Microbial Degradation, Spectral analysis and Toxicological Assessment of Malachite Green Dye by Streptomyces exfoliatus

Abu-Hussien, Samah H.; Hemdan, Bahaa A.; Alzahrani, Othman M.; Alswat, Amal S.; Alatawi, Fuad A.; Alenezi, Muneefah Abdullah; Darwish, Doaa Bahaa Eldin; Bafhaid, Hanouf Saeed; Mahmoud, Samy F.; Ibrahim, Mohamed; El-Sayed, Salwa M.

doi:10.3390/molecules27196456

Cited by 24 publications

(18 citation statements)

References 43 publications

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“…Malachite green, a commonly used dye in the textile industry, is considered an environmental pollutant with adverse effects on all organisms [7]. Owing to its reported toxicity, it is essential to properly treat dye‐containing effluents before their discharge into the environment [4].…”

Section: Discussionmentioning

confidence: 99%

“…Malachite green is reported to be involved in mutagenesis, carcinogenesis, chromosomal fractures, and respiratory issues [4]. The toxicity of the malachite green depends on different factors including increase in concentration, exposure time, and temperature [7]. In view of the eco‐toxic attributes of malachite green, there is a dire need to develop protocols for effective treatment of malachite green‐containing effluent.…”

Section: Introductionmentioning

confidence: 99%

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Toward a zero waste approach: Utilization of sugarcane bagasse for dye removal and multienzymes production

Ejaz,

Taj,

Sohail

et al. 2023

J Basic Microbiol

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Global production of sugarcane bagasse (SB) by sugar industries exceeds more than 100 tons per annum. SB is rich in lignin and polysaccharide and hence can serve as a low‐cost energy and carbon source for the growth of industrially important microorganism. However, various other applications of SB have also been investigated. In this study, SB was used as an adsorbent to remove an azo dye, malachite green. Subsequently, the dye‐adsorbed SB was fermented by Trametes pubescens MB 89 for the production of laccase enzyme. The fungal pretreated SB was further utilized as a substrate for the simultaneous production of multiple plant cell wall degrading enzymes including, cellulase, xylanase, pectinase, and amylase by thermophilic bacterial strains. Results showed that 0.1% SB removed 97.04% malachite green at 30°C after 30 min from a solution containing 66 ppm of the dye. Fermentation of the dye‐adsorbed SB by T. pubescens MB 89 yielded 667.203 IU mL−1 laccase. Moreover, Brevibacillus borstelensis UE10 produced 38.41 and 18.6 IU mL−1 β‐glucosidase and pectinase, respectively, by using fungal‐pretreated SB. Cultivation of B. borstelensis UE27 in the medium containing the same substrate yielded 32.14 IU mL−1 of endoglucanase and 27.23 IU mL−1 of β‐glucosidase. Likewise, Neobacillus sedimentimangrovi UE25 could produce a mix of β‐glucosidase (37.24 IU mL−1), xylanase (18.65 IU mL−1) and endoglucanase (26.65 IU mL−1). Hence, this study led to the development of a method through which dye‐containing textile effluent can be treated by SB along with the production of industrially important enzymes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Toward a zero waste approach: Utilization of sugarcane bagasse for dye removal and multienzymes production

Ejaz,

Taj,

Sohail

et al. 2023

J Basic Microbiol

View full text Add to dashboard Cite

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“…The formazan crystals formed by the live cells were then dissolved with 100µL of absolute dimethyl sulfoxide (DMSO). The absorbance of formazan solutions was measured at λmax 570nm using a multi-well plate reader 23 .…”

Section: Cytotoxicity Assay Of Agnps From a Bisporusmentioning

confidence: 99%

Biosynthesis, characterization of silver nano particles from Agaricus bisporus (Mushroom) and their antimicrobial activity against foodborne bacterial pathogens

