Microbial community structure and function during abnormal curve development of substrate-induced respiration measurements

ETBE (ethyl tert butyl ether) and TAME (tert amyl methyl ether) affect microbial community structure and function in soils

“…The lag-time of microbial respiration (defined as time from substrate addition to start of exponential growth according to ISO 17155 [24]) may also reflect the quality of the previously available carbon source [31]. This is confirmed by the terrace formation (visible after the peak maximum in the TAME and ETBE related respiration curves), since that phenomenon has been linked to the utilization of an additive as additional carbon source in a previous study [28]. Furthermore, ETBE is considered to be more easily degradable compared to TAME, which could explain the differences in the respiration curve progressions, the early start of exponential growth and the higher respiration maximum [32][33][34][35].…”

“…the exponential slope in the respiration curve as defined in ISO 17155 [24]) started later in the contaminated soil samples at the beginning of incubation. It has been reported that prolonged lag-times may indicate a toxic effect of chemicals on the microbial communities [28][29][30]. The lag-time of microbial respiration (defined as time from substrate addition to start of exponential growth according to ISO 17155 [24]) may also reflect the quality of the previously available carbon source [31].…”

Physiological Responses of a Diazotrophic Cyanobacterium to Acidification of Paddy Floodwater: N2 Fixation, Photosynthesis, and Oxidative–Antioxidative Characteristics

“…Activities of respiration and photosynthesis could reflect the vitality of microorganisms [ 25 , 26 ]. The respiration rate of YYLX235 in the acidified treatments (pH 6.0–4.0) was far lower than the neutral control, indicating that the acidification of paddy floodwater decreased cell vitality of this diazotrophic cyanobacterium.…”

Microbial Biomass

“…[ Brant et al, 2006] 13 CO2 Desert soil PLFAs Fungal-to-bacterial PLFA-C increased under elevated CO2 [Jin and Evans, 2010] [Kindler et al, 2009] 13 C-methanotrophs Rice field soil PLFAs Eukaryotes, probably protozoa, preferentially graze on methanotrophs [Murase et al, 2011] As can be seen from Table 1 these studies include both laboratory and field experiments and a wide range of 13 C-labelled substrates. Dynamic-SIP approaches can be divided into two broad categories, namely those applying: (i) complex substrates, such as 13 C-labelled plant litter [Williams et al, 2006;Moore-Kucera and Dick, 2008;Herman et al, 2012;Gan et al, 2013;Helgason et al, 2014;Creamer et al, 2015;Bai et al, 2016] or 13 C-lablled microbial cultures [Kindler et al, 2006[Kindler et al, , 2009Murase et al, 2011], or (ii) pure 13 C-labelled compounds [Brant et al, 2006;Bartling et al, 2009;Mellendorf et al, 2010;Jakobs-Schonwandt et al, 2010;Dungait et al, 2011;Churchland et al, 2012;Apostel et al, 2013;Lemanski and Scheu, 2014]. 13 CO2 pulse-chase experiments are amongst the most widely applied, designed to deliver 13 C-labelled root exudate derived from newly formed 13 C-photosynthate to explore C allocation from plants to the below-ground biomass [Leake et al, 2006;Jin and Evans, 2010;Hogberg et al, 2010;Ladygina and Hedland, 2010;Hannula et al, 2012;Yao et al, 2012;Dirgo et al, 2013;Tavi et al, 2013;Bahn et al, 2013;Dias et al, 2013;Balasooriya et al, 2014;Malik et al,...…”