Microbial degradation of petroleum hydrocarbons

Varjani, Sunita

doi:10.1016/j.biortech.2016.10.037

Cited by 1,044 publications

(561 citation statements)

References 69 publications

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“…Therefore, less information is available about the genes and enzymes involved in these pathways. During degradation of PHs, anaerobic bacteria offer nutrients required for the growth of other catabolizing bacteria (Varjani, 2017). A large variety of microorganisms (bacteria and archaea) have been identified with the capability to degrade hydrocarbon molecules anaerobically.…”

Section: Anaerobic Biodegradationmentioning

confidence: 99%

Plant-bacteria synergism: An innovative approach for the remediation of crude oil-contaminated soils

Fatima¹,

Imran²,

Naveed³

et al. 2017

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Section: Anaerobic Biodegradationmentioning

confidence: 99%

Plant-bacteria synergism: An innovative approach for the remediation of crude oil-contaminated soils

Fatima¹,

Imran²,

Naveed³

et al. 2017

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Section: Introductionmentioning

confidence: 99%

“…The biodegradation of PCO by microorganisms has been the subject of many excellent reviews during the past decade (Hamamura et al, 2006; Kanaly and Harayama, 2010; Silva et al, 2014; Varjani, 2017). A considerable number of investigations has reported that bacteria are the most active microorganisms in crude oil degradation, and several bacteria are even known to feed exclusively on hydrocarbons (Acosta-Gonzalez and Marques, 2016; Varjani, 2017). Other organisms, including some archaea and a few yeast genera, namely, Candida, Yarrowia, Pichia , and more recently Saccharomyces are also described as powerful degraders of hydrocarbons and more particularly the ALKs (Iida et al, 2000; Hanano et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Biochemical, Molecular, and Transcriptional Highlights of the Biosynthesis of an Effective Biosurfactant Produced by Bacillus safensis PHA3, a Petroleum-Dwelling Bacteria

2017

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Petroleum crude oil (PCO)-dwelling microorganisms have exceptional biological capabilities to tolerate the toxicity of petroleum contaminants and are therefore promising emulsifier and/or degraders of PCO. This study describes a set of PCO-inhabiting bacterial species, one of which, identified as Bacillus safensis PHA3, produces an efficient biosurfactant which was characterized as a glycolipid. Fourier transform infrared spectrometer, nuclear magnetic resonance, Thin layer chromatography, HPLC, and GC-MS analysis of the purified biosurfactant revealed that the extracted molecule under investigation is likely a mannolipid molecule with a hydrophilic part as mannose and a hydrophobic part as hexadecanoic acid (C16:0). The data reveal that: (i) PHA3 is a potential producer of biosurfactant (9.8 ± 0.5 mg mL-1); (ii) pre-adding 0.15% of the purified glycolipid enhanced the degradation of PCO by approximately 2.5-fold; (iii) the highest emulsifying activity of biosurfactant was found against the PCO and the lowest was against the naphthalene; (iv) the optimal PCO-emulsifying activity was found at 30–60°C, pH 8 and a high salinity. An orthologous gene encodes a putative β-diglucosyldiacylglycerol synthase (β-DGS) was identified in PHA3 and its transcripts were significantly up-regulated by exogenous PAHs, i.e., pyrene and benzo(e)pyrene but much less by mid-chain n-alkanes (ALKs) and fatty acids. Subsequently, the accumulation of β-DGS transcripts coincided with an optimal growth of bacteria and a maximal accumulation of the biosurfactant. Of particular interest, we found that PHA3 actively catalyzed the degradation of PAHs notably the pyrene and benzo(e)pyrene but was much less effective in the mono-terminal oxidation of ALKs. Such characteristics make Bacillus safensis PHA3 a promising model for enhanced microbial oil recovery and environmental remediation.

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“…Many genes encoding proteins for degradation of hydrocarbons has been studied [1,8], yet the proteins involved in the alkane uptake have not been much worked out. The mechanism of alkanes enter the cell may differ depending on the bacterial species considered, the molecular weight of the alkane and the physico-chemical characteristics of the environment [9,10].…”

Section: Introductionmentioning

confidence: 99%

Quantitative proteomics analysis of proteins involved in alkane uptake comparing the profiling of Pseudomonas aeruginosa SJTD-1 in response to n-octadecane and n-hexadecane

et al. 2017

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While many data are available on genes encoding proteins for degradation of hydrocarbons in bacteria, the impact of alkane on transporter protein expression is unclear. Pseudomonas aeruginosa SJTD-1 is a strain that can consume medium- and long-chain n-alkanes. In order to study the proteins involved in n-octadecane uptake, we use iTRAQ and label free comparative proteomics analysis to identify the proteins of alkane uptake in response to n-octadecane (C18) comparing with n-hexadecane (C16) in P. aeruginosa SJTD-1. A total of 1102 and 1249 proteins were identified by iTRAQ-based and label free quantitative methodologies, respectively. By application of 1.5 (iTRAQ) or 2-fold (label free) for upregulated and 0.65 (iTRAQ) or 0.5-fold (label free) for downregulated cutoff values, 91 and 99 proteins were found to be differentially expressed comparing SJTD-1 cultivated on C18 with C16 respectively. There are six proteins with the common differential expression by iTRAQ and label free-based methods. Results of bioinformational analysis suggested the involvement of bacterial chemotaxis in responds to C18. Additionally, quantitative reverse transcriptase PCR (qRT-PCR) results confirmed C18-induced change in levels of FleQ, FliC, NirS, FadL and FadD proteins and the role of the proteins in n-octadecane uptake was further discussed in P. aeruginosa. In conclusion, results of the present study provided information about possible target-related proteins of bacterial chemotaxis, swimming performance, alkane transport to stimulus of n-ctadecane rather than n-hexadecane in P. aeruginosa SJTD-1.

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Microbial degradation of petroleum hydrocarbons

Cited by 1,044 publications

References 69 publications

Plant-bacteria synergism: An innovative approach for the remediation of crude oil-contaminated soils

Plant-bacteria synergism: An innovative approach for the remediation of crude oil-contaminated soils

Biochemical, Molecular, and Transcriptional Highlights of the Biosynthesis of an Effective Biosurfactant Produced by Bacillus safensis PHA3, a Petroleum-Dwelling Bacteria

Quantitative proteomics analysis of proteins involved in alkane uptake comparing the profiling of Pseudomonas aeruginosa SJTD-1 in response to n-octadecane and n-hexadecane

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