Microbial community structure in the rhizosphere of Sophora viciifolia grown at a lead and zinc mine of northwest China

Xu, Zhouying; Tang, Ming; Chen, H.; Ban, Yihui; Zhang, H.H.

doi:10.1016/j.scitotenv.2012.07.029

Cited by 55 publications

(44 citation statements)

References 73 publications

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“…This region is in the warm temperate semiarid zone with an annual average temperature of 11.4 • C. The annual average rainfall is 613.2 mm with a frost-free period of 188 days. The soil texture was classified as cinnamon and brunisolic soil, according to the traditional soil genesis classification standard in China [27]. Qiandongshan Pb-Zn region is the largest and the most typical five Pb and Zn producers in China, and its total reserve is approximately 1.12 × 10 6 tons [28].…”

Section: Study Area and Samplingmentioning

confidence: 99%

“…The HM polluted area had the same soil type and was divided into polluted levels of light (S1, 33 • Figure S1), according to our previous investigation and the Chinese environmental quality standard (Grade II) for soils (GB 15618-1995) [27,[29][30][31]. In August 2012, four study plots (15 × 15 m) dominated by target tree species (R. pseudoacacia) were identified at each site for soil sampling.…”

Section: Study Area and Samplingmentioning

confidence: 99%

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Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Yang

Dong

Cao

et al. 2017

Forests

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Abstract:The toxic effects of heavy metal (HM) contamination on plant metabolism and soil microorganisms have been emphasized recently; however, little is known about the differences in soil physical, chemical, and biological properties between bulk and rhizosphere soils contaminated with HMs in forest ecosystem. The present study was conducted to evaluate the rhizosphere effect on soil properties, enzyme activities and bacterial communities associated with Robinia pseudoacacia L. along a HM contamination gradient. Soil organic matter (SOM), available nitrogen (AN) and phosphorus (AP) contents were significantly higher in rhizosphere soil than those in bulk soil at HM contaminated sites (p < 0.05). Compared to bulk soil, activities of four soil enzymes indicative of C cycle (β-glucosidase), N cycle (protease, urease) and P cycle (alkaline phosphatase) in rhizosphere soil across all study sites increased by 47.5%, 64.1%, 52.9% and 103.8%, respectively. Quantitative PCR (qPCR) and restriction fragment length polymorphism (RFLP) were used to determine the relative abundance, composition and diversity of bacteria in both bulk and rhizosphere soils, respectively. The copy number of bacterial 16S rRNA gene in bulk soil was significantly lower than that in rhizosphere soil (p < 0.05), and it had significantly negative correlations with total/DTPA-extractable Pb concentrations (p < 0.01). Alphaproteobacteria, Gammaproteobacteria and Firmicutes were the most dominant groups of bacteria at different study sites. The bacterial diversity index of Species richness (S) and Margalef (d Ma ) were significantly higher in rhizosphere soil compared with those in bulk soil, although no difference could be found in Simpson index (D) between bulk and rhizosphere soils (p > 0.05). Redundancy analysis (RDA) results showed that soil pH, EC, SOM and total/DTPA-extractable Pb concentrations were the most important variables affecting relative abundance, composition and diversity of bacteria (p < 0.05). Our study highlights the importance of rhizosphere effect on soil nutrient content, enzyme activity, bacterial abundance and community in HM contaminated forest soils. Further study is still required to understand the specific processes in the rhizosphere to achieve a suitable rhizosphere biotechnology for restoration of degraded forest ecosystem.

