Microbial biomineralization of hydroxyapatite nanocrystals using Bacillus tequilensis

Paramasivan, Mareeswari; Kumar, Tiruchirapalli Subramaniam Sampath; Kanniyappan, Hemalatha; Muthuvijayan, Vignesh; Chandra, T. S.

doi:10.1016/j.ceramint.2022.10.138

Cited by 6 publications

(7 citation statements)

References 51 publications

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“…Both cell lines have been used extensively to investigate the interactions with nanomaterials. 26–29…”

Section: Methodsmentioning

confidence: 99%

“…Despite the importance of renal tubular cells for kidney function, their response to NPs remains understudied. 26–29…”

Section: Introductionmentioning

confidence: 99%

“…Despite the importance of renal tubular cells for kidney function, their response to NPs remains understudied. [26][27][28][29] Cultured cells are essential to assess critical aspects of nanobio interactions in a rigorous fashion. This evaluation consists of two stages.…”

Section: Introductionmentioning

confidence: 99%

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Silica-coated LiYF₄:Yb³⁺, Tm³⁺ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

Bietar,

Chu,

Mandl

et al. 2024

RSC Adv.

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“…Both cell lines have been used extensively to investigate the interactions with nanomaterials. 26–29…”

Section: Methodsmentioning

confidence: 99%

“…Despite the importance of renal tubular cells for kidney function, their response to NPs remains understudied. 26–29…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Silica-coated LiYF₄:Yb³⁺, Tm³⁺ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

Bietar,

Chu,

Mandl

et al. 2024

RSC Adv.

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“…Cytotoxicity of the prepared nanoparticles was determined by MTT assay with three different cell lines namely NIH/3T3 fibroblast cells, MG-63 osteosarcoma cells, and RAW 264.7 macrophages 15 , 19 , 20 . The cell lines were cultured to a cell density of 1 × 10 4 per well in 96 well plates and incubated with cell culture medium for 24 hours 21 .…”

Section: Methodsmentioning

confidence: 99%

“…In the present study, with a view of further enhancing the properties and symmetry of synthesized HA, ionic substitutions was investigated for the first time in Serratia sp . biomineralized HA adopting a biomimetic method of synthesis 15 . Both individual and co-substitution of cations was studied and characterised for its competence in terms of functional groups, crystallinity, morphology and biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic ion substituted and Co-substituted hydroxyapatite nanoparticle synthesis using Serratia Marcescens

Paramasivan

Kumar

Kanniyappan

et al. 2023

Sci Rep

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Biomimicry is becoming deep-rooted as part of bioceramics owing to its numerous functional advantages. Naturally occurring hydroxyapatite (HA) apart from primary nano structures are also characterised by various ionic substitutions. The ease of accommodating such key elements into the HA lattice is known to enhance bone healing properties of bioceramics. In this work, hydroxyapatite synthesized via biomimetic approach was substituted with individual as well as multiple cations for potential applications in bone repair. Ion substitutions of Sr, Mg and Zn was carried out on HA for the first time by using Serratia grown in a defined biomineralization medium. The individual ions of varying concentration substituted in Serratia HA (SHA) (Sr SHA, Mg SHA and Zn SHA) were analysed for crystallinity, functional groups, morphology and crystal size. All three showed decreased crystallinity, phase purity, large agglomerated aggregates and needle-shaped morphologies. Fourier transform infrared spectroscopy (FTIR) spectra indicated increased carbonate content of 5.8% resembling that of natural bone. Additionally, the reduced O–H intensities clearly portrayed disruption of HA lattice and subsequent ion-substitution. The novelty of this study lies primarily in investigating the co-substitution of a combination of 1% Sr, Zn and Mg in SHA and establishing the associated change in bone parameters. Scanning electron microscope (SEM) and transmission electron microscope (TEM) images clearly illustrated uniform nano-sized agglomerates of average dimensions of 20–50 nm length and 8–15 nm width for Sr SHA; 10–40 nm length and 8–10 nm width for both Zn SHA and Mg SHA and 40–70 nm length and 4–10 nm width in the case of 1% Sr, Zn, Mg SHA. In both individual as well as co-substitutions, significant peak shifts were not observed possibly due to the lower concentrations. However, cell volumes increased in both cases due to presence of Sr2+ validating its dominant integration into the SHA lattice. Rich trace ion deposition was presented by energy dispersive X-ray spectroscopy (EDS) and quantified using inductively coupled plasma optical emission spectrometer (ICP-OES). In vitro cytotoxicity studies in three cell lines viz. NIH/3T3 fibroblast cells, MG-63 osteosarcoma cells and RAW 264.7 macrophages showed more than 90% cell viability proving the biocompatible nature of 1% Sr, Zn and Mg in SHA. Microbial biomineralization by Serratia produced nanocrystals of HA that mimicked “bone-like apatite” as evidenced by pure phase, carbonated groups, reduced crystallinity, nano agglomerates, variations in cell parameters, rich ion deposition and non-toxic nature. Therefore ion-substituted and co-substituted biomineralized nano SHA appears to be a suitable candidate for applications in biomedicine addressing bone injuries and aiding regeneration as a result of its characteristics close to that of the human bone.

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Engineered functional doped hydroxyapatite coating on titanium implants for osseointegration

Li,

Zhang,

Liao

et al. 2023

Journal of Materials Research and Technology

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Microbial biomineralization of hydroxyapatite nanocrystals using Bacillus tequilensis

Cited by 6 publications

References 51 publications

Silica-coated LiYF₄:Yb³⁺, Tm³⁺ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

Silica-coated LiYF₄:Yb³⁺, Tm³⁺ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

Biomimetic ion substituted and Co-substituted hydroxyapatite nanoparticle synthesis using Serratia Marcescens

Engineered functional doped hydroxyapatite coating on titanium implants for osseointegration

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Microbial biomineralization of hydroxyapatite nanocrystals using Bacillus tequilensis

Cited by 6 publications

References 51 publications

Silica-coated LiYF4:Yb3+, Tm3+ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

Silica-coated LiYF4:Yb3+, Tm3+ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

Biomimetic ion substituted and Co-substituted hydroxyapatite nanoparticle synthesis using Serratia Marcescens

Engineered functional doped hydroxyapatite coating on titanium implants for osseointegration

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Product

Resources

About

Silica-coated LiYF₄:Yb³⁺, Tm³⁺ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells

Silica-coated LiYF₄:Yb³⁺, Tm³⁺ upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells