Microbial diversity formed and maintained through substrate feedback regulation and delayed responses induced by Low-Dose Ionizing Radiation

Yang, Xinbin; Li, Shuaishuai; Song, Ganyu; Xu, Xinming; Hu, Dawei

doi:10.1016/j.actaastro.2021.07.027

Cited by 7 publications

(14 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These three species have been applied in many studies as typical microbes. [4,[24][25][26][27] Despite these three species are hard to be differentiated in the mixed culture under microscopy…”

Section: Strains and Culturesmentioning

confidence: 99%

Combination of direct boiling and glass beads increases the purity and accuracy of bacterial DNA extraction

Liu

et al. 2023

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Extraction of DNA is a key step in molecular biology experiments and important for counting tiny microbial individuals. Direct boiling and mechanical cell lysis like glass beads are two independent physical extraction methods, thus crossing the barriers of thresholds of magnitude in popular reagent kits or traditional spread plate method when non‐equilibrium phenomenon is ongoing. The two approaches above were combined to generate a new one. In three typical microbial species, direct boiling with glass beads significantly increased the purity of DNA solution compared with some other methods (p < 0.05). The qPCR results of them were closer to direct microscopy counting than some other methods. Therefore, it provides a new choice in extracting bacterial DNA for specific circumstances.

show abstract

Section: Strains and Culturesmentioning

confidence: 99%

Combination of direct boiling and glass beads increases the purity and accuracy of bacterial DNA extraction

Liu

et al. 2023

Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent researches have aimed at resolving this separation between theoretical prediction and experimental observation. For example, induced by LDXR, the dynamic mechanisms of the substrate feedback regulation and delayed responses were produced to cause asynchronous convergent uctuations of microbial populations, which can remarkably alleviate interspeci c competition, leading to the formation and maintenance of higher microbial diversity in OE (Yang et al 2021). The LDXR as an adverse factor can generate intermediate disturbance to cause stronger microbial stochastic growth rate, which determines the microbial population size directly, and inevitably gives rise to stronger random variations of the intra-and interspeci c competition process, the substrates production and consumption process.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, in-depth investigations should be carried out to reveal the information sharing mechanisms in OE with LDXR from biochemical and genetic levels in future researches. Furthermore, the speci c drugs might be developed to block information substances for effective prevention and control of microbial pollution, and the ecological effect of LDXR on bacterial communities succession in OE can also be applied for ecological engineering purposes, such as LDXR might be used to induce bacterial diversity for ecological restoration(Lim et al 2016) and arti cial ecosystem construction(Yang et al 2021).…”

mentioning

confidence: 99%

Information sharing promotes bacterial diversity in oligotrophic environment with low-dose X-ray radiation based on CA modeling and simulation

Zhang¹,

Li²

2023

Preprint

View full text Add to dashboard Cite

It remains unclear why bacterial diversity was formed and maintained in oligotrophic environment (OE) with low-dose X-ray radiation (LDXR) due to violation of the competitive exclusion principle in ecology. Based on microbial ecology, bioinformatics, cybernetics, experimental phenomena and data, a new hypothesis was proposed to elucidate information sharing mechanisms causingformation and maintenance of bacterial diversity in OE with LDXR. According to hypothesis, a valid cellular automata (CA) model was developed, the CA simulation sufficiently proved that as the bacterial individuals conduct cooperation to share information of substrate positions with each other selflessly, it can effectively increase the survival probabilities of all bacterial species, alleviate interspecific competition, and prevent any bacterial species from being dominant to be beneficial to the coexistence of most species, promoting bacterial diversity during spatiotemporal community succession in OE with LDXR. The results of CA simulation sufficiently confirm the proposed hypothesis andare highly similar to the observed phenomena and data in the experiments. This study could not only lay the theoretical foundation for understanding of the key role of information sharing plays in formation and maintenance of bacterial diversity but also offer effective guidance for bacterial prevention and control as well as other ecological engineering applications.

show abstract

“…e periodic LDUVR can be viewed as an unfavorable factor with intermediate disturbance, causing microbial stochastic growth response (SGR) along with random variation of the crucial processes tightly correlated with SGR, such as intra-and inter-specific competition [8,9]. is new dynamic mechanism is based on existing ecological investigations and drives microbial community succession with higher species diversity in artificial intelligence device with periodic LDUVR.…”

Section: Introductionmentioning

confidence: 99%

“…To describe the dynamic mechanisms causing microbial community successions in artificial intelligence devices with periodic LDUVR, highly valid kinetic models expressed by differential equations with kinetic parameters obeying different normal distributions were established based on the proposed assumptions, experimental phenomena, and data [9]. Additionally, a large number of microbial species and substrates were computer-generated.…”

Section: Introductionmentioning

confidence: 99%

Formation Mechanism of Microbial Diversity in Artificial Intelligence Devices due to Intermediate Disturbance by Low-Dose UV Radiation for Complementary Medicine

Yang

Wang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

The development of artificial intelligence devices in the complementary medicine field is rapid and the surface microbial diversity pollution was found with periodic low-dose ultraviolet radiation (LDUVR). Since artificial intelligence devices do not have enough different types of substrates for microbial communities, it is unclear how the great microbial diversity can emerge and persist, as this clearly defies the competitive exclusion principle of ecology. In this study, the 5 most common genera in the artificial intelligence devices, Escherichia, Pseudomonas, Streptococcus, Staphylococcus and Aeromonas have been sampled without and with periodic LDUVR, respectively. A new hypothesis was put up to clarify the construction and maintenance process of high microbiological diversity in artificial intelligence devices by comparing and evaluating the variations between the dynamic response characteristics of their relative abundances in the two scenarios as follows: the periodic LDUVR can be regarded as an adverse factor with intermediate disturbance, causing stronger microbial stochastic growth responses (SGR) which would inevitably give rise to stronger random variation of the other important processes tightly correlated with SGR, such as intra- and interspecific competition process, and substrates production and consumption process, which could effectively diminish the auto- and cross-correlation of stochastic processes of microbial populations, alleviating the intra- and inter-specific competitions. In artificial intelligence devices with LDUVR, these crucial succession processes can propel the microbial communities to generate and sustain a high species diversity. Finally, thorough Monte Carlo simulations were used to thoroughly confirm the idea. This research can build the theoretical groundwork, offer fresh viewpoints, and suggest potential microbial prevention strategies for the succession of microbial communities in LDUVR.

show abstract

Microbial diversity formed and maintained through substrate feedback regulation and delayed responses induced by Low-Dose Ionizing Radiation

Cited by 7 publications

References 39 publications

Combination of direct boiling and glass beads increases the purity and accuracy of bacterial DNA extraction

Combination of direct boiling and glass beads increases the purity and accuracy of bacterial DNA extraction

Information sharing promotes bacterial diversity in oligotrophic environment with low-dose X-ray radiation based on CA modeling and simulation

Formation Mechanism of Microbial Diversity in Artificial Intelligence Devices due to Intermediate Disturbance by Low-Dose UV Radiation for Complementary Medicine

Contact Info

Product

Resources

About