Microbial, Enzymatic, and Immune Biosensors for Ecological Monitoring and Control of Biotechnological Processes

Abstract

High specificity and sensitivity are characteristic of immunosensors; however, the assay time presents a certain limitation in immunoassay detection. Considering possible methods of shortening the assay time, we proposed and experimentally realized a new type of immunosensor detection. This method was tested by the Abstract -Results of the research performed at the Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, on designing immunobiosensors for detection of toxic compounds and microbial cells, enzyme-based biosensors for detection of hydrocarbons and alcohols, and microbial biosensors for aromatic compounds, surfactants, and biological oxygen consumption are reviewed. Parameters of the mediator electrodes involving microbial cells and data on the properties of microbial biofuel cells-devices based on the biosensor principle and representing alternative sources of electric energy-are presented.

Fundamentals, Applications, and Future Directions of Bioelectrocatalysis

“…587 To date, substrate specificity characteristics of several bacterial strains have been investigated with a number of substrates, such as organic acids, carbohydrates, and alcohols, and compiled in a database. 588 These data enable the selection of appropriate microorganisms in selecting microbial biosensor components. For instance, a few research studies have used substrate specificity properties from this database for Gluconobacter species in the development of potentiometric and amperometric microbial biosensors for the detection of xylose.…”

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

“…Challenges associated with matrix-induced loss of sensitivity and specificity have led to the innovation of the technology leading to self-contained immune-based biosensors and nano-based assays. These biosensors convert the binding activities of the antibody into an electrical signal that is more precise for the assessment of the pathogen in a mixed culture (O’Connell et al., 2000; Willner, 2002; Reshetilov, 2005; Wei et al., 2007; Huang et al., 2010; Masdor et al., 2016; Alahi and Mukhopadhyay, 2017; Asal et al., 2018). In a more recent study, Masdor et al.…”

Microbial Biosensors for Environmental Monitoring and Food Analysis

“…The substrate specificities were assessed with respect to a wide range of carbohydrates, alcohols, and organic acids and it was found these data may be effectively applied for the selection of appropriate strains as the major components of biosensors. (10) Studies have designed two types of biosensors for the detection of xylose using data on the substrate specificities of Gluconobacter strains gathered from the compiled database. The first biosensor utilized potentiometric signal recording, with a lower xylose detection limit of 0.5 mM and a linear calibration dependence in the range of 5.0 to 30.0 mM.…”

“…For this purpose, a database has been compiled that contains the substrate specificity characteristics of various microorganisms belonging to different taxa. (10) For example, the properties of 25 strains were examined to find the most efficient microorganisms to serve as receptor elements for amperometric biosensors. The substrate specificities were assessed with respect to a wide range of carbohydrates, alcohols, and organic acids and it was found these data may be effectively applied for the selection of appropriate strains as the major components of biosensors.…”