Microbial ecology and predicted metabolic pathways in various oral environments from patients with acute endodontic infections

Moraes, Ludmila Coutinho; Lang, Pauline Mastella; Arcanjo, Rafaela Alves; Rampelotto, Pabulo Henrique; Parolo, Clarissa Cavalcanti Fatturi; Ferreira, Maria Beatriz Cardoso; Montagner, Francisco

doi:10.1111/iej.13389

Cited by 11 publications

(9 citation statements)

References 57 publications

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“…Antibiotics not only affect the composition and functions of the oral microbiome, but also induce specific metabolic changes during antibiotic interventions (Moraes et al, 2020). Like previous studies (Monroy-Pérez et al, 2020), a prospective cohort study found that Shannon's diversity index was reduced by treatment with amoxicillin relative to that in the untreated group, and decreased continuously for 6 months (Menon et al, 2019).…”

Section: Antibiotic Utilizationmentioning

confidence: 71%

The Oral Microbiota: Community Composition, Influencing Factors, Pathogenesis, and Interventions

et al. 2022

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The human oral cavity provides a habitat for oral microbial communities. The complexity of its anatomical structure, its connectivity to the outside, and its moist environment contribute to the complexity and ecological site specificity of the microbiome colonized therein. Complex endogenous and exogenous factors affect the occurrence and development of the oral microbiota, and maintain it in a dynamic balance. The dysbiotic state, in which the microbial composition is altered and the microecological balance between host and microorganisms is disturbed, can lead to oral and even systemic diseases. In this review, we discuss the current research on the composition of the oral microbiota, the factors influencing it, and its relationships with common oral diseases. We focus on the specificity of the microbiota at different niches in the oral cavity, the communities of the oral microbiome, the mycobiome, and the virome within oral biofilms, and interventions targeting oral pathogens associated with disease. With these data, we aim to extend our understanding of oral microorganisms and provide new ideas for the clinical management of infectious oral diseases.

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Section: Antibiotic Utilizationmentioning

confidence: 71%

The Oral Microbiota: Community Composition, Influencing Factors, Pathogenesis, and Interventions

et al. 2022

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“…This change results in a higher number of S. Mutans in the oral cavity. 48 In our study, the effect of insulin resistance on oral flora can be thus explained.…”

Section: Discussionmentioning

confidence: 76%

“…51 An increase in the S. Mutans count is detected in the oral microflora when saliva flow rate decreases. 48 In our study, the effect of PCOS on the oral flora can be explained based on these reasons.…”

Section: Discussionmentioning

confidence: 77%

Assessment of DMFT Indexes, Salivary Flow Rate, Ph, and Detections of S.MUTANS Salivary Levels by a Quantitative Real-Time PCR in Polycystic Ovary Syndrome

HİLALOĞLU¹,

Sürmelioğlu

2022

Cumhuriyet Dental Journal

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Objectives: PCOS is an endocrine disorder that is common in women. However, PCOS effects on oral and dental health have not been stated clearly. The aim of this study is to examine the effects of Polycystic Ovary Syndrome (PCOS), which is common in women of reproductive age, on saliva and dental tissues in these women. Materials and Methods: One-hundred individuals who were / were not diagnosed with PCOS and insulin resistance were included in this study (n=100). Subsequently, individuals, with PCOS and insulin resistance (PCOSID +), with PCOS and non-insulin resistance (PCOSID-), without PCOS and insulin resistance (ControlID +) and without PCOS and non-insulin resistance (ControlID-) were divided into 4 groups (n=25). DMFT (Decayed, Missing, Filled Teeth) index was used for dental health evaluation, while pH meter was used for saliva pH measurement. Also, Streptococcus Mutans (S. Mutans) numbers were analyzed by the real-time Polymerase Chain Reaction (PCR) method. In statistical analysis p

