Microbial community competition rather than high-temperature predominates ARGs elimination in swine manure composting

Chen, Zhiqiang; Fu, Qiqi; Wen, Quan; Wu, Yiqi; Bao, Huanyu; Guo, Jingkun

doi:10.1016/j.jhazmat.2021.127149

Cited by 63 publications

(16 citation statements)

References 31 publications

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“…When soil pH and organic matter content were low, the expression of ARGs was inhibited. The results of this study are consistent with the conclusions of Zhang et al and Chen et al [ 55 , 56 ]. Soil acidity also affected the abundance of ARGs, which was mainly because lower pH increased the solubility of pollutants, and a higher concentration of pollutants affected the metabolism of bacteria, which has been confirmed by a previous study [ 51 ].…”

Section: Resultssupporting

confidence: 93%

Effects of Oxytetracycline/Lead Pollution Alone and in the Combined Form on Antibiotic Resistance Genes, Mobile Genetic Elements, and Microbial Communities in the Soil

Guo

Shao

et al. 2022

IJERPH

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The application of livestock manure is the leading cause of antibiotic and heavy metal pollution in agricultural soil. However, the effects of oxytetracycline (OTC) and lead (Pb) pollution in the single or combined form on antibiotic resistance genes (ARGs) in the soil need to be further studied. This study was planned to investigate the effects of OTC and Pb application on ARGs, mobile genetic elements (MGEs), and bacterial abundance in the soil. The relative abundance of ARGs and MGEs increased by 0.31-fold and 0.03-fold after the addition of 80 mg kg−1 Pb to the soil, and by 0.49-fold and 0.03-fold after the addition of 160 mg kg−1 Pb. In addition, under the premise of the existence of OTC, the inhibitory effect of a low concentration of Pb on ARG is stronger than that of a high concentration of Pb, resulting in a lower abundance of ARGs. The abundance of ARGs and MGEs increased by 0.11-fold and 0.17-fold after the addition of OTC (30 mg kg−1) to the soil at a Pb concentration of 80 mg kg−1 and by 0.18-fold and 0.04-fold at a Pb concentration of 160 mg kg−1. The addition of OTC and Pb in the soil also decreased the many bacterial communities such as Bacteroidetes, Proteobacteria, Acidobacteria, and Firmicutes. Redundancy analysis (RDA) showed that organic matter content and pH were positively correlated with the abundance of ARGs and MGEs. At the same time, electrical conductivity (EC) had a negative correlation with the abundance of ARGs and MGEs in the soil. Intl1 was significantly associated with tetB, sul1, tetQ, sul2, and sul3. Network analysis illustrated that Actinobacteria, Bacteroidetes, and Proteobacteria were the main host bacteria causing changes in the abundance of ARGs and MGEs, and they were also predominant phylum in the culture environment. This conclusion can provide a reference for the related research of ARGs in soil.

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Section: Resultssupporting

confidence: 93%

Effects of Oxytetracycline/Lead Pollution Alone and in the Combined Form on Antibiotic Resistance Genes, Mobile Genetic Elements, and Microbial Communities in the Soil

Guo

Shao

et al. 2022

IJERPH

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“…Besides, zinc elements could reduce the risk of ciprofloxacin resistance by increasing the minimum selection concentration of it (Vos et al, 2020), and could inhibit the horizontal transfer of ARGs by blocking an SOS response (Crane et al, 2018). In addition, microbial community has been proved to be one of the main reasons for ARGs propagation (Chen et al, 2022) and its shift in AD process could be significantly affected by ZnO NPs (Mu & Chen, 2011;Zhao et al, 2019). These indicate that the fate of ARGs in livestock waste AD process could be strongly influenced by feed additives like ZnO NPs, although very few studies till now have focused on this.…”

Section: Introductionmentioning

confidence: 99%

Response behavior of antibiotic resistance genes to zinc oxide nanoparticles in cattle manure thermophilic anaerobic digestion process: A metagenomic analysis

Pang

et al. 2022

Bioresource Technology

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“…In addition to differences in fecal microbial communities between livestock species, antimicrobial use policies and practices have been shown to impact the specific resistance genes that will be most prevalent in the manure [ 18 , 23 , 24 ]. Different management strategies, including composting of manure and storage time prior to application, have been shown to influence antibiotic residues and resistant bacteria within the final applied product [ 7 , 25 , 26 , 27 , 28 , 29 ]. In addition, movement of ARB and ARG through the soil can also be impacted by the nature of the manure itself; for example, the dissemination of organisms after field application can be expected to differ based on the high-water content of swine manure compared to cattle manure.…”

Section: Introductionmentioning

confidence: 99%

Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water

et al. 2022

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Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked resistance genes from source manures and identified important differences in antimicrobial resistance gene transport and enrichment over time in the soil and water of artificially drained cropland. The source manures had distinct microbial community and resistance gene profiles, and these differences were also reflected in the soil columns after manure application. Antibiotic resistance genes (ARGs) were only significantly enriched in effluent samples following the first rain event (day 11) for both soil types compared to the control columns, illustrating the high background level of resistance present in the control soils chosen. For swine, the genes tetQ, tet(36), tet44, tetM, sul2 and ant(6)-ib persisted in the soil columns, whereas tetO, strB and sul1 persisted in effluent samples. Conversely, for cattle manure sul2 and strB persisted in both soil and effluent. The distinct temporal dynamics of ARG distribution between soil and effluent water for each manure type can be used to inform potential mitigation strategies in the future.

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Microbial community competition rather than high-temperature predominates ARGs elimination in swine manure composting

Cited by 63 publications

References 31 publications

Effects of Oxytetracycline/Lead Pollution Alone and in the Combined Form on Antibiotic Resistance Genes, Mobile Genetic Elements, and Microbial Communities in the Soil

Effects of Oxytetracycline/Lead Pollution Alone and in the Combined Form on Antibiotic Resistance Genes, Mobile Genetic Elements, and Microbial Communities in the Soil

Response behavior of antibiotic resistance genes to zinc oxide nanoparticles in cattle manure thermophilic anaerobic digestion process: A metagenomic analysis

Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water

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