Microbial community succession, species interactions and metabolic pathways of sulfur-based autotrophic denitrification system in organic-limited nitrate wastewater

Han, Fang; Zhang, Mengru; Shang, Hongguo; Liu, Zhe; Zhou, Weizhi

doi:10.1016/j.biortech.2020.123826

Cited by 126 publications

(12 citation statements)

References 41 publications

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Section: Changes In Microbial Community Structuresupporting

confidence: 86%

“…While in the domesticated sludge samples A2 and B2, the abundance of Proteobacteria increased to 89.65% and 40.68%, respectively, and the proportion of B2 declined by 54.6% (compared with A2) when influent NO − 3 -N concentration decreased from 960 to 10.95 mg/L. Proteobacteria has been proved to be the main phylum of autotrophic denitrifiers [22], therefore, their high abundance was consistent with the previously derived dominant role of sulfur autotrophic denitrification. Proteobacteria could survive under high/low nitrate trophic conditions and their growth may be stimulated with the potential to resist environmental stress.…”

Section: Changes In Microbial Community Structurementioning

confidence: 96%

“…The fraction of nitrate removed by heterotrophic denitrification might be lower than that of the autotrophic denitrification, and microbes may affect the utilization of COD. Moreover, some organic matter can also be provided from microbial products and degraded biofilms [22].…”

Section: Nitrogen Removal Performances Under Different Trophic Condit...mentioning

confidence: 99%

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Nitrate Removal from Actual Wastewater by Coupling Sulfur-Based Autotrophic and Heterotrophic Denitrification under Different Influent Concentrations

Liu

Wang

Zhang

et al. 2021

Water

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Contamination of wastewater with organic-limited nitrates has become an urgent problem in wastewater treatment. The cooperating heterotrophic with sulfur autotrophic denitrification is an alternative process and the efficiency has been assessed in many studies treating simulated wastewater under different operating conditions. However, due to the complex and diverse nature of actual wastewater, more studies treating actual wastewater are still needed to evaluate the feasibility of collaborative denitrification. In this study, lab-scale experiments were performed with actual nitrate polluted water of two different concentrations, with glucose and sodium thiosulfate introduced as mixed electron donors in the coupling sulfur-based autotrophic and heterotrophic denitrification. Results showed that the optimum denitrification performance was exhibited when the influent substrate mass ratio of C/N/S was 1.3/1/1.9, with a maximum denitrification rate of 3.52 kg NO3−-N/(m3 day) and nitrate removal efficiency of 93% in the coupled systems. Illumina high-throughput sequencing analysis revealed that autotrophic, facultative, and heterotrophic bacteria jointly contributed to high nitrogen removal efficiency. The autotrophic denitrification maintained as the predominant process, while the second most prevalent denitrification process gradually changed from heterotrophic to facultative with the increase of influent concentration at optimum C/N/S ratio conditions. Furthermore, the initiation of dissimilatory nitrate reduction to ammonium (DNRA) was very pivotal in promoting the entire denitrification process. These results suggested that sulfur-based autotrophic coupled with heterotrophic denitrifying process is an alternative and promising method to treat nitrate containing wastewater.

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Section: Changes In Microbial Community Structuresupporting

confidence: 86%

Section: Changes In Microbial Community Structurementioning

confidence: 96%

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Nitrate Removal from Actual Wastewater by Coupling Sulfur-Based Autotrophic and Heterotrophic Denitrification under Different Influent Concentrations

Liu

Wang

Zhang

et al. 2021

Water

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“…Cron et al (2019) suggested that sulfur-oxidizing bacteria (Sulfuricurvum kujiense, also detected in this study) could use soluble organics to stabilize stores of bioavailable S 0 outside the cells. Recent bioreactor study reported key functional heterotrophic and autotrophic denitrifiers jointly contributed to high nitrogen removal efficiency by symbiotic relationships (Han et al, 2020). Although we did not measure organic C dynamics in this study, these symbiotic relationships may also occur in natural subsoil with lower available organic C in the study area.…”

Section: Sulfur-based Denitrification Using Inherent S In Subsoil In Marine Sedimentary Rock Regionsmentioning

confidence: 77%

Sulfur-Based Denitrification in Streambank Subsoils in a Headwater Catchment Underlain by Marine Sedimentary Rocks in Akita, Japan

Hayakawa

Ota

Asano

et al. 2021

Front. Environ. Sci.

