Bacteria and fungi are the most active decomposers of organic materials in soil. They directly affect plant nutrient availability, and chemical and physical properties of soils. This investigation aimed at quantifying the effect of several organic materials on microbial activity of a Rhodic Eutrudox. Soil samples were incubated over a period of 91 days with the following organic materials: cattle manure (CM), earthworm humus (HM), and city sewage sludge from Barueri (BA) and Franca (FR). The activities of cellulase, protease and urease enzymes, the soil microbial carbon content (by fumigation-extraction method) and the exothermal effect were evaluated. Experimental design was randomized and arranged as factorial scheme five treatments × seven samplings with five replications. Organic materials promoted oscillations in microbial carbon (mg C g -1 soil), and enzyme activities -cellulase (µg glucose g -1 soil 24 h ) e no efeito térmico total (J g -1 solo), na seguinte ordem decrescente: (1) 655.90; 49.68; 24.55; 477.90; 367.70; 32.76; 10.66; 426.29; 273.40; 18.77; 19.35; 368.00; (4) 261.00; 17.05; 9.02; 202.79; (5) controle. Correlações foram obtidas entre o carbono microbiano e as enzimas celulase e protease (r = 0.54; p < 0.001), e entre o efeito exotérmico e a protease (r = 0.34; p < 0.005). A atividade de enzimas esteve diretamente relacionada à microbiota do solo, refletindo, dessa forma, no efeito térmico total. Palavras-chave: enzimas, carbono da biomassa, materiais orgânicos, microrganismos do solo, microcalorimetria