Microbial Decontamination of Parathion and p-Nitrophenol in Aqueous Media

Munnecke, Douglas M.; Hsieh, Dennis P. H.

doi:10.1128/aem.28.2.212-217.1974

Cited by 114 publications

(42 citation statements)

References 1 publication

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“…Microbial activity has been deemed the most influential and significant cause of organophosphorus pesticide removal. Therefore, biodegradation is considered to be a reliable and cost-effective technique for pesticide abatement and a major factor determining the fate of organophosphorus pesticides in the environment [4]. There is an increasing need to develop new methods to detect, isolate and characterize the strains playing a part in these degradation processes [5].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium

Yang

Zhao

Zhang

et al. 2005

FEMS Microbiology Letters

191

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A bacterium, isolated from contaminated soils around a chemical factory and named strain DSP3 was capable of biodegrading both chlorpyrifos and 3,5,6-trichloro-2-pyridinol. Based on the results of phenotypic features, phylogenetic similarity of 16S rRNA gene sequences, DNA G+C content, and DNA homology between strain DSP3 and reference strains, strain DSP3 was identified as Alcaligenes faecalis. Chlorpyrifos was utilized as the sole source of carbon and phosphorus by strain DSP3. We examined the role of strain DSP3 in the degradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol under different culture conditions. Parathion and diazinon could also be degraded by strain DSP3 when provided as the sole sources of carbon and phosphorus. An addition of strain DSP3 (10(8)cells g(-1)) to soil with chlorpyrifos (100 mg kg(-1)) resulted in a higher degradation rate than the one obtained from non-inoculated soils. Different degradation rates of chlorpyrifos in six types of treated soils suggested that soils used for cabbage growing in combination with inoculation of strain DSP3 showed enhanced microbial degradation of chlorpyrifos.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium

Yang

Zhao

Zhang

et al. 2005

FEMS Microbiology Letters

191

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“…Microbial activity has been deemed to be the most influential and significant cause of organophosphorus pesticide removal. Therefore, biodegradation is a reliable and cost-effective technique for pesticide abatement, and a major factor determining the fate of organophosphorus pesticides in the environment (Kertesz et al, 1994;Munnecke & Hsieh, 1974). Monocrotophos is characterized by a P-O-C linkage and amide bond, and has been reported to be degraded as a sole carbon or phosphorus source in liquid media by Pseudomonas aeruginosa sp., Clavibacter michiganense ssp.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a denitrifying monocrotophos-degradingParacoccussp. M-1

Jia

Cui

et al. 2006

FEMS Microbiology Letters

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A bacterium strain, which is capable of degrading monocrotophos, was isolated from sludge collected from the bottom of a wastewater treatment system of a chemical factory, and named M-1. On the basis of the results of the cellular morphology, physiological and chemotaxonomic characteristics and phylogenetic similarity of 16S rDNA gene sequences, the strain was identified as a Paracoccus sp. The ability of the strain to mineralize monocrotophos was investigated under different culture conditions. Other organophosphorus insecticides and amide herbicides were also degraded by M-1. The key enzyme (s) involved in the initial biodegradation of monocrotophos in M-1 was shown to be a constitutively expressed cytosolic protein. The addition of M-1 (10(6) CFU g(-1)) to fluvo-aquic soil and a high-sand soil containing monocrotophos (50 mg kg(-1)) resulted in a higher degradation rate than that obtained from noninoculated soil. This microbial culture has great potential utility for the bioremediation of wastewater or soil contaminated with organophosphorus pesticides and amide herbicides.

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“…The anticholinesterase activity of OPs is lost when any of the phosphoester groups in the molecule is hydrolysed by a phosphotriesterase enzyme (Serdar and Gibson 1985;McDaniel et al 1988). Several bacteria have been isolated with this phosphotriesterase activity (Mulbry and Karns 1989;Sethunathan and Yoshida 1973;Munnecke and Hsieh 1974;Shelton and Somich 1988;Tchelet et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Development of a simple and sensitive fluorimetric method for isolation of coumaphos-hydrolysing bacteria

Harcourt

Horne

Sutherland

et al. 2002

Lett Appl Microbiol

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Aims: To develop a simple, rapid and sensitive fluorimetric assay to detect, isolate and characterize a soil bacterium capable of degrading the organophosphorus pesticide, coumaphos. Methods and Results: A high throughput microtitre plate-based method was used to quantify coumaphos hydrolysis by the bacterium. The fluorescent hydrolysis product of coumaphos, chlorferon, was detected at levels as low as 10 nmol l )1 . Incorporation of coumaphos into agar plates allowed the rapid detection of coumaphos-hydrolysing bacteria when exposed to an excitation wavelength of approximately 340 nm. The coumaphos-hydrolysing enzyme could be visualized when bacterial cell extracts were separated on SDS-PAGE, incubated with coumaphos and exposed to an excitation source as above. Conclusions:This method is 100-fold more sensitive than the currently used spectrophotometric method for coumaphos. Significance and Impact of the Study: This is a unique and versatile tool to screen for bacteria possessing phosphotriesterase activity.

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Microbial Decontamination of Parathion and p-Nitrophenol in Aqueous Media

Cited by 114 publications

References 1 publication

Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium

Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium

Isolation and characterization of a denitrifying monocrotophos-degradingParacoccussp. M-1

Development of a simple and sensitive fluorimetric method for isolation of coumaphos-hydrolysing bacteria

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