Amr,

Abd-Elhalim,

Ismail

et al. 2023

Preprint

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Biosynthesized silver nanoparticles (AgNPs) attracted broad interest in the food sector due to their excellent antimicrobial activities as a new alternative to harmful chemical disinfectants. This work aims to study characterization parameters, antimicrobial and toxicological characteristics of the biosynthesized silver nanoparticles from Agaricus bisporus (Mushroom) aqueous extract. Mushroom extract was treated with silver nitrate and incubated for 24h until its color was turned brown. AgNPs characterization studies were estimated for their stability, their characteristics using UV and visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HR-TEM), Dynamic light scattering (DLS) and energy dispersive X-ray spectroscopy (XRD). All synthesized particles had the maximum absorption at 456nm at 1.5 Surface plasmon resonance (SPR), indicating the biosynthesis of nano silver particles. In addition. AgNPs from A. bisporus (Mushroom) exhibited broad antibacterial action against eight foodborne bacterial strains. Among all the tested bacterial strains, P. aeruginosa ATCC 27853 was the superlative sensitive strain with inhibition zone of 48mm, while IZD for K. pneumonia ATCC 700603 and B. cereus ATCC 11778 were the most resistant strains with IZD of 20mm. According to the MIC/MBC ratio, the silver cations released by AgNPs had strong bactericidal effects against both G+ ve and G− ve bacteria. Cytotoxicity test against normal human skin fibroblast indicated that silver nano silver particles from the mushroom extract were safe, exhibiting cell viability reached 98.2%. Therefore, AgNPs could be a good alternative source to inhibit the biofilm formation in the food industry sector.

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“…Ten concentrations of twofold serially diluted MOL were prepared. The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) assay was conducted in reference to 13 , where con uent monolayers of OEC were cultured for 24 h in a 96 well-microtiter plate. The cells were cultured in triplicates with varying doses of the tested MOL at 37°C for 72 h under a CO 2 environment.…”

Section: Bio Lm Inhibition Of Pathogenic Bacteria Strainsmentioning

confidence: 99%

“…It was done according to the assay of Abu-Hussien, et al 2022. 13 2.9. Statistical analysis All data were analyzed using IBM SPSS package.…”

Section: Gas Chromatography (Gc) For Cockroach Hemolymphmentioning

confidence: 99%

Antimicrobial peptides from Periplaneta americana cockroaches inhibit pathogenic bacterial biofilm formation

Abu-Hussein

Adly

Radwan

et al. 2023

Preprint

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The food industry sector faces many obstacles all over the world due to biofilm formation causing economical huge losses and serious problems to human health. The present work was designed to discover new natural alternatives of effective antimicrobial peptides from insects against three pathogenic bacterial strains Escherichia coli O157:H7, Bacillus cereus ATCC11778 and Staphylococcus aureus ATCC29737. A total of 150 adult P. americana were reared and hemolymph was extracted to test their inhibitory activities. The adult P. americana hemolymph extract markedly inhibited the growth of B. cereus ATCC11778 and S. aureus ATCC2973 while E. coli O157:H7 was resistant. All cockroaches insects' hemolymph extraction and different concentrations of hemolymph were prepared then tested against the previously mentioned bacterial pathogens applying the Clinical Laboratory Standard Institute (CLSI) methods. Minimum inhibitory concentration of the extracts was also determined with zones of inhibition ranging from 10.0 to 30.0 mm. The highest value of the inhibition zone was for B. cereus, which recorded 30.0 mm for both crude extract and 0.5 mg/ml of hemolymph extraction. Crystal violet staining experiment was carried for testing biofilm inhibition. As a result, the formed biofilm of B. cereus and S. aureus was strongly inhibited by hemolymph and destroyed after 48 hrs of incubation with optical density of 0.388 and 0.0503 compared to control treatments of OD readings reached 1.91, at 590 nm, respectively. The cytotoxicity test against human skin fibroblast indicated no toxicity effect of the hemolymph. The Gas chromatography analysis of the adult Periplaneta americana cockroach hemolymph indicated the presence of antimicrobial peptides and fatty acids. The predominant antimicrobial peptides were 3,5-Triazine, 2,4-Diamine, 6-Chloro-N-Ethyl, N,N,N'-Trimethyl-N'-(4-Meth Oxy-Cis-Cinnamoyl-Putresc, 3',8,8'-Trimethoxy-3-piperidyl-2,2'-b inaphthalene-1,1',4,4'-tetrone at retention time (RT) of 6.99, 27.67 and 36.61 min, respectively. The most abundant Fatty acids were hexadecanoic acid, dodecanoic acid, Octadecanedioic acid at RT of 4.15 min. The inhibitory potency of hemolymph extract from Periplaneta americana as a new insectary alternative against microbial growth and biofilm formation makes this insect a new promising source for effective production of antimicrobial peptides (AMPs).

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Microbial Degradation, Spectral analysis and Toxicological Assessment of Malachite Green Dye by Streptomyces exfoliatus

Cited by 24 publications

References 43 publications

Toward a zero waste approach: Utilization of sugarcane bagasse for dye removal and multienzymes production

Toward a zero waste approach: Utilization of sugarcane bagasse for dye removal and multienzymes production

Biosynthesis, characterization of silver nano particles from Agaricus bisporus (Mushroom) and their antimicrobial activity against foodborne bacterial pathogens

Antimicrobial peptides from Periplaneta americana cockroaches inhibit pathogenic bacterial biofilm formation

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