Microbial community dynamics in anaerobic digesters treating conventional and vacuum toilet flushed blackwater

Gao, Mengjiao; Guo, Bing; Zhang, Lei; Zhang, Yingdi; Liu, Yang

doi:10.1016/j.watres.2019.05.077

Cited by 79 publications

(30 citation statements)

References 51 publications

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“…Thus, it can be speculated that the members of suspended microbial communities were mainly originated from the feedstock. Families from the order Bacteroidales (e.g., Porphyromonadaceae and Bacteroidaceae ) are well‐known for their cellulose hydrolysis capability (Ozbayram, Akyol, Ince, Karakoç, & Ince, 2018; Westerholm & Schnürer, 2019; Xia et al., 2014), which associated with their high abundance in the blackwater‐fed reactors containing lignocellulosic matter (Gao, Guo, et al, 2019). The high abundance of Tissierellaceae , Ruminococcaceae , and families from the order Clostridiales (e.g., Clostridiaceae ) was also detected in both biofilms and suspension.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, species belonging to these families have been frequently found in high‐ammonia stressed anaerobic communities (Müller, Sun, Westerholm, & Schnürer, 2016). Clostridiales species were reported to be tolerant to high‐ammonia concentrations (De Vrieze et al., 2015; Gao, Guo, et al, 2019). Also, species belong to family Tissierellaceae were known for high tolerance to salts, such as Tissierella and Soehngeni (Wang, Hou, & Su, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…The highest organic loading rate (OLR) of 4.87 g COD/L‐d for blackwater mono‐digestion so far was achieved in a mesophilic UASB reactor (Gao, Zhang, Guo, Zhang, & Liu, 2019). However, due to low biodegradability of blackwater as well as the potential of free ammonia inhibition (Florentino, Sharaf, Zhang, & Liu, 2019; Gao, Zhang, Florentino, & Liu, 2018), only few studies could achieve OLRs higher than 1.0 g COD/L‐d (Abdel‐Shafy, El‐Khateeb, Regelsberger, El‐Sheikh, & Shehata, 2009; Moges et al., 2018; van Voorthuizen, Zwijnenburg, van der Meer, & Temmink, 2008), while mostly ranged from 0.3 to 1.0 g COD/L‐d (De Graaff et al., 2010; Gao, Guo, Zhang, Zhang, & Liu, 2019; Kujawa‐Roeleveld, Elmitwalli, & Zeeman, 2006; Luostarinen et al., 2007; Meulman, Zeeman, & Buisman, 2008; Tervahauta, Bryant, Leal, Buisman, & Zeeman, 2014; Wendland et al., 2007; Zamalloa et al., 2013). Blackwater is mostly discharged at ambient temperatures, while digestion efficiency could be further limited by low hydrolysis rate and low methanogenic activity without heating (Zhang, Hendrickx, Kampman, Temmink, & Zeeman, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A high‐rate anaerobic biofilm reactor for biomethane recovery from source‐separated blackwater at ambient temperature

Huang

Zakaria

Zhang

et al. 2020

Water Environment Research

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Anaerobic bioreactors for source-separated blackwater are mostly operated at low organic loading rates (OLRs) due to low biodegradability and the potential of ammonia inhibition. In this study, an anaerobic biofilm reactor having conductive carbon fibers as the media was investigated for the high-rate treatment of blackwater collected from vacuum toilets. The bioreactor was operated at different OLRs ranged from 0.77 to 3.01 g COD/L-d in four stages for a total operating period of ~ 250 days. With the increase of OLRs, the specific methane production rate increased from 105.3 to 304.6 ml/L-d with high methane content in biogas (75.5%-83%). The maximum methane yield was achieved at hydraulic retention time (HRT) of 15 days. Highest organics and suspended solids removal (80%-83%) were achieved at 20-days HRT, while increased OLRs resulted in diminished removal efficiencies. The state variables, including pH, total ammonia nitrogen, short-chain volatile fatty acids, and soluble chemical oxygen demand, indicated the system had a great capability to withstand the high OLRs. Microbial community analysis revealed that the high performance might be attributed to direct interspecies electron transfer (DIET) facilitated by potentially electroactive bacteria (e.g., Syntrophomonas, Clostridium) and electrotrophic archaea (e.g., Methanosaeta and Methanosarcina species) enriched on the carbon fibers. © 2020 Water Environment Federation • Practitioner points • An anaerobic biofilm reactor was investigated for biomethane recovery from sourceseparated blackwater. • Conductive carbon fibers were utilized as the media to stimulate enrichment of potentially electroactive methanogenic communities. • The bioreactor was operated at ambient temperature for over 250 days. • High methane production rate and high-quality biogas were achieved at OLRs ranged from 0.77 to 3.01 g COD/L-d. • Microbial community analysis suggested direct interspecies electron transfer (DIET) between specific electroactive bacteria and electrotrophic archaea.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A high‐rate anaerobic biofilm reactor for biomethane recovery from source‐separated blackwater at ambient temperature

