Microbial Diversity and Community Assembly across Environmental Gradients in Acid Mine Drainage

Teng, Wenkai; Kuang, Jialiang; Luo, Zhen-Hao; Shu, Wen‐Sheng

doi:10.3390/min7060106

Cited by 41 publications

(13 citation statements)

References 73 publications

(116 reference statements)

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“…The high abundance of halophilic bacteria in P1 and P2 (e.g., Fodinibius , Alkalilimnicola , Phycisphaera , and Gp21) were also common in marine and sediments (Fukunaga et al, 2009; Sorokin et al, 2010; Wang et al, 2012). Acidobacteria were reported frequently as dominant members in some mining environments (Teng et al, 2017). Its numerical dominance in P1 and P2 thus seemed to indicate its (potentially) important role in sulfur and iron cycling in these tailings.…”

Section: Discussionmentioning

confidence: 99%

Variations in Soil Bacterial Composition and Diversity in Newly Formed Coastal Wetlands

Wen-bing

Ruan

et al. 2019

Front. Microbiol.

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Coastal ecosystems experience some of the most active land–ocean interactions in the world, and they are characterized by high primary productivity and biological diversity in the sediment. Given the roles of microorganisms in soil biogeochemical cycling and their multifaceted influence on soil ecosystems, it is critical to understand the variations and drivers of soil microbial communities across coastal ecosystems. Here, we studied soil bacterial community dynamics at different sites (from seawater to freshwater) in the Yellow River Delta, China. Bacterial community composition and diversity over four seasons were analyzed through 16S rRNA genes. Notably, the bacterial community near the ocean had the lowest alpha-diversity when compared with the other sites. No significant differences in bacterial communities among seasons were found, indicating that seasonal variation in temperature had little influence on bacterial community in the newly formed wetlands in the Yellow River Delta. Bacterial community structure changed substantially along the salinity gradient, revealing a clear ecological replacement along the gradual transformation gradient from freshwater to seawater environment. Redundancy analysis revealed that salinity was the main driver of variations in bacterial community structure and explained 17.5% of the variability. Our study provides a better understanding of spatiotemporally determined bacterial community dynamics in coastal ecosystems.

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Section: Discussionmentioning

confidence: 99%

Variations in Soil Bacterial Composition and Diversity in Newly Formed Coastal Wetlands

Wen-bing

Ruan

et al. 2019

Front. Microbiol.

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“…The most dominant bacterial populations residing in AMD are highly acidophilic, chemolithoautotrophic iron and sulfur oxidizers such as Acidithiobacillus , Leptospirillum , Ferrithrix , and Ferritrophicum etc. (Baker and Banfield, 2003; Chen et al, 2015, 2016; Méndez-García et al, 2015; Huang et al, 2016; Mesa et al, 2017; Teng et al, 2017). These acidophilic, autotrophic and Fe/S oxidizing microorganisms mainly contribute toward AMD generation and were studied extensively for their physiology, molecular mechanisms and ecological relevance (Denef et al, 2010; Kuang et al, 2013; Méndez-García et al, 2014; Chen et al, 2015; Goltsman et al, 2015; Chen et al, 2016), whereas the small heterotrophic populations thriving in the same niches could be of great significance in reducing AMD generation process and attenuating the overall hazard of these systems remain less explored.…”

Section: Introductionmentioning

confidence: 99%

Low-Abundance Members of the Firmicutes Facilitate Bioremediation of Soil Impacted by Highly Acidic Mine Drainage From the Malanjkhand Copper Project, India

et al. 2018

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Sulfate- and iron-reducing heterotrophic bacteria represented minor proportion of the indigenous microbial community of highly acidic, oligotrophic acid mine drainage (AMD), but they can be successfully stimulated for in situ bioremediation of an AMD impacted soil (AIS). These anaerobic microorganisms although played central role in sulfate- and metal-removal, they remained inactive in the AIS due to the paucity of organic carbon and extreme acidity of the local environment. The present study investigated the scope for increasing the abundance and activity of inhabitant sulfate- and iron-reducing bacterial populations of an AIS from Malanjkhand Copper Project. An AIS of pH 3.5, high soluble SO42− (7838 mg/l) and Fe (179 mg/l) content was amended with nutrients (cysteine and lactate). Thorough geochemical analysis, 16S rRNA gene amplicon sequencing and qPCR highlighted the intrinsic metabolic abilities of native bacteria in AMD bioremediation. Following 180 days incubation, the nutrient amended AIS showed marked increase in pH (to 6.6) and reduction in soluble -SO42− (95%), -Fe (50%) and other heavy metals. Concomitant to physicochemical changes a vivid shift in microbial community composition was observed. Members of the Firmicutes present as a minor group (1.5% of total community) in AIS emerged as the single most abundant taxon (∼56%) following nutrient amendments. Organisms affiliated to Clostridiaceae, Peptococcaceae, Veillonellaceae, Christensenellaceae, Lachnospiraceae, Bacillaceae, etc. known for their fermentative, iron and sulfate reducing abilities were prevailed in the amended samples. qPCR data corroborated with this change and further revealed an increase in abundance of dissimilatory sulfite reductase gene (dsrB) and specific bacterial taxa. Involvement of these enhanced populations in reductive processes was validated by further enrichments and growth in sulfate- and iron-reducing media. Amplicon sequencing of these enrichments confirmed growth of Firmicutes members and proved their sulfate- and iron-reduction abilities. This study provided a better insight on ecological perspective of Firmicutes members within the AMD impacted sites, particularly their involvement in sulfate- and iron-reduction processes, in situ pH management and bioremediation.

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“…Within the domain of Archaea, Ferroplasma spp. have been the most frequently detected [8][9][10][11][12]. However, the acidophilic communities involved in AMD generation are very complex and include several microbial species; their composition and dynamics under different conditions have been widely documented [13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Influence of Extremophiles on the Generation of Acid Mine Drainage at the Abandoned Pan de Azúcar Mine (Argentina)

et al. 2021

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The risk of generation of acid drainages in the tailings of the Pan de Azúcar mine that closed its activities more than three decades ago, was evaluated through biooxidation studies using iron- and sulfur-oxidizing extremophilic leaching consortia. Most of tailings showed a high potential for generating acid drainage, in agreement with the results from net acid generation (NAG) assays. In addition, molecular analysis of the microbial consortia obtained by enrichment of the samples, demonstrated that native leaching microorganisms are ubiquitous in the area and they seemed to be more efficient in the biooxidation of the tailings than the collection microorganisms. The acid drainages detected at the site and those formed by oxidation of the tailings, produced a significant ecotoxicological effect demonstrated by a bioassay. These drainages, even at high dilutions, could seriously affect a nearby Ramsar site (Laguna de Pozuelos) that is connected to the Pan de Azúcar mine through a hydrological route (Cincel River).

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Microbial Diversity and Community Assembly across Environmental Gradients in Acid Mine Drainage

Cited by 41 publications

References 73 publications

Variations in Soil Bacterial Composition and Diversity in Newly Formed Coastal Wetlands

Variations in Soil Bacterial Composition and Diversity in Newly Formed Coastal Wetlands

Low-Abundance Members of the Firmicutes Facilitate Bioremediation of Soil Impacted by Highly Acidic Mine Drainage From the Malanjkhand Copper Project, India

Influence of Extremophiles on the Generation of Acid Mine Drainage at the Abandoned Pan de Azúcar Mine (Argentina)

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