Microbial content of house dust samples determined with qPCR

Kaarakainen, Pasi; Rintala, Helena; Vepsäläinen, Asko; Hyvärinen, Anne; Nevalainen, Aino; Meklin, Teija

doi:10.1016/j.scitotenv.2009.04.046

Cited by 73 publications

(65 citation statements)

References 29 publications

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“…The results of the present investigation has shown that dust samples collected from carpets contained several types of bacteria including S. aureus, S. epidermidis, Gram negative bacilli, and other types of molds such as Asperigillus and Penicillum spp. and others which is in agreement with the findings by other researchers [27]. It has also been reported that aerosolization of carpet dust can be a source to spread other pathogenic bacteria such as Salmonella and Clostridium botulinum [28].…”

Section: IVsupporting

confidence: 92%

Bacterial and Fungal Contamination of Air conditioners filters and Carpets

Almoffarreh¹,

Alsaleh²,

Alruwaili³

2016

IJEAB

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Section: IVsupporting

confidence: 92%

Bacterial and Fungal Contamination of Air conditioners filters and Carpets

Almoffarreh¹,

Alsaleh²,

Alruwaili³

2016

IJEAB

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“…(Myco). These were analyzed using quantitative polymerase chain reaction (qPCR) techniques [7]. Allergen levels were determined using two-site sandwich ELISA for house dust mite (Der p1, Der f1), cat (Fel d1), dog (Can f1) while horse allergen (Equ cx) was measured using monoclonal antibodies [8] [9].…”

Section: Microbiology and Allergensmentioning

confidence: 99%

The Impact of Microbiological Pollutants on School Indoor Air Quality

Fsadni¹,

Frank²,

Fsadni³

et al. 2017

GEP

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Asthma is common in children with allergens and mould influencing the development of the disease. Since children spend most of their time outside their homes within the school environment, school indoor air quality can directly influence their respiratory health. This study aims to identify microbiological contaminants in Maltese schools. The association between contaminants, respiratory health and school characteristics has been analysed. Five primary schools were selected with 9 to 11 years old students participating. Standardised health questionnaires, lung function tests, and school characterisation were performed. Dust samples were analysed for fungi, bacteria and allergens were performed. Penicillium/Aspergillus/Paecilomyces/Variotii (PenAsp) group had the highest median indoor concentration followed by Mycobacterial and Streptomyces species. There was a significant negative correlation between PenAsp and Mycobacterium spp levels in all the participating schools (r = −0.42; p = 0.03). Cat allergen in classroom dust correlated positively with the number of cat owners (r = 0.43; p = 0.041). High exposure to fungi, bacteria and allergens was significantly associated with upper and lower airway atopy. School/classroom characteristics and cleaning protocols were significantly associated with exposure to these pollutants. In conclusion, fungi, bacteria, endotoxin, cat and dog allergens have been found to have a direct influence on school indoor air quality in the Maltese Islands. A significant association was observed between these contaminants and upper and lower airway atopy. Specific school, classroom, cleaning and maintenance characteristics have been identified as having a direct impact on indoor air quality.

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“…The six fungal species were chosen because they are commonly found in homes and are known for their allergic and/or toxic effects (Chew et al, 2003;Huttunen et al, 2004;Kaarakainen et al, 2009;Kuhn and Ghannoum, 2003;Meklin et al, 2004). Mycobacteria and Streptomyces were selected for their allergic and inflammatory strength (Huttunen et al, 2004;Obihara et al, 2007).…”

Section: Introductionmentioning

confidence: 99%