Microbial biopolymers in articular cartilage tissue engineering

BİNGÜL, Nur Deniz; Öz, Yunus Emre; Ürkmez, Aylin Şendemir; Hameş, Elif Esin

doi:10.1007/s10965-022-03178-0

Cited by 6 publications

(2 citation statements)

References 154 publications

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“…The regeneration of articular cartilage has been an important topic in both academic and clinical fields for a long time [54][55][56][57][58][59][60][61][62]. Although efforts had been made by plenty of researchers, very few materials could turn into effective biomedical products.…”

Section: Discussionmentioning

confidence: 99%

Wet 3D printing of biodegradable porous scaffolds to enable room-temperature deposition modeling of polymeric solutions for regeneration of articular cartilage

Yu,

Wang,

Gao

et al. 2024

Biofabrication

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Tissue engineering has emerged as an advanced strategy to regenerate various tissues using different raw materials, and thus it is desired to develop more approaches to fabricate tissue engineering scaffolds to fit specific yet very useful raw materials such as biodegradable aliphatic polyester like poly(lactide-co-glycolide) (PLGA). Herein, a technique of “wet 3D printing” was developed based on a pneumatic extrusion 3D printer after we introduced a solidification bath into a 3D printing system to fabricate porous scaffolds. The room-temperature deposition modeling of polymeric solutions enabled by our wet 3D printing method is particularly meaningful for aliphatic polyester, which otherwise degrades at high temperature in classic fuse deposition modeling. As demonstration, we fabricated a bilayered porous scaffold consisted of PLGA and its mixture with hydroxyapatite (HAp) for regeneration of articular cartilage and subchondral bone. Long-term in vitro and in vivo degradation tests of the scaffolds were carried out up to 36 weeks, which support the three-stage degradation process of the polyester porous scaffold and suggest faster degradation in vivo than in vitro. Animal experiments in a rabbit model of articular cartilage injury were conducted. The efficacy of the scaffolds in cartilage regeneration was verified through histological analysis, micro-CT and biomechanical tests, and the influence of scaffold structures (bilayer versus single layer) on in vivo tissue regeneration was examined. This study has illustrated that the wet 3D printing is an alternative approach to biofabricate tissue engineering porous scaffolds based on biodegradable polymers.

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Section: Discussionmentioning

confidence: 99%

Wet 3D printing of biodegradable porous scaffolds to enable room-temperature deposition modeling of polymeric solutions for regeneration of articular cartilage

Yu,

Wang,

Gao

et al. 2024

Biofabrication

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“…Therefore, aerogels made from natural polymers have been widely employed in bioengineering for tissue regeneration. These aerogels have been proven to offer an adequate bioactive microenvironment and strength to promote the development of new chondral muscles at damaged locations [ 171 , 172 ].…”

Section: Biomedical Applications Of Aerogelmentioning

confidence: 99%

Emerging Trends in Nanotechnology: Aerogel-Based Materials for Biomedical Applications

et al. 2023

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At present, aerogel is one of the most interesting materials globally. The network of aerogel consists of pores with nanometer widths, which leads to a variety of functional properties and broad applications. Aerogel is categorized as inorganic, organic, carbon, and biopolymers, and can be modified by the addition of advanced materials and nanofillers. Herein, this review critically discusses the basic preparation of aerogel from the sol–gel reaction with derivation and modification of a standard method to produce various aerogels for diverse functionalities. In addition, the biocompatibility of various types of aerogels were elaborated. Then, biomedical applications of aerogel were focused on this review as a drug delivery carrier, wound healing agent, antioxidant, anti-toxicity, bone regenerative, cartilage tissue activities and in dental fields. The clinical status of aerogel in the biomedical sector is shown to be similarly far from adequate. Moreover, due to their remarkable properties, aerogels are found to be preferably used as tissue scaffolds and drug delivery systems. The advanced studies in areas including self-healing, additive manufacturing (AM) technology, toxicity, and fluorescent-based aerogel are crucially important and are further addressed.

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Fabrication of porous bone scaffolds using degradable and mouldable bacterial cellulose

Öz,

Bingül,

Morçimen

et al. 2024

Cellulose

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Bacterial cellulose (BC) is a biomaterial extensively studied in tissue engineering due to its favorable properties. Porosity, biocompatibility, biodegradability and mechanical durability are essential material properties for scaffold use in tissue engineering. This study aims to fabricate porous scaffolds using a moldable and degradable BC-HAp composite for bone tissue engineering. BC was produced by Komagataeibacter sucrofermentans under static culture conditions. The harvested BC membranes were purified and then mechanically shredded. BC oxidation was performed using different sodium periodate concentrations (0.05–0.5 M) and treatment times (0.5–12 h). Oxidized BCs (oxBC) were modified with hydroxyapatite (HAp), then were moulded, lyophilized, and characterized. The degradability of the scaffolds was determined for 45 days. Cytotoxic analysis of oxBC scaffolds was carried out for 7 days using the L929 fibroblast cell line. The oxidation degrees of the shredded BC samples were between 6.75 and 81%, which increased in line with the increasing concentration and application time of periodate. The scaffolds prepared using oxidized cellulose for 30 and 60 min (oxBC30 and oxBC60) preserved their integrity, These scaffolds showed a weight loss of 9% and 14% in 45 days, respectively. The pore distribution was between 50 and 450 µm and concentrated in the 50–150 µm range. The compression moduli were 88.72 kPa and 138.88 kPa for oxBC30-HAp and oxBC60-HAp, respectively. It was determined that oxBC did not show a significant difference in cell viability compared to the control groups and was not cytotoxic. In conclusion, degradable and more porous bone scaffolds were fabricated using mouldable oxBC.

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Microbial biopolymers in articular cartilage tissue engineering

Cited by 6 publications

References 154 publications

Wet 3D printing of biodegradable porous scaffolds to enable room-temperature deposition modeling of polymeric solutions for regeneration of articular cartilage

Wet 3D printing of biodegradable porous scaffolds to enable room-temperature deposition modeling of polymeric solutions for regeneration of articular cartilage

Emerging Trends in Nanotechnology: Aerogel-Based Materials for Biomedical Applications

Fabrication of porous bone scaffolds using degradable and mouldable bacterial cellulose

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