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Section: Study Area and Samplingmentioning

confidence: 99%

Section: Study Area and Samplingmentioning

confidence: 99%

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Yang

Dong

Cao

et al. 2017

Forests

View full text Add to dashboard Cite

show abstract

“…The population of resistant bacteria comprised less than 2 % of the indigenous microbial communities. However, the number of resistant bacteria can increase in contaminated soils, reaching 10 % or even 100 %, as the contaminants greatly change in terms of quantity and composition (Xu et al 2012). Moreover, these bacteria serve a significant function in maintaining the ecological balance of the contaminated soil and could be used for the bioremediation of metal-polluted soil (Hayat et al 2010;Kandeler et al 2000).…”

Section: Bacteria With Potential Resistance To Heavy Metalsmentioning

confidence: 99%

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

Chen

Xing

et al. 2015

Environ Sci Pollut Res

196

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Mine activities leaked heavy metals into surrounding soil and may affected indigenous microbial communities. In the present study, the diversity and composition of the bacterial community in soil collected from three regions which have different pollution degree, heavy pollution, moderate pollution, and non-pollution, within the catchment of Chao River in Beijing City, were compared using the Illumina MiSeq sequencing technique. Rarefaction results showed that the polluted area had significant higher bacterial alpha diversity than those from unpolluted area. Principal component analysis (PCA) showed that microbial communities in the polluted areas had significant differences compared with the unpolluted area. Moreover, PCA at phylum level and Matastats results demonstrated that communities in locations shared similar phyla diversity, indicating that the bacterial community changes under metal pollution were not reflected on phyla structure. At genus level, the relative abundance of dominant genera changed in sites with degrees of pollution. Genera Bradyrhizobium, Rhodanobacter, Reyranella, and Rhizomicrobium significantly decreased with increasing pollution degree, and their dominance decreased, whereas several genera (e.g., Steroidobacter, Massilia, Arthrobacter, Flavisolibacter, and Roseiflexus) increased and became new dominant genera in the heavily metal-polluted area. The potential resistant bacteria, found within the genera of Thiobacillus, Pseudomonas, Arthrobacter, Microcoleus, Leptolyngbya, and Rhodobacter, are less than 2.0 % in the indigenous bacterial communities, which play an important role in soil ecosystem. This effort to profile the background diversity may set the first stage for better understanding the mechanism underlying the community structure changes under in situ mild heavy metal pollution.

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“…Nested PCR was used to increase the resolution yield of DGGE [17][18][19]. The highly variable V3 region of the bacterial 16S rRNA gene was amplified using the fD1 and rP1 primer pair in the first round of PCR and the 341F-GC and 534R primer pair in the second round [20].…”

Section: Nested Pcrmentioning

confidence: 99%

“…in which S is the number of bands in a lane, N i is the peak density of the ith band and N is the total peak density of all of the bands in a lane [17][18][19]. Significant differences between means were analyzed using the t-test in SPSS Version 19.0.…”

Section: Dgge Band Profile Analysismentioning

confidence: 99%

Influence of Starvation on the Structure of Gut-Associated Bacterial Communities in the Chinese White Pine Beetle (Dendroctonus armandi)

Zhang

et al. 2016

Forests

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This study investigated the influence of starvation on the structure of the gut bacterial community in the Chinese white pine beetle (Dendroctonus armandi). A total of 14 operational taxonomic units (OTUs 0.03 ) clusters belonging to nine genera were identified. Denaturing gradient gel electrophoresis (DGGE) profiles of bacterial PCR-amplified 16S rRNA gene fragments from the guts of starved male and female adults revealed that the bacterial community diversity increased after starvation. The dominant genus Citrobacter decreased significantly, whereas the genus Serratia increased in both starved female and starved male adults. The most predominant bacterial genus in D. armandi adults was Citrobacter, except for starved male adults, in which Serratia was the most abundant genus (27%). Our findings reveal that starvation affects gut bacterial dynamics in D. armandi, as has been observed in other insect species.

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Microbial community structure in the rhizosphere of Sophora viciifolia grown at a lead and zinc mine of northwest China

Cited by 55 publications

References 73 publications

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

Influence of Starvation on the Structure of Gut-Associated Bacterial Communities in the Chinese White Pine Beetle (Dendroctonus armandi)

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