher abundance in PC compared to PPEO and PROB. Collectively, these data indicate that during a subclinical naturally occurring NE, supplementation of PROB or PPEO supports performance and reduces intestinal lesions, potentially through modifying tight junction proteins, gut microbiota, immune responses, and cell metabolism.

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Section: Discussionsupporting

confidence: 62%

Effect of Probiotics and Multi-Component Feed Additives on Microbiota, Gut Barrier and Immune Responses in Broiler Chickens During Subclinical Necrotic Enteritis

et al. 2020

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“… 5 Myo-inositol, starting from phytic acid (inositol hexaphosphate or IP6), can be released into the intestine of monogastric animals by the activity of enzymatic phytases, a process that occurs in the intestinal mucosa. 4 , 15 , 20 Phytases (myo-inositol hexaphosphate phosphohydrolase) are found in plants, microorganisms, including bacteria, and animal tissues. 16 , 17 , 18 It can release free inositol, orthophosphate and intermediate products including forms of inositol.…”

Section: Inositol and The Gut Microbiotamentioning

confidence: 99%

“…Interestingly, diet also has an essential influence on the establishment of the cecum microbial communities in poultry, so its supplementation with additives, such as probiotics (2 g/kg of addition of phytic acid), prebiotics, and symbiotics has been claimed to improve animal health. 20 Olsenella spp., and Lactobacillus crispatus increased their abundance in prebiotic and symbiotic treatments in this animal model. Furthermore, metagenomes revealed that the genes encoding for the metabolism of butanoate, propanoate, InsP, and galactose were more abundant during a prebiotic diet.…”

Section: Inositol and The Gut Microbiotamentioning

confidence: 99%

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Inositols and metabolic disorders: From farm to bedside

Caputo

Bona

Leone

et al. 2020

Journal of Traditional and Complementary Medicine

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Inositol and its derivates are catching interest in metabolism since taking part in several physiological processes, including endocrine modulation. Through several mechanisms mostly mediated by insulin signaling, these compounds regulate the activities of several hormones and are essential in oocytes maturation. It is interesting to point out the contribution of an inositol deficiency in the development of several diseases, mainly in the metabolic and endocrine setting. Inositols derive from both diet and endogenous production; among causes of inositol deficiency reduced dietary intake, increased catabolism and/or excretion, decreased biosynthesis, inhibition of gut and cellular uptake and altered microbiota could be considered. Mounting direct and indirect evidence suggests that the two main isoforms (Myo-inositol-inositol, D-chiro-inositol) are implied in glycemic and lipidic metabolism and supplementation yield a beneficial effect on these parameters without hazards for health. Moreover, they have a role in polycystic ovary syndrome, acting as insulin-sensitizing agents and free radical scavengers, helping to regulate metabolism and promoting ovulation. The aim of this narrative review is to discuss the role of inositols in metabolic function disorders paying attention to whether these compounds could be efficacious and safe as a therapeutic agent with a focus on dietary intake and the role of gut microbiota.

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“…Variability in the GIT microbiota is well documented and factors such as genotype, species, bedding material, diet, and sex contribute to differences in composition [15,19,48,49,50]. Even when these factors are controlled for, individual bird-to-bird variation in the GIT microbiota exists, potentially arising from the inherent nature of modern poultry production practices [9,20,51,52].…”

Section: Poultry Species Differencesmentioning

confidence: 99%

The Microbial Pecking Order: Utilization of Intestinal Microbiota for Poultry Health

et al. 2019

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The loss of antibiotics as a tool to improve feed efficiency in poultry production has increased the urgency to understand how the microbiota interacts with animals to impact productivity and health. Modulating and harnessing microbiota-host interactions is a promising way to promote poultry health and production efficiencies without antibiotics. In poultry, the microbiome is influenced by many host and external factors including host species, age, gut compartment, diet, and environmental exposure to microbes. Because so many factors contribute to the microbiota composition, specific knowledge is needed to predict how the microbiome will respond to interventions. The effects of antibiotics on microbiomes have been well documented, with different classes of antibiotics having distinctive, specific outcomes on bacterial functions and membership. Non-antibiotic interventions, such as probiotics and prebiotics, target specific bacterial taxa or function to enhance beneficial properties of microbes in the gut. Beneficial bacteria provide a benefit by displacing pathogens and/or producing metabolites (e.g., short chain fatty acids or tryptophan metabolites) that promote poultry health by improving mucosal barrier function or immune function. Microbiota modulation has been used as a tool to reduce pathogen carriage, improve growth, and modulate the immune system. An increased understanding of how the microbiota interacts with animal hosts will improve microbiome intervention strategies to mitigate production losses without the need for antibiotics.

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Microbial and Functional Profile of the Ceca from Laying Hens Affected by Feeding Prebiotics, Probiotics, and Synbiotics

Cited by 26 publications

References 38 publications

Effect of Probiotics and Multi-Component Feed Additives on Microbiota, Gut Barrier and Immune Responses in Broiler Chickens During Subclinical Necrotic Enteritis

Effect of Probiotics and Multi-Component Feed Additives on Microbiota, Gut Barrier and Immune Responses in Broiler Chickens During Subclinical Necrotic Enteritis

Inositols and metabolic disorders: From farm to bedside

The Microbial Pecking Order: Utilization of Intestinal Microbiota for Poultry Health

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