Microbial community structure and diversity in a native forest wood-decomposed hollow-stump ecosystem

Abstract

The aim of this study is to investigate the microbial community structure and diversity in a wooddecomposed hollow-stump ecosystem. Microbial communities of SD-1, a lateritic soil sample from forest hollowstump ecosystems in Fuzhou (a southeastern coast city of China), were characterized by constructing and analyzing rRNA gene clone libraries. Sixty-six phylotypes were identified from 112 bacterial clones, including Acidobacteria (71.5%), Proteobacteria (24.1%) and Verrucomicrobia (0.9%). A total of 40 phylotypes were obtained from 138 fungal clones, including Basidiomycota (42.8%), Ascomycota (36.2%), Zygomycota (13.8%), Chytridiomycota (2.9%) and Fungi incertae sedis (4.3%). The results showed a variety of clones related to the reported lignocellulosedecomposing microorganisms. They included some important bacterial decomposers, such as Sphingomonas and Burkholderia, and a number of wood-decaying fungi, including Tricholomataceae, Strophariaceae and Agaricaceae of Basidiomycota; Orbilia, Aspergillus, Phialocephala, Epicoccum and Phoma of Ascomycota and Mucorales of Zygomycota. The result indicated that the lignocellulolytic microorganisms worked synergically with a unique community structure to biodegrade lignocellulose in the hollowstump ecosystem.

Diversity and structure of bacterial communities associated with Phanerochaete chrysosporium during wood decay

“…The second important OTU (OTU 1025), showing a mean relative abundance of 4.3% throughout all microcosms (B‐BF core), belonged to the Rhizobium genus. It has been found in archaeological waterlogged wood (Landy et al ., ), in the gastrointestinal tract of wood‐eating catfish (McDonald et al ., ) and in decaying wood in forest (Zhang et al ., ; Tian et al ., ), confirming its association with the decayed wood niche. Because nitrogen is a limiting factor for the biology of wood decaying fungi, it has been suggested that nitrogen‐fixing bacteria, like Rhizobia (Dreyfus et al ., ), could possibly counterbalance the wood nitrogen deficiency (Clausen, ).…”

Structural and functional variation in soil fungal communities associated with litter bags containing maize leaf

“…Using such methods, much greater depth of coverage can be achieved in rRNA gene-based surveys (Roesch et al, 2007;Lauber et al, 2009). The application of high-throughput sequencing strategies has recently greatly expanded our appreciation of fungal diversity in soil habitats (Buee et al, 2009;Lim et al, 2010;Tian et al, 2010;Dumbrell et al, 2011;Verbruggen et al, 2012). Furthermore, the development of shot-gun metagenomic and metatranscriptomic approaches has afforded a new perspective into the functional capacities and expressed activities, respectively, in complex microbial communities (Falkowski et al, 2008;DeLong, 2009).…”

Potential of selected fungi for biological stump removal of Eucalyptus spp.

“…Among the fungi with this potential, wood-decaying fungi present the greatest potential for use. Known as white-, brown-and soft-rot fungi, they are responsible for the majority of wood decay and use enzymatic and non-enzymatic systems to degrade cellulose, hemicellulose and lignin of the wood cell wall (Zabel and Morrell, 1992;Schmidt, 2006;Tian et al, 2010;Ryss et al, 2011;Bari et al, 2015).…”