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“…Prospective cohort studies revealed that Shannon biodiversity index was decreased during amoxicillin treatment and was subject to further reduction in the following 6 months’ time period ( Menon et al, 2019 ; Monroy-Pérez et al, 2020 ; Nel Van Zyl et al, 2022 ). The density of Neisseria, Streptococcus and Veillonella strains in the oral cavity was also reported to decrease during treatment with amoxicillin ( Larsson Wexell et al, 2016 ; Moraes et al, 2020 ). Save commonly used amoxicillin, other groups of antibiotics with various mechanisms of action can influence the oral microbiota and promote the selection of multi-drug resistant strains and their horizontal transmission ( Zaura et al, 2015 ; Moraes et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…The density of Neisseria, Streptococcus and Veillonella strains in the oral cavity was also reported to decrease during treatment with amoxicillin ( Larsson Wexell et al, 2016 ; Moraes et al, 2020 ). Save commonly used amoxicillin, other groups of antibiotics with various mechanisms of action can influence the oral microbiota and promote the selection of multi-drug resistant strains and their horizontal transmission ( Zaura et al, 2015 ; Moraes et al, 2020 ). Other factors that can lead to oral dysbiosis include: local and systemic diseases, improper oral hygiene, unbalanced diet, smoking tobacco and immunosuppression ( Li et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Cultivable oral bacteriota dysbiosis in mechanically ventilated COVID-19 patients

Gregorczyk-Maga

Fiema²,

Michal³

et al. 2022

Front. Microbiol.

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Potential interactions between the SARS-CoV-2 virus and the human oral microbiota are currently investigated widely. Patients with COVID-19 requiring mechanical ventilation in an intensive care unit (ICU) setting are at high risk of developing severe complications, including ventilator-associated pneumonia, thus making oral health management important. The aim of this study was to evaluate the oral health status and assess the dysbiosis of cultivable oral bacteriota in COVID-19 patients hospitalized in an ICU with acute respiratory distress within 36 h following intubation. In this prospective cohort study, we recruited 56 adult COVID-19 patients that qualified for mechanical ventilation in the Temporary ICU for COVID-19 Patients of the University Hospital in Krakow. On admission to the ICU, oral health of patients was assessed using the modified Beck Oral Assessment Score (BOAS). Four oral habitats were sampled, namely the buccal mucosa, tongue, buccal dental surface and gingival pocket. Microorganisms were identified by MALDI/TOF mass spectrometry. The mean age of the study population was 66.5 ± 12.7 years, there were 24 (42.9%) females. All patients included in this study were intubated and ventilated in the ICU, with a corresponding high mortality rate (76.8%). On admission to ICU, 76.8% subjects scored 11–20 on the BOAS scale (median 12 [IQR 10–14]), indicating moderate or severe dysfunction of oral health. Potentially pathogenic bacteria were identified in the oral microbiota samples, including Acinetobacter baumannii, Enterococcus faecalis, Escherichia coli and Klebsiella pneumoniae in 23.2%, 39.3%, 17.9%, and 19.6% of patients, respectively. Lactobacillus spp. were present in 57.1% subjects. The mean CFU counts of all bacteria strains in dental brushes were 9.3E+5 (1.4E+6) and in gingival pockets 7.6E+5 (1.4E+6). The highest CFU counts were observed for Enterococcus spp. and, Lactobacillus spp., although these did not differ significantly from CFU counts of Streptococcus spp. and Staphylococcus spp. In this report we comprehensively characterized the oral health condition and cultivable oral bacteriota in COVID-19 patients hospitalized in an ICU with acute respiratory distress within 36 h following intubation. The oral bacteriota showed significant qualitative and quantitative dysbiosis. Hospitalization in an ICU and mechanical ventilation are important factors leading to oral dysbiosis in SARS-CoV-2 patients.

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Microbial ecology and predicted metabolic pathways in various oral environments from patients with acute endodontic infections

Cited by 11 publications

References 57 publications

The Oral Microbiota: Community Composition, Influencing Factors, Pathogenesis, and Interventions

The Oral Microbiota: Community Composition, Influencing Factors, Pathogenesis, and Interventions

Assessment of DMFT Indexes, Salivary Flow Rate, Ph, and Detections of S.MUTANS Salivary Levels by a Quantitative Real-Time PCR in Polycystic Ovary Syndrome

Cultivable oral bacteriota dysbiosis in mechanically ventilated COVID-19 patients

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