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Sulfur-based denitrification may be a key biogeochemical nitrate (NO3−) removal process in sulfide-rich regions, but it is still poorly understood in natural terrestrial ecosystems. We examined sulfur-driven NO3− reduction using streambank soils in a headwater catchment underlain by marine sedimentary rock in Akita, Japan. In a catchment exhibiting higher sulfide content in streambed sediment, we sampled two adjacent streambank soils of streambank I (two layers) and of streambank II (eight layers). Anaerobic long-term incubation experiments (40 days, using soils of streambank I) and short-term incubation experiments (5 days, using soils of streambank II) were conducted to evaluate variations of N solutes (NO3−, NO2−, and NH4+), N gases (NO, N2O), and the bacterial flora. In both experiments, two treatment solutions containing NO3− (N treatment), and NO3− and S2O32− (N + S treatment) were prepared. In the N + S treatment of the long-term experiment, NO3− concentrations gradually decreased by 98%, with increases in the SO42−, NO2−, NO, and N2O concentrations and with not increase in the NH4+, indicating denitrification had occurred with a high probability. Temporal accumulation of NO2− was observed in the N + S treatment. The stoichiometric ratio of SO42− production and NO3− depletion rates indicated that denitrification using reduced sulfur occurred even without additional S, indicating inherent S also served as an electron donor for denitrification. In the short-term incubation experiment, S addition was significantly decreased NO3− concentrations and increased NO2−, NO, and N2O concentrations, especially in some subsoils with higher sulfide contents. Many denitrifying sulfur-oxidizing bacteria (Thiobacillus denitrificans and Sulfuricella denitrificans) were detected in both streambank I and II, which dominated up to 5% of the entire microbial population, suggesting that these bacteria are widespread in sulfide-rich soil layers in the catchment. We concluded that the catchment with abundant sulfides in the subsoil possessed the potential for sulfur-driven NO3− reduction, which could widely influence N cycling in and NO3− export from the headwater catchment.

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“…The autotrophs and heterotrophs were both abundant in S 1 –S 4 . The heterotrophs in S 1 –S 4 , including Ferruginibacter , Bacteroides , Trichococcus , Dokdonella , Herminiimonas , Sphingobium , and Cellulomonas , are associated with the degradation of organics or HD processes ( Chen et al, 2016 ; Han et al, 2020 ; Tian et al, 2021 ; Xin and Qiu, 2021 ; Zhang et al, 2021 ), and their abundances were both decreased or disappeared in S 5 –S 8 . The abundance of Rhodanobacter increased in R 1 and R 3 reactors, even though these bacteria are related to the degradation of carbohydrates ( Xin and Qiu, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Denitrification Performance in Packed-Bed Reactors Using Novel Carbon-Sulfur-Based Composite Filters for Treatment of Synthetic Wastewater and Anaerobic Ammonia Oxidation Effluent

Wang

Liang²,

Kang³

et al. 2022

Front. Microbiol.

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To avoid nitrate pollution in water bodies, two low-cost and abundant natural organic carbon sources were added to make up the solid-phase denitrification filters. This study compared four novel solid-phase carbon-sulfur-based composite filters, and their denitrification abilities were investigated in laboratory-scale bioreactors. The filter F4 (mixture of elemental sulfur powder, shell powder, and peanut hull powder with a mass ratio of 6:2.5:1.5) achieved the highest denitrification ability, with an optimal nitrate removal rate (NRR) of 723 ± 14.2 mg NO3–-N⋅L–1⋅d–1 when the hydraulic retention time (HRT) was 1 h. The HRT considerably impacted effluent quality after coupling of anaerobic ammonium oxidation (ANAMMOX) and solid-phase-based mixotrophic denitrification process (SMDP). The concentration of suspended solids (SS) of the ANAMMOX effluent may affect the performance of the coupled system. Autotrophs and heterotrophs were abundant and co-existed in all reactors; over time, the abundance of heterotrophs decreased while that of autotrophs increased. Overall, the SMDP process showed good denitrification performance and reduced the sulfate productivity in effluent compared to the sulfur-based autotrophic denitrification (SAD) process.

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Microbial community succession, species interactions and metabolic pathways of sulfur-based autotrophic denitrification system in organic-limited nitrate wastewater

Cited by 126 publications

References 41 publications

Nitrate Removal from Actual Wastewater by Coupling Sulfur-Based Autotrophic and Heterotrophic Denitrification under Different Influent Concentrations

Nitrate Removal from Actual Wastewater by Coupling Sulfur-Based Autotrophic and Heterotrophic Denitrification under Different Influent Concentrations

Sulfur-Based Denitrification in Streambank Subsoils in a Headwater Catchment Underlain by Marine Sedimentary Rocks in Akita, Japan

Denitrification Performance in Packed-Bed Reactors Using Novel Carbon-Sulfur-Based Composite Filters for Treatment of Synthetic Wastewater and Anaerobic Ammonia Oxidation Effluent

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