Microbial Community Composition in the Marine Sediments of Jeju Island: Next-Generation Sequencing Surveys

Abstract

Marine sediments are a microbial biosphere with an unknown physiology, and the sediments harbor numerous distinct phylogenetic lineages of Bacteria and Archaea that are at present uncultured. In this study, the structure of the archaeal and bacterial communities was investigated in the surface and subsurface sediments of Jeju Island using a next-generation sequencing method. The microbial communities in the surface sediments were distinct from those in the subsurface sediments; the relative abundance of sequences for Thaumarchaeota, Actinobacteria, Bacteroides, Alphaproteobacteria, and Gammaproteobacteria were higher in the surface than subsurface sediments, whereas the sequences for Euryarchaeota, Acidobacteria, Firmicutes, and Deltaproteobacteria were relatively more abundant in the subsurface than surface sediments. This study presents detailed characterization of the spatial distribution of benthic microbial communities of Jeju Island and provides fundamental information on the potential interactions mediated by microorganisms with the different biogeochemical cycles in coastal sediments.

Marine bacterial communities in the upper gulf of Thailand assessed by Illumina next-generation sequencing platform

“…This result corresponds with that of Suh et al [7] who reported that the Proteobacteria was the dominant phylum in seawater from Gosung Bay, South Korea, followed by the Bacteroidetes and Actinobacteria. Similarly, most of the bacterial sequence reads in marine sediments from Jeju Island, South Korea, were also associated with the Proteobacteria and Bacteroidetes, followed by the Actinobacteria, Acidobacteria, and Firmicutes [20].…”

“…Marine environment is the one of the most extensive habitats for microorganisms, covering more than twothirds of the surface of the earth [20]. Marine bacteria play important roles in energy and matter fluxes in the sea [7].…”

“…The considerably more OTUs were observed in marine sediments. Totals of 6039 OTUs, 6059 OTUs, and 5700 to 7600 OTUs were obtained from marine sediments around the Kaichu-Doro Causeway in Okinawa, Japan [31], marine sediments in Yam O Wan Bay, Hong Kong [32], and marine sediments from Jeju Island, South Korea [20], respectively. Moreover, this study found that the proportions of the Proteobacteria were highest in all sampling sites, followed by the Bacteroidetes and Actinobacteria.…”

Shifts in coastal sediment oxygenation cause pronounced changes in microbial community composition and associated metabolism

“…This use of alternative electron acceptors in turn influences the oxygen-reduction potential at various depths in the sediment. Analysis of sediments from around the world has identified key microbial taxa associated with these redox reactions [ 13 – 15 ]. The microbial reduction of Fe 3+ and SO 4 2− produces ferrous iron (Fe 2+ ) and toxic H 2 S, respectively, that can subsequently chemically precipitate as iron sulfides.…”

Ecological and biotechnological aspects of Aplysina-associated microorganisms

“…Firmicutes have been associated to diseased specimen of A. aerophoba (Webster, Xavier, et al, 2008) and significantly changed in their relative abundance after transplantation of sponge specimens (Meyer et al, 2016). More commonly however, Clostridia represent dominant members of the microbial communities inhabiting marine sediments (Choi et al, 2016) and might thus be only coincidentally present in sponges at the time of sampling. The spore-forming capabilities of this generally obligate anaerobic lineage (Yutin & Galperin, 2013) probably rendered Clostridia resistant to oxygen during sponge processing and cryopreservation and enabled their growth in the MiniColumns once the oxygen was depleted.…”