Huang

Zakaria

Zhang

et al. 2020

Water Environment Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…The good COD removal was maintained despite the fluctuating OLR, coupled with a moderate E. coli (1.96 log) and Total Coliforms (2.13 log) removal [ 112 ]. In a coherent way, a recent notable research proved that different blackwater types (coming from conventional and vacuum toilets) enriched distinct microbial consortia in UASB treatment, probably due to the diverse sulfate and ammonia loadings [ 113 ].…”

Section: Uasb Treatment Of Municipal Wastewatermentioning

confidence: 99%

Up-Flow Anaerobic Sludge Blanket (UASB) Technology for Energy Recovery: A Review on State-of-the-Art and Recent Technological Advances

2020

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Up-flow anaerobic sludge blanket (UASB) reactor belongs to high-rate systems, able to perform anaerobic reaction at reduced hydraulic retention time, if compared to traditional digesters. In this review, the most recent advances in UASB reactor applications are critically summarized and discussed, with outline on the most critical aspects for further possible future developments. Beside traditional anaerobic treatment of soluble and biodegradable substrates, research is actually focusing on the treatment of refractory and slowly degradable matrices, thanks to an improved understanding of microbial community composition and reactor hydrodynamics, together with utilization of powerful modeling tools. Innovative approaches include the use of UASB reactor for nitrogen removal, as well as for hydrogen and volatile fatty acid production. Co-digestion of complementary substrates available in the same territory is being extensively studied to increase biogas yield and provide smooth continuous operations in a circular economy perspective. Particular importance is being given to decentralized treatment, able to provide electricity and heat to local users with possible integration with other renewable energies. Proper pre-treatment application increases biogas yield, while a successive post-treatment is needed to meet required effluent standards, also from a toxicological perspective. An increased full-scale application of UASB technology is desirable to achieve circular economy and sustainability scopes, with efficient biogas exploitation, fulfilling renewable energy targets and green-house gases emission reduction, in particular in tropical countries, where limited reactor heating is required.

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“…Whereas, the presence of Chloroflexi has been negatively correlated with VFAs production, other phylum such as Firmicutes prevails in environments devoted for VFAs production [11,33]. SAOB are affiliated with Firmicutes phylum, more particularly to Clostridia class (Thermacetogenium phaeum, Tepidanaerobacter acetatoxydans, or Syntrophaceticus schinkii), Tissierellia class (Clostridium ultunense) and Thermotogae phylum (Pseudothermotoga lettingae) [34,35]. However, other members of Firmicutes have been attributed to perform SAO activities.…”

Section: Microbial Population Dynamicsmentioning

confidence: 99%

Volatile Fatty Acids Production from Microalgae Biomass: Anaerobic Digester Performance and Population Dynamics during Stable Conditions, Starvation, and Process Recovery

2019

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Disturbances in anaerobic digestion (AD) negatively impact the overall reactor performance. These adverse effects have been widely investigated for methane generation. However, AD recently appeared as a potential technology to obtain volatile fatty acids (VFAs) and thus, the impact of process disturbances must be evaluated. In this sense, microbial response towards a starvation period of two weeks was investigated resulting in a conversion of organic matter into VFAs of 0.39 ± 0.03 COD-VFAs/CODin. However, the lack of feeding reduced the yield to 0.30 ± 0.02 COD-VFAs/CODin. Microbial analysis revealed that the starvation period favored the syntrophic acetate-oxidizing bacteria coupled with hydrogenotrophic methanogens. Finally, the system was fed at 9 g COD/Ld resulting in process recovery (0.39 ± 0.04 COD-VFAs/CODin). The different microbiome obtained at the end of the process was proved to be functionally redundant, highlighting the AD robustness for VFAs production.

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Microbial community dynamics in anaerobic digesters treating conventional and vacuum toilet flushed blackwater

Cited by 79 publications

References 51 publications

A high‐rate anaerobic biofilm reactor for biomethane recovery from source‐separated blackwater at ambient temperature

A high‐rate anaerobic biofilm reactor for biomethane recovery from source‐separated blackwater at ambient temperature

Up-Flow Anaerobic Sludge Blanket (UASB) Technology for Energy Recovery: A Review on State-of-the-Art and Recent Technological Advances

Volatile Fatty Acids Production from Microalgae Biomass: Anaerobic Digester Performance and Population Dynamics during Stable Conditions, Starvation, and Process Recovery

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