Microbial Diversity and Abundance in a Representative Small-Production Coal Mine of Central China

Wei, Min; Yu, Zhisheng; Zhang, Hongxun

doi:10.1021/ef400529f

Cited by 33 publications

(17 citation statements)

References 75 publications

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“…In particular, the genus Pseudomonas , which contains facultative members, has been found in several other coal microbial communities surveys, including in the Sydney Basin (Li et al ., ), in two Chinese coal seam studies (Tang et al ., ; Wei et al ., ) and in the western Canadian Sedimentary basin (Penner et al ., ; An et al ., ) and Pseudomonas stutzeri has been found in Indian coal (Singh et al ., ). In addition, as it was not possible to break‐down coal without a simpler carbon co‐substrate, a pure culture of P. stutzeri was found to increase the production of biosurfactant in the rhamnolipid class when grown with coal and other hydrocarbon products (Singh & Tripathi, ).…”

Section: Discussionmentioning

confidence: 99%

The influence of hydrogeological disturbance and mining on coal seam microbial communities

et al. 2015

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The microbial communities present in two underground coal mines in the Bowen Basin, Queensland, Australia, were investigated to deduce the effect of pumping and mining on subsurface methanogens and methanotrophs. The micro-organisms in pumped water from the actively mined areas, as well as, pre- and post-mining formation waters were analyzed using 16S rRNA gene amplicon sequencing. The methane stable isotope composition of Bowen Basin coal seam indicates that methanogenesis has occurred in the geological past. More recently at the mine site, changing groundwater flow dynamics and the introduction of oxygen in the subsurface has increased microbial biomass and diversity. Consistent with microbial communities found in other coal seam environments, pumped coal mine waters from the subsurface were dominated by bacteria belonging to the genera Pseudomonas and the family Rhodocyclaceae. These environments and bacterial communities supported a methanogen population, including Methanobacteriaceae, Methanococcaceae and Methanosaeta. However, one of the most ubiquitous micro-organisms in anoxic coal mine waters belonged to the family 'Candidatus Methanoperedenaceae'. As the Archaeal family 'Candidatus Methanoperedenaceae' has not been extensively defined, the one studied species in the family is capable of anaerobic methane oxidation coupled to nitrate reduction. This introduces the possibility that a methane cycle between archaeal methanogenesis and methanotrophy may exist in the anoxic waters of the coal seam after hydrogeological disturbance.

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Section: Discussionmentioning

confidence: 99%

The influence of hydrogeological disturbance and mining on coal seam microbial communities

et al. 2015

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“…Another genus of acetoclastic methanogens, Methanosarcina, is often abundant in methanogenic soils and sediments (23)(24)(25), coal mines (26,27), landfills (28), and anaerobic digesters (29,30). Several studies suggested that Methanosarcina species could accept electrons from nonbiological extracellular surfaces (31,32).…”

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Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri

Rotaru

Shrestha

Liu

et al. 2014

Appl Environ Microbiol

784

518

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c Direct interspecies electron transfer (DIET) is potentially an effective form of syntrophy in methanogenic communities, but little is known about the diversity of methanogens capable of DIET. The ability of Methanosarcina barkeri to participate in DIET was evaluated in coculture with Geobacter metallireducens. Cocultures formed aggregates that shared electrons via DIET during the stoichiometric conversion of ethanol to methane. Cocultures could not be initiated with a pilin-deficient G. metallireducens strain, suggesting that long-range electron transfer along pili was important for DIET. Amendments of granular activated carbon permitted the pilin-deficient G. metallireducens isolates to share electrons with M. barkeri, demonstrating that this conductive material could substitute for pili in promoting DIET. When M. barkeri was grown in coculture with the H 2 -producing Pelobacter carbinolicus, incapable of DIET, M. barkeri utilized H 2 as an electron donor but metabolized little of the acetate that P. carbinolicus produced. This suggested that H 2 , but not electrons derived from DIET, inhibited acetate metabolism. P. carbinolicus-M. barkeri cocultures did not aggregate, demonstrating that, unlike DIET, close physical contact was not necessary for interspecies H 2 transfer. M. barkeri is the second methanogen found to accept electrons via DIET and the first methanogen known to be capable of using either H 2 or electrons derived from DIET for CO 2 reduction. Furthermore, M. barkeri is genetically tractable, making it a model organism for elucidating mechanisms by which methanogens make biological electrical connections with other cells.

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“…When compared to previous studies of coal mines in Hubei (Wei et al, 2013), the sampling mine in the present study is a representative gassy coal mine. The depth of the samples was 158 ± 5 m, and the average thickness of the coal seam was 0.8 m. The methane concentration in ventilation air varies from 0.3% to 1.7% over a year.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine

Wei

Jiang

et al. 2014

International Journal of Coal Geology

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Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine, International Journal of Coal Geology (2014), doi: 10.1016/j.coal.2014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT / 57Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine yuzs@ucas.ac.cn AbstractThe microbial communities and biogenic methane potential of a gas coal mine were investigated by cultivation-independent and cultivation-dependent approaches. Stable carbon isotopic analysis indicated that in situ methane in the coal mine was dominantly of a thermogenic origin. However, a high level of diversity of bacteria and methanogens that were present in the coal mine was revealed by 454 pyrosequencing, and included various fermentative bacteria in the phyla of Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria, and acetotrophic, hydrogenotrophic, and methylotrophic methanogens.Methane was produced in enrichments of mine water samples supplemented with acetate under laboratory conditions. The microbial flora obtained from the enrichments could stimulate methane formation from coal samples. 16S rRNA gene clone library analysisindicated that the microbial community from coal cultivation samples supplemented with the enriched microbial consortium was dominated by the anaerobic fermentative Clostridiales and facultative acetoclastic Methanosarcina. This study suggests that the biogenic methane potential in the thermogenic-gas coal mine could be stimulated by the indigenous microorganisms.

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Microbial Diversity and Abundance in a Representative Small-Production Coal Mine of Central China

Cited by 33 publications

References 75 publications

The influence of hydrogeological disturbance and mining on coal seam microbial communities

The influence of hydrogeological disturbance and mining on coal seam microbial communities

Direct Interspecies Electron Transfer between Geobacter metallireducens and Methanosarcina barkeri

Microbial diversity and biogenic methane potential of a thermogenic-gas coal mine

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