Microbial degradation of polycyclic aromatic hydrocarbons: effect of substrate availability on bacterial growth kinetics

Volkering, F.; Breure, A.M.; Sterkenburg, A.; Andel, J. van

doi:10.1007/bf00170201

Cited by 191 publications

(126 citation statements)

References 16 publications

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“…38 Other Model Approaches. The Best equation 15,39,15,18,19 balances diffusive flux to the bacterium with metabolic flux; both can limit substrate degradation. A shortcoming of the concept is that it requires steady-state, i.e., equal dissolution and metabolism kinetics constant over time.…”

Section: ■ Discussionmentioning

confidence: 99%

Experimental Results and Integrated Modeling of Bacterial Growth on an Insoluble Hydrophobic Substrate (Phenanthrene)

Adam

Rein

Miltner

et al. 2014

Environ. Sci. Technol.

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Metabolism of a low-solubility substrate is limited by dissolution and availability and can hardly be determined. We developed a numerical model for simultaneously calculating dissolution kinetics of such substrates and their metabolism and microbial growth (Monod kinetics with decay) and tested it with three aerobic phenanthrene (PHE) degraders: Novosphingobium pentaromativorans US6-1, Sphingomonas sp. EPA505, and Sphingobium yanoikuyae B1. PHE was present as microcrystals, providing non-limiting conditions for growth. Total PHE and protein concentration were tracked over 6–12 days. The model was fitted to the test results for the rates of dissolution, metabolism, and growth. The strains showed similar efficiency, with v max values of 12–18 g dw g–1 d–1, yields of 0.21 g g–1, maximum growth rates of 2.5–3.8 d–1, and decay rates of 0.04–0.05 d–1. Sensitivity analysis with the model shows that (i) retention in crystals or NAPLs or by sequestration competes with biodegradation, (ii) bacterial growth conditions (dissolution flux and resulting chemical activity of substrate) are more relevant for the final state of the system than the initial biomass, and (iii) the desorption flux regulates the turnover in the presence of solid-state, sequestered (aged), or NAPL substrate sources.

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Section: ■ Discussionmentioning

confidence: 99%

Experimental Results and Integrated Modeling of Bacterial Growth on an Insoluble Hydrophobic Substrate (Phenanthrene)

Adam

Rein

Miltner

et al. 2014

Environ. Sci. Technol.

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“…Various remediation, and in particular bioremediation procedures, are available (Alexander 1999;Wilson and Jones 1993). Since PAHs are hydrophobic compounds with low solubility in water, they have a great tendency to bind to organic matter or soil, limiting their availability to microorganisms (Volkering et al 1992;Pignatello and Xing 1996;Carmichael et al 1997;Zhang et al 1998). The addition of organic materials can enhance biodegradation by improving soil texture and oxygen transfer and by providing energy sources to rapidly establish a large microbial population (Englert et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Remediation of polycyclic aromatic hydrocarbon (PAH) contaminated soil through composting with fresh organic wastes

Zhang

Zhu

Houot

et al. 2011

Environ Sci Pollut Res

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Introduction Composting may enhance bioremediation of PAH-contaminated soils by providing organic substrates that stimulate the growth of potential microbial degraders. However, the influence of added organic matter (OM) together with the microbial activities on the dissipation of PAHs has not yet been fully assessed. Materials and methods An in-vessel composting-bioremediation experiment of a contaminated soil amended with fresh wastes was carried out. Four different experimental conditions were tested in triplicate during 60 days using laboratory-scale reactors: treatment S (100% soil), W (100% wastes), SW (soil/ waste mixture), and SWB (soil/waste mixture with inoculation of degrading microorganisms). Results and discussion A dry mass loss of 35±5% was observed in treatments with organic wastes during composting in all the treatments except treatment S. The dissipation of the 16 USEPA-listed PAHs was largely enhanced from no significant change to 50.5±14.8% (for SW)/63.7±10.0% (for SWB). More obvious dissipation was observed when fresh wastes were added at the beginning of composting to the contaminated soil, without significant difference between the inoculated and non-inoculated treatments. Phospholipid fatty acid (PLFA) profiling showed that fungi and G-bacteria dominated at the beginning of experiment and were probably involved in PAH dissipation. Subsequently, greater relative abundances of G+bacteria were observed as PAH dissipation slowed down. Conclusions The results suggest that improving the composting process with optimal organic compositions may be a feasible remediation strategy in PAH-contaminated soils through stimulation of active microbial populations.

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“…This tendency is increasing with the number of their aromatic rings (Kleineidam et al, 2002;Kubicki, 2006). Sorption and poor solubility in water makes the major fraction of PAHs inaccessible for bacterial degradation (Bosma et al, 1997;Volkering et al, 1992). Although one controversial study reported on bacteria that directly accessed the sorbed substrate (Guerin and Boyd, 1997), presently it is believed that PAHs adsorbed on soil particles, solid PAH crystals, or hydrocarbons dissolved in non-aqueous phase liquids (NAPLs) remain unavailable to bacteria (Johnsen et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Evidence for in situ degradation of mono-and polyaromatic hydrocarbons in alluvial sediments based on microcosm experiments with 13C-labeled contaminants

Morasch

Höhener

Hunkeler

2007

Environmental Pollution

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A method based on 13 C-labeled substrates was developed to determine the intrinsic biodegradation potential of aromatic pollutants under oxic and under anoxic conditions. AbstractA microcosm study was conducted to investigate the degradation of mono-and polyaromatic hydrocarbons under in situ-like conditions using alluvial sediments from the site of a former cokery. Benzene, naphthalene, or acenaphthene were added to the sediments as 13 C-labeled substrates. Based on the evolution of 13 C-CO 2 determined by gas chromatography isotope-ratio mass spectrometry (GC-IRMS) it was possible to prove mineralization of the compound of interest in the presence of other unknown organic substances of the sediment material. This new approach was suitable to give evidence for the intrinsic biodegradation of benzene, naphthalene, and acenaphthene under oxic and also under anoxic conditions, due to the high sensitivity and reproducibility of 13 C/ 12 C stable isotope analysis. This semi-quantitative method can be used to screen for biodegradation of any slowly degrading, strongly sorbing compound in long-term experiments.

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Microbial degradation of polycyclic aromatic hydrocarbons: effect of substrate availability on bacterial growth kinetics

Cited by 191 publications

References 16 publications

Experimental Results and Integrated Modeling of Bacterial Growth on an Insoluble Hydrophobic Substrate (Phenanthrene)

Experimental Results and Integrated Modeling of Bacterial Growth on an Insoluble Hydrophobic Substrate (Phenanthrene)

Remediation of polycyclic aromatic hydrocarbon (PAH) contaminated soil through composting with fresh organic wastes

Evidence for in situ degradation of mono-and polyaromatic hydrocarbons in alluvial sediments based on microcosm experiments with 13C-labeled contaminants

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