Microbial communities responsible for the decomposition of rice straw compost in a Japanese rice paddy field determined by phospholipid fatty acid (PLFA) analysis

Bacterial Communities Responsible for the Decomposition of Rice Straw Compost in a Japanese Rice Paddy Field Estimated by DGGE Analysis of Amplified 16S rDNA and 16S rRNA Fragments

“…Sugano et al (2005) studied the bacterial community structure during the decomposition of rice straw incorporated into a Japanese paddy field by 16s rDNA PCR-DGGE analysis. We have recently investigated the PLFA composition of microbiota in rice straw compost incorporated into a Japanese paddy field under flooded conditions (Tanahashi et al 2004). Our results showed that the microbial communities changed gradually along with the decomposition process of rice straw compost and were divided into two groups before and after mid-season drainage.…”

“…These changes were mainly attributed to the increase or decrease of the band intensity in bacterial DNA and the disappearance of certain bands also influenced the changes in the patterns of microbial RNA. This suggested that mid-season drainage affected the bacterial communities in rice straw compost during the decomposition of rice straw compost in paddy field soil, and that the succession of the communities was gradual, except for the period of mid-season drainage, as evidenced by the PLFA composition, probably due to the slow decomposition (about 20% for 3 months) of the compost under flooded conditions (Tanahashi et al 2004). Eight common bands (d, f, y, 2e, 2w, 2x and 3a) were observed for bacterial DNA throughout the period, while only two bands (2a and 2s) were commonly detected for bacterial RNA.…”

“…The rice straw compost samples used in the present study were the same as those used for the PLFA pattern analyses (Tanahashi et al 2004) of microbiota in rice straw compost buried into a Japanese flooded paddy field. These samples were collected from the same composting yard as that used in the studies on the microbiota in the composting process of rice straw by Cahyani et al (2002Cahyani et al ( , 2003.…”

“…The second objective of the present study was, therefore, to obtain information on potentially active members of the bacterial communities in the rice straw compost by analyzing 16s rRNA as well as 16s rDNA. We also examined the community structure by comparing the results with the previous ones (Tanahashi et al 2004) obtained by PLFA analysis as well as those of the decomposing rice straw in soil (Sugano et al 2005) and of the composting process of rice straw (Cahyani et al 2003) obtained by DGGE analysis.…”

“…In the present study, we conducted DGGE analyses targeting 16s rDNA and 16s rRNA, followed by sequencing for the rice straw compost samples buried into the Japanese paddy field used in our previous study (Tanahashi et al 2004). Thus, we described the succession and phylogenetic composition of the bacterial communities responsible for the decomposition of rice straw compost under flooded conditions during the cultivation period of paddy rice.…”