Microbial Diversity in the Edible Gall on White Bamboo Formed by the Interaction between Ustilago esculenta and Zizania latifolia

Tu, Zhihao; Yamada, Shotaro; Hu, Dagula; Ito, Yoshitada; Iwasaki, Tomohito; Yamaguchi, Akihiro

doi:10.1007/s00284-019-01693-w

“…Fungal species not listed in the MALDI-TOF/MS Biotyper database or not providing enough protein signals were submitted to the following DNA sequence analysis with a slight modi cation of an original protocol (Tu et al 2019).…”

Section: Identi Cation Of Fungal Species By Dna Sequence Analysismentioning

confidence: 99%

“…PCR ampli cation for ARISA: The PCR conditions were the same as those described in the sequencing analysis except that the concentration of each primer was 25 µmol/L and the number of PCR cycles was 35. The nal DNA solutions were submitted to PCR ampli cation using a universal HEX uorescencelabeled primer pair for the fungal ITS regions: HEX-Fun-1 (2234C 5'-GTTTCCGTAGGTGAACCTGC/3126T 5'-ATATGCTTAAGTTCAGCGGGT) and HEX-Fun-2 (1406F 5'-TGYACACACCGCCGT/3126T HEX 5'-ATATGCTTAAGTTCAGCGGGT) (Tu et al 2019). Ten microliters of the PCR product was submitted to fragment analysis (Fasmac).…”

Section: Pcr Ampli Cationmentioning

confidence: 99%

“…The DNA solution was used as a template for PCR ampli cation of the internal transcribed spacer (ITS) or D1/D2 regions of rDNA using primer pairs Fun-3 (ITS1F: 5'-GTAACAAGGT(T/C)TCCGT/ITS1R: 5'-CGTTCTTCATCGATG) or Fun-5 (NL1: 5'-GCATATCAATAAGCGGAGGAAAAG/NL4: 5'-GGTCCGTGTTTCAAGACGG) (Tu et al 2019). The PCR mixture contained 14.1 μL of injectable water, 2 μL of 10×Ex Taq buffer, 1.6 μL of 2.5 mmol/L dNTPs, 0.1 μL of Ex Taq (Takara Bio, Kusatsu, Japan), 0.2 μL of the primer mix (each in 50 μmol/L forward and reverse primers) (Fasmac, Atsugi, Japan) and 2 μL of DNA template.…”

Section: Pcr Ampli Cationmentioning

confidence: 99%

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Antifungal activities of vineyard-habitat wild yeast for grape gray-mold disease and its initial promotion of alcohol fermentation in spontaneous wine brewing

Yamaguchi

¹

,

Hudagula

²

,

Maeno

³

et al. 2022

Preprint

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Microorganisms, including native yeasts, are abundant in vineyard fields. Herein, we studied the possibility of using vineyard-derived wild yeast as a microbial pesticide against Botrytis cinerea, a pathogen that causes grape gray mold disease, to boost the initial alcohol production of spontaneously fermented wine. We identified the Saccharomyces cerevisiae strain KONDO170908, which showed the most effective antifungal activity in an ex vivo yeast dripping experiment on grape berries. This strain was utilized in an in vivo spray test on grape bunches in vineyard fields and was proven to significantly suppress gray mold disease on the grape berries in test plot #16 when the yeast was sprayed during both the flowering and ripening periods (morbidity 11.2% against 15.3% of the control plot, χ2 test, P < 0.0001). However, in test plot #17, spraying the yeast during only the ripening period had no effect (morbidity 16.3%). The grapes from each test plot were also submitted for spontaneous wine fermentation. Alcoholic fermentation of the grapes from test plot #16 provided the most active bubbling of CO2 gas and the highest ethanol production and colony counts over seven days of fermentation. Unique changes in the different strains of S. cerevisiae among the plots were observed throughout the early fermentation stage. Thus, yeast spraying during the flowering period might trigger modification of the entire microbiota and could ultimately contribute to promoting alcohol production in the spontaneously fermented wine.

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“…PCR ampli cation: The DNA solution was used as a template for PCR ampli cation of the internal transcribed spacer (ITS) or D1/D2 regions of rDNA using primer pairs Fun-3 (ITS1F: 5'-GTAACAAGGT(T/C)TCCGT/ITS1R: 5'-CGTTCTTCATCGATG) or Fun-5 (NL1: 5'-GCATATCAATAAGCGGAGGAAAAG/NL4: 5'-GGTCCGTGTTTCAAGACGG) (Tu et al 2019). The PCR mixture contained 14.1 µL of injectable water, 2 µL of 10×Ex Taq buffer, 1.6 µL of 2.5 mmol/L dNTPs, 0.1 µL of Ex Taq (Takara Bio, Kusatsu, Japan), 0.2 µL of the primer mix (each in 50 µmol/L forward and reverse primers) (Fasmac, Atsugi, Japan) and 2 µL of DNA template.…”

Section: Dna Extractionmentioning

confidence: 99%

Antifungal activities of vineyard-habitat wild yeast for grape gray-mold disease and its initial promotion of alcohol fermentation in spontaneous winemaking

Hudagula

¹

,

Maeno

²

,

Minami

³

et al. 2022

Preprint

Self Cite

0

View full text Add to dashboard Cite

Microorganisms, including native yeasts, are abundant in vineyard fields. Herein, we studied the possibility of using vineyard-derived wild yeast as a microbial pesticide against Botrytis cinerea, a pathogen that causes grape gray mold disease, to boost the initial alcohol production of spontaneously fermented wine. We identified the Saccharomyces cerevisiae strain KONDO170908, which showed the most effective antifungal activity in an ex vivo yeast dripping experiment on grape berries. This strain was utilized in an in vivo spray test on grape bunches in vineyard fields and was proven to significantly suppress gray mold disease on the grape berries in test plot #16 when the yeast was sprayed during both the flowering and ripening periods (morbidity 11.2% against 15.3% of the control plot, χ2 test, P < 0.0001). However, in test plot #17, spraying the yeast during only the ripening period had no effect (morbidity 16.3%). The grapes from each test plot were also submitted for spontaneous wine fermentation. Alcoholic fermentation of the grapes from test plot #16 provided the most active bubbling of CO2 gas and the highest ethanol production and colony counts over seven days of fermentation. Unique changes in the different strains of S. cerevisiae among the plots were observed throughout the early fermentation stage. Thus, yeast spraying during the flowering period might trigger modification of the entire microbiota and could ultimately contribute to promoting alcohol production in the spontaneously fermented wine.

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“…Grey Jiaobai and male Jiaobai have no economic value in cultivation, and are removed manually (Zhang et al, 2021). Research shows that population diversity or physiological race differentiation of U. esculenta may exist among Z. latifolia plants (Tu et al, 2019). The molecular mechanism underpinning the male Z. latifolia responses to U. esculenta infection remains unknown, although the mechanism of swelling and stem enlargement in infected Z. latifolia has been a focus of attention (Li et al, 2021(Li et al, , 2022Zhang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

MAP kinase and plant–pathogen interactions govern male Zizania latifolia responses to Ustilago esculenta during the early stages of infection

Guo

¹

,

Zhou

²

,

Bai

³

et al. 2023

Folia Horticulturae

3

0

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This study explored the effect of Ustilago esculenta infection on differentially expressed genes (DEGs) in male Manchurian wild rice (Zizania latifolia). We injected male Jiaobai plants at the five-leaf stage with U. esculenta suspension, and leaves at different timepoints after inoculation were collected for transcriptome sequencing. Transcriptome sequencing identified 1,226 DEGs mainly enriched in resistance-related processes. Weighted gene coexpression network analysis (WGCNA) showed that ‘plant–pathogen interaction’ and ‘MAPK signalling pathway-plant’ were the two most important upregulated pathways, and 16 candidate genes related to response to U. esculenta infection were screened. The results provide a basis for determining the molecular mechanisms of the response of male Z. latifolia to U. esculenta infection.

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Microbial Diversity in the Edible Gall on White Bamboo Formed by the Interaction between Ustilago esculenta and Zizania latifolia

Cited by 11 publications

References 21 publications

Antifungal activities of vineyard-habitat wild yeast for grape gray-mold disease and its initial promotion of alcohol fermentation in spontaneous wine brewing

Antifungal activities of vineyard-habitat wild yeast for grape gray-mold disease and its initial promotion of alcohol fermentation in spontaneous wine brewing

Antifungal activities of vineyard-habitat wild yeast for grape gray-mold disease and its initial promotion of alcohol fermentation in spontaneous winemaking

MAP kinase and plant–pathogen interactions govern male Zizania latifolia responses to Ustilago esculenta during the early stages